The same restriction endonuclease must be used to excise the foreign DNA and bacterial DNA. Select one: True False Why? Select one: a. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are different. b. It must use same restriction endonuclease so that the restriction sites are identical in both foreign and bacterial DNA. c. It must use same restriction endonuclease so that the restriction sites are different in both foreign and bacterial DNA. d. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are exactly the same.

The same restriction endonuclease must be used to excise the foreign DNA and bacterial DNA. Select one: True False Why? Select one: a. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are different. b. It must use same restriction endonuclease so that the restriction sites are identical in both foreign and bacterial DNA. c. It must use same restriction endonuclease so that the restriction sites are different in both foreign and bacterial DNA. d. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are exactly the same.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 8QP

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You are trying to clone a gene, You have successfully isolated it from the genomic DNA of an organism using the Hindill restriction enzyme. You then take a plasmid with a single EcoRI restriction site and cleave it with EcoRI. You combine these two fragments and treat them with DNA ligase. Answer the two questions below. a. Does the cloning reaction succeed as described? If so, what is the product obtained? b. Explain your answer above,
The gene you are asked to clone is 30,000 bps in length. When you are choosing a suitable Restriction Endonuclease, what criteria about the enzyme can you deduce from the gene length?
When joining two or more DNA fragments, a researcher can adjust the sequence at the junction in a variety of subtle ways, as seen in the following exercises.(a) Draw the structure of each end of a linear DNA fragment produced by an EcoRI restriction digest (include those sequences remaining from the EcoRI recognition sequence).(b) Draw the structure resulting from the reaction of this end sequence with DNA polymerase I and the four deoxynucleoside triphosphates.(c) Draw the sequence produced at the junction that arises if two ends with the structure derived in (b) are ligated (d) Draw the structure produced if the structure derived in (a) is treated with a nuclease that degrades only single-stranded DNA.(e) Draw the sequence of the junction produced if an end with structure (b) is ligated to an end with structure (d).(f) Draw the structure of the end of a linear DNA fragment that was produced by a PvuII restriction digest (include those sequences remaining from the PvuII recognition…
Restriction enzymes and DNA ligase play essential roles in DNA cloning. How is it that a bacterium that produces a restriction enzyme does not cut its own DNA? Describe some general features of restriction sites.
Restriction enzymes and DNA ligase play essential roles in DNA cloning. How is it that a bacterium that produces a restriction enzyme does not cut its own DNA?
A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the plasmid with Xhol and Xbal, which lane of the agarose gel represents the DNA fragments you would expect from the digestion? B. If you now decide to cut the plasmid with EcoRI, how many fragments will be produced and what will their sizes be? C. When running DNA samples on agarose gel, an electric field is applied. Towards which electrode will the DNA migrate and why?
If you were doing a cloning experiment where a plasmid was treated with PstI but the insert DNA did not have a PstI restriction site, what restriction enzyme(s) would be suitable to treat the DNA to be inserted?
State the characteristics that would be required for using a restriction endonuclease with human DNA, and explain why you needed to use the empty DNA fragment.
Which of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of the DNA to be amplified must be known. b) the sequence of restriction endonuclease recognition sites in the DNA to be amplified and in the plasmid, where the amplified DNA fragment will be cloned must be known. c) The complete sequence of the DNA to be amplified must be known. d) The sequence of restriction endonuclease recognition sites in the DNA to be amplified must be known
Which restriction enzyme used in your simulated electrophoresis experiment produced DNA with ‘sticky ends’? Which produced blunt ends? Of these two restriction enzymes, which would you choose to use as donor DNA to graft (or splice) onto a recipient strand of DNA, and why?
How would the results of this activity have been different if the DNA sequence you digested were circular instead of linear? When restriction digests are performed and analyzed in the laboratory, one lane of the agarose gel is loaded with DNA ladders, which are DNA fragments of known sizes. What is the point of including these size standards?
A small DNA molecule was cleaved with several different restriction nucleases, and the size of each fragment was determined by gel electrophoresis.The following data were obtained. (a) Is the original molecule linear or circular?(b) Draw a map of restriction sites (showing distances between sites) that isconsistent with the data given.(c) How many additional maps are compatible with the data?(d) What would have to be done to locate the cleavage sites unambiguouslywith respect to each other?