Upon mixing CFP-a-p34 with GFP-a-p34 and 3-p34, the fluorescence of CFP drops from 100,000 to 96,000. When CFP-a-p34 is mixed with GFP-B-p34 in the presence of 0.5M GdnHCl the fluorescence of CFP drops from 100,000 to 63,900. If the Förster radius for the donor/acceptor pair is 50Å, how far apart are the donor and acceptor in each case?

Upon mixing CFP-a-p34 with GFP-a-p34 and 3-p34, the fluorescence of CFP drops from 100,000 to 96,000. When CFP-a-p34 is mixed with GFP-B-p34 in the presence of 0.5M GdnHCl the fluorescence of CFP drops from 100,000 to 63,900. If the Förster radius for the donor/acceptor pair is 50Å, how far apart are the donor and acceptor in each case?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter12: Atomic X-ray Spectrometry

Section: Chapter Questions

Problem 12.12QAP

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