Using what you now know about restriction enzymes, why do you suppose that wells in the gel contained more than one band?

Q: How would restriction enzymes play a role in having an organismproduce a protein that it normally…

A: Restriction enzymes are specialized proteins produced by the bacteria that cut the DNA at a specific…

Q: What bacteria were used for the isolation of the three enzymes (HindIII, NdeI and PvuI)? What type…

A: HindIII  is isolated from Haemophilus influenzae . NdeI is an  isolated from Neisseria…

Q: Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only…

A: PCR polymerase chain reaction is a biotechnology technique used for amplification of DNA.

Q: Why do prokaryotes produce restriction enzymes? A. Restriction enzymes are produced to enable…

A: Restriction enzymes cut the DNA at specific recognition sequences.

Q: Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the…

A: Vast uses of endonuclease activity of restriction enzyme found in the biotechnology for gene…

Q: What do restriction enzymes do

A: What do restriction enzymes do ?

Q: What is the job of the restriction enzyme?

A: In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli…

Q: DNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes…

A:

Q: How would a researcher determine if a new species of bacteria contains the gene for producing DNA…

A: The prolonged exposure of DNA to UV radiation will result in mutation, where the thymine residues…

Q: Explain the basis for identification using DNA fingerprinting. – relate this to Microbiology not to…

A: DNA fingerprinting is a chemical test that shows the genetic makeup of a person or other living…

Q: What is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic…

A: Restriction enzymes are involved in accomplishing various biotechnological processes. Mostly found…

Q: Mention two classes of restriction enzymes. Suggest their respective roles.

A: The enzyme mainly involved in recombinant DNA technology are restriction enzymes, ligases, lyases,…

Q: Why do bacteria make restriction endonucleases?

A: Deoxyribonucleic acid (DNA) is a double-stranded molecule, which consists of two strands of…

Q: Which two restriction enzymes where used to completely digest the DNA fragment above based on the…

A: Restriction endonucleases are the enzymes which cleave the DNA at specific sites from within and…

Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…

A: Gel electrophoresis is used to separate molecules of different sizes. It uses charged molecules that…

Q: a restriction enzyme is one that?

A: A restriction enzyme is a most important tool in recombinant technology. The alteration of the…

Q: Why do eukaryotic cells do not contain restriction enzymes?

A: Restriction enzymes are used extensively in molecular biology to cut DNA. One of the unique features…

Q: what is the function of restriction enzymes in digesting products in PCR

A: Restriction enzymes are also known as molecular scissors because they involves in digestion of…

Q: What are restriction enzymes?

A: Restriction enzymes are found in bacteria, their function is to recognise and cut a specific site on…

Q: What is the function of the Ti plasmid in the creation of transgenic organism? Explain your answer.

A: A plasmid can be defined as the extrachromosomal DNA of the bacterial which is small and separated…

Q: How could you determine if the white stringy material was DNA?   A. By using spectrophotometer…

A: Introduction The Hereditary Substance In Humans And Almost All Other Animals Is DNA, Or…

Q: molecular scissors", justify that

A: Restriction enzymes are found in a wide variety of prokaryotes. Restriction enzymes received their…

Q: Which enzyme was used to produce the molecule in the figure above? O ligase O DNA polymerase O RNA…

A: DNA cloning, is a process in which a piece of gene is inserted into the circular piece of DNA…

Q: Which of the DNA sequences shown below can be cut by using restriction enzyme' Explain your answer…

A:

Q: What uses do Type 1 restriction enzymes have if they seem to be so inaccurate

A: Restriction enzymes refer to the restriction endonuclease enzymes that are used to cut DNA…

Q: Explain the Restriction Enzymes ?

A: An enzyme derived from microbes that breaks DNA molecules at specified sequences is known as a…

Q: restriction enzyme

A:

Q: If the following is a restriction enzyme: Sma I, A. What is the first letter represent? B. What do…

A: Sma I is a restriction enzyme. It cuts the DNA. The recognition sequence it recognizes is CCCGGG.

Q: Who did discover restriction enzymes and how did they discover it? And what experiments did they use…

A: It is the one of the tools of recombinant DNA technology which is known as molecular scissors. Today…

Q: Which are the Ten Commonly Used Restriction Enzymes?

A: A restriction enzyme could be a macromolecule that acknowledges a particular, short nucleotide…

Q: What is a reason that a cut digest insert would not match up in length with a PCR insert in gel…

A: Electrophoresis describes the separation of charged particles and their migration in the influence…

Q: Who isolated Restriction enzymes for the first time?

A: Restriction enzymes, also known as restriction endonucleases, recognize specific sites and cut DNA…

Q: Based on the image below, answer the following questions:   Restriction enzymes PstI and ScaI were…

A: Restriction enzymes  These enzymes have the capability to identify and cleave specific restriction…

Q: Why restriction enzymes are used ?

A: Introduction: Restriction enzymes are endonucleases that cleaves the DNA at specific sites. They are…

Q: Where do restriction enzymes come from?

A: Restriction enzymes are proteins that cuts DNA into fragments at restriction sites within a…

Q: How do you figure out which type of restriction enzymes to use in an experiment and what would…

A: Restriction enzymes are those that cleaves the DNA into fragments at or near specific sites within…

Q: a) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c)…

A: Enzymes are mainly used for the DNA manipulation or used in Recombinant DNA technology. there are…

Q: How do Restriction Enzymes like EcoRI work?

A: Restriction endonucleases  are the enzymes which is used to cut particular region of DNA . They make…

Q: How many restriction fragments did the digest produce in Lane 3? What does that mean about the…

A: In DNA before gel electrophoresis DNA is cut by various types of restriction endonucleases which are…

Q: a)List and describe the purpose of each component of the restriction enzyme digest.

A: Since you have asked multiple question, we will solve the first question for you. If youwant any…

Q: are restriction endonuclease enzymes named? Write examples.

A: Restriction enzymes break DNA at or near certain recognition sequences known as restriction sites,…

Q: How do the following factors affect the activity of restriction enzymes? pH conditions Mg2+…

A: Restriction enzymes are those enzymes which cut the DNA at specific places so that they can be used…

Q: Why don’t bacteria cut up their own DNA when they produce restriction enzymes?

A: Restriction enzymes are the proteins which can cut double stranded DNA segments only at some…

Q: How would you ascertain if your restriction experiment was successful?

A: Restriction digest of plasmid DNA is done using the naturally occurring restriction enzymes that…

Q: You isolate bacterial DNA from an unknown species, and use a PCR primer that amplifies DNA only from…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Diagram and describe the methods of PCR, DNA sequencing, Restriction Enzymology, & Hybridization…

A: A gene is the essential physical and functional unit of heredity. Genes are comprised of DNA. A…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.