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Question options:
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Is ineffective for staining as DNA is soluble in acetic acids.
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Orcein can be extracted from three different species of flower.
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Staining occurs via hydrophobic bonding and van der Waals attractions.
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The active dyes in this strain are small, extremely hydrophobic anionic dyes.
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Solved in 3 steps
- Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninWhich of the following statements is FALSE? Question options: stains increase the contrast of a specimen with its surroundings all of these statements are TRUE dyes generally consist of a chromophore and an ion the chromophore of an acidic dye is positively-charged simple staining procedures use a single dye basic dyes bind to negatively-charged molecules
- Briefly explain the difference between Wright Staining and H&E Staining (why use one over the other)Why is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?How does electrophoresis separate dye pigments? What charge is carried by the pigments in this separation? Support your answer.
- Select all of the following that represent examples of positive stains. A) nigrosin B) methylene blue C) crystal violet D) India ink E) safraninWhat are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?What do you think there are 2 staining reagents applied in differential staining?
- In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the application of decolorizing agent, it leaves the acid fast cells colorless. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.When run under nondenaturing conditions (without SDS or β-mercaptoethanol), nativepolyacrylamide gel electrophoresis (PAGE) allows proteins to maintain biological activity, so the GFP band should fluoresce when exposed to UV light. How do the staining patterns of gels run in this way differ from the staining patterns of gels run using SDS-PAGE?Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 1. Simple Staining
