Please answer both parts, a. The enzyme that catalyzes the joining of fragments to form recombinant DNA is: Question 21 options: DNA polymerase DNA ligase helicase restriction endonuclease b. In the above diagram, ‘1’ represents the Question 3 options: sticky ends restriction sites primer restriction fragments

Q: Your company has decided to develop a PCR test that will be used to determine Huntington's disease.…

A: The polymerase chain reaction (PCR), is one of the most useful molecular biology techniques used to…

Q: Second Base Analyze the following DNA sequences: A UUU UCU UAU UGU Phe Tyr UUC UCC UAC UGC Original:…

A: So, the given information is:- ORIGINAL- AGAGAGAGAGAGAGAGAG…

Q: digest suspect B's DNA using restriction enzyme 1. Restriction enzyme 1 cleaves the recognition site…

A: The restriction enzymes are endonucleases that digest DNA (deoxyribonucleic acid) molecules by…

Q: The red line indicates where a radioactively labeled probe hybridizes. Three samples of the genomic…

A: P                      B                         PB…

Q: Figure 9-22 shows the first steps in the process of making a DNA microarray, or DNA chip, using…

A: A DNA microarray is also known as the DNA chip, is the high throughput method that is used to trace…

Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…

A: An enzyme that recognizes a specific sequence on the DNA strand and cuts the DNA into fragments is…

Q: Why is it necessary to chelate the metal ions from the solution during the boiling/lysis step at 100…

A: The polymerase chain reaction (PCR) is a fundamental biochemical method for exponentially…

Q: Please answer both Part a. The plasmid you used as a template in your positive control for your PCR…

A: The Annealing Temperature is quite less in this case and hence there will not be proper or full…

Q: Restriction mapping of the delta chromosome I need help with question two please

A: the mean size is close to 4000 bp. DNA fragments of this length are useful in the lab, since…

Q: Can you please check my answer and make sure it is correct.   Question: Describe the two roles…

A: Primers provide specificity for amplification of the target sequence. They are typically 18-30…

Q: Question 1. Restriction endonucleases can be isolated from a number of bacteria. In bacteria…

A: Restriction endonucleases are DNA cutting enzymes. They cleave the DNA at or near specific sequence…

Q: (a) From your analysis of the pattern of bands on the gel, draw the correct restriction map and…

A: Because this is linear DNA, a single enzyme cut will lead to two fragments, and because all of the…

Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…

A: The corner stone of genetic engineering or recombinant DNA technology is a special class of enzymes…

Q: Amplified target regions of four different samples were separated using gel electrophoresis.  DNA…

A: Gel electrophoresis is a lab technique for separating DNA, RNA, and protein mixture based on their…

Q: The following image will be used to answer questions 1 through 9. Below is a restriction map of the…

A: Restriction enzyme and the type of enzyme which cut specific nucleotide sequence on double stranded…

Q: Tell me how did you prepared the gel if the tray is 150 ml? how many milliliters of the TBE and how…

A: The answer to the first question is, 3 grams of agarose in 150 ml of TBE.   *to get the second…

Q: The following image will be used to answer questions 1 through 9. Below is a restriction map of the…

A: Introduction :-  Restriction endonuclease enzymes are the enzymes which cuts the double stranded DNA…

Q: A piece of DNA 5.0 kb long is cloned and then cut out of the vector for analysis. This linear piece…

A: Restriction enzyme is any enzyme that cleaves DNA at a particular sequence. Example - BamHI, EcoRI,…

Q: QUESTION 7 Primers are needed to start a PCR reaction True O False QUESTION 8 Restriction enzymes…

A: Introduction:- Restriction enzyme is a  protein that recognise a specific, short nucleotide sequence…

Q: You performed RADseq analysis of two genomes using the restriction enzyme EcoRI. From each genome,…

A: * Restriction site associated DNA (RAD) markers are genetic marker that are used for association…

Q: Identify the basic steps (in right order) in AFLP marker analysis. Explain briefly how this marker…

A: Basic steps in AFLP marker analysis.

Q: Explanation of why mutations vary in likelihood of causing disease depending on location relative to…

A: Phenylketonuria is caused due to a genetic mutation in the PAH gene( phenylalanine hydroxylase…

Q: The figure below shows the recognition sequences and cleavage positions of three restriction…

A: Restriction enzymes are those that have a tendnecy to cut the strands of DNA straight. This leads to…

Q: Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences,…

A: Answer ::   Introduction A plasmid is a genetic structure in a cell that can replicate independently…

Q: tarting material, how many restriction sites were there considering you observed three bands after…

A: Restriction enzyme or restriction endonucleases act on certain specific sites present in the double…

Q: Why RT-PCR is important in the sample preparation to perform expression microarray experiment?

A: RT - PCR : Reverse transcription- polymerase chain reaction is one of the types of PCR.  DNA…

Q: Another restriction enzyme is called HaellI. It cuts DNA at the following base sequence: CCGG GGCC…

A: restriction enzymes are those enzymes which helps in cutting dna at specific locations .

Q: Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences,…

A: Introduction A plasmid is a genetic structure in a cell that can replicate independently of its…

Q: Can you please check my answer and see if it is correct.    Question: In addition to master mix,…

A: Polymerase chain reaction (PCR) is a versatile and rapid in vitro technique for amplifying the gene…

Q: 1. What are the materials used for the polymerase chain reaction?  2. Draw a schematic diagram of…

A: The polymerase chain reaction process is used for the amplification of desired target DNA…

Q: Electrophoresis data: Measure the distance (in millimeters) that each fragment traveled from the…

A: Introduction Gel electrophoresis is a laboratory technique for separating DNA, RNA, and protein…

Q: Which of the DNA sequences shown below can be cut by using restriction enzyme' Explain your answer…

A:

Q: ARE THEY TRUE OR FALSE? a) In a caesium chloride density gradient centrifugation method performed…

A: DNA is the genetic material that helps to control all the activity of the cell. The replication,…

Q: Can you please check my answer and make sure it is correct. Question: List the ingredients of master…

A: Polymerase chain reaction (PCR) is a technique to amplify the DNA. For successful PCR, the following…

Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…

A: The plasmid map can be created by checking the individual band patterns and band patterns with a mix…

Q: f restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up…

A: Nucleases are enzymes that degrade nucleic acids. They can be classified into RNase   : enzymes…

Q: Please answer asap and type your answer and do not copy from anywhere please NOTE*** double stranded…

A: Restriction enzymes These are also called molecular scissors. These enzymes are extracted from…

Q: Below are gel electrophoresis results for initial and nested PCR of the Shrimp Plant. Use the…

A: Electrophoresis is the process of separation of molecules on the basis of their charge/mass ratio…

Q: Describe the process for shotgun sequencing of a genome. Practice aligning the two sets of sequenced…

A: Since you have posted multiple questions, we solve the first question for you. To get the remaining…

Q: Can you help me with this question, please? What are the advantages of qPCR (RT-PCR) compared to…

A: PCR is a technique of DNA amplification in-vitro. The amplified DNA is usually visualized with gel…

Q: From your knowledge about DNA microarray, answer the following: A- How DNA microarray is created?…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three subparts…

Q: Table 1 shows a list of restriction endonucleases with their recognition sequence and the sites of…

A: Restriction enzymes are those that cleave the double stranded DNA at specific site. They have…

Q: For the analysis of plasmid DNA samples, ________ that recognize, bind to and cleave DNA at specific…

A: Restriction enzyme is a protein produced by the bacteria that cleaves DNA at specific sites…

Q: Consider the four extraction/purification methods .  a. Which extraction method would you use for a…

A: DNA isolated from various biological samples can be used for a vast array of downstream…

Q: Answer the following: • What is DNA fingerprinting? Name 2 applications of DNA fingerprinting from…

A: Molecular markers are used for the differentiation of closely related and distantly related…

Q: A linear DNA fragment was produced by digestion with the restriction enzyme, Xba1. This fragment…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: A typical polymerase cycle reaction (PCR) program (Figure 3) followed as: HOLD 1 HOLD 3 HOLD 2…

A: PCR or polymerase chain reaction is a method in which polymerase enzyme is used so as to synthesise…

Q: advantages of using larger restriction fragments when constructing a genomic library of human DNA

A: DNA library A DNA library is a collection of cloned DNA fragments containing the gene of interest.…

Q: A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel…

A: The breaking apart or fragmentation of DNA strands is known as DNA fragmentation. It might be done…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.