C The results of Motility Indole Ornithine (MIO) test - after the addition of Kovac's reagent - are shown used were: (A) E. coli, (B) E. aerogenes (C) B. subtilis, (D) Gram-negative cocci (unknown), and (E) Based on the above results, which of the following are positive for the production of indole frou tryptophan? (Select all that apply.) O A. E. coli B. E. aerogenes C. B. subtilis D. Gram-negative cocci E
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Which of the following are positive for the production of indole from the amino acid tryptophan?
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- The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?Which stationary phase is better in separation of the components of moringa extract for column chromatography? Sodium Bicarbonate or Silica Gel? And Explain.Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?
- You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.You did a serial dilution experiment to determine the CFU/table of the Webbers' probiotics. Given the ingredient list shown, how many colonies would you expect to see on a plate if you plated 0.1 mL from the 10-6 dilution tube, and incubated aerobically at 37 ⁰C for several days?An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?
- A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture into a 9 ml LB medium. After this, from 10-4 and 10-5 dilution tubes 100µl were plated onto LB agar plates. Upon overnight incubation at 37°C, 200 colonies were counted in 10-4 and 22 colonies were present on 10-5 plates. How many colony-forming units were present per ml of the original culture? If the formula for CFU/ml =no. Of colonies/dilution factor*volume of culture plateIn a mixture of five proteins listed, draw an elution profile (Absorbance vs. mL eluted, arbitrary) for the purification of the listed proteins on a gel filtration chromatography resin: cytochrome c (pI = 5.4; Mr = 13,000), immunoglobulin G (pI = 7.3; Mr = 145,000), ribonuclease A (pI = 9.6; Mr = 13,700), RNA polymerase (pI = 6.3; Mr = 450,000), human serum albumin (pI = 5.4; Mr = 68,500). Label your elution peaks. Draw a sketch of an SDS-PAGE, reflecting the mobility of the above mixture as they elute from the column. Label you protein bands.Hypothetically make the Dichotomous key for Micrococcus luteus, Bacillus subtilis, Bacillus, Megaterium, Bacillus cereus, E.coli, Serratia Marcescens, and Enterobacter aerogenes based on colony morphology. results of catalase test, (lactose, sucrose and glucose fermentation tests) and other biochemical tests for its accurate identification.
- What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for? What tool is used to inoculate this medium?In the context of flow cytometry data: It is clear that Lactobacillus cells can be detected based on their FSC (forward scatter) and SSC (side scatter) signal since it is above the noise. Thus, they could be quantified using these parameters without staining. Give two reasons as to why anyone would bother SYBRGreen (SG) staining samples that contain heterotrophs?In your own words, kindly give the function and allowable limit (if applicable) of each of the following: 1. Garlic 2. Onion 3. Ground pork 4. Salt 5. Pepper