The students have some wool samples that they have dyed. They want to find out how much of the dye washes out of the wool at different temperatures. They decide to use colorimetry. Describe a suitable method for this experiment. Include the control variables in your answer.
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- QUESTION - What information can you get from a residuals plot that has a pattern rather than a random appearance? PLEASE EXPLAIN ! THIS IS UV-VIS SPECTRUMPrevious question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.his is the result of an experiment in a spectrophotometer. please explain the graph (red and green one)
- You may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?
- What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and dilution factors? What are 2 disadvantages?What are the processes of concentrate samples analysis in Uv-visible spectroscopy? Please shortly answer at your own words(3-4 lines).previous question? At higher magnifications, the millimeter markings may be too far apart to appear together in the field of view. If this happens, use the measurement from a lower magnification to make an estimate.For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope used. - The answer for this question was 3/4mm
- This question is based on Bovine Serum Albumin I would like help on answering letters a-c. This lab examines the relationship between the absorbance of light by a solution at 595 nm and the concentration of the Coomassie Blue dye-BSA protein complex in the solution. State whether the following descriptions of the lab experiment are valid or not, and explain why you say Yes or No: a. The experiment would be significantly more accurate if absorbance readings were recorded for a range of wavelengths, not just for 595 nm b. The experiment has limited accuracy because it does not account for the absorbance of light by the other components (components that are not the dye-protein complex, such as excess dye that is not bound to any protein) of the solution. c. The absorbance reading measures practically all the protein content in the solutionsWhich of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourceThe experiment file says "Fill a cuvette with water and blank the spectrophotometer." What are we doing when we do this? Why is this step important