Upon the addition of diazonium salts, ketoacids like glutamate and pyruvate forms in the presence of hydroxide as a color developer in Reitman Frankel Method. At pH 6.8, Pyruvate kinase catalyzes the transfer of phosphate from phosphoenolpyruvate to ADP to become ATP a. both false b. both are true c.. only second is true
Q: The flux through a metabolic pathway with 10 enzymes increases by 10% which the concentration of enz...
A: Enzymes are biocatalysts which increase the rate of a biochemical reaction by lowering the activat...
Q: What advantage do alternative sigma factors have for bacterial gene expression?
A: Sigma factors are dissociable subunits of prokaryotic RNA polymerase that are required for its funct...
Q: 1. A 50-ycars-old man consumes daily about 90 g of fat of animal origin (fatty mcat, butter, cheese)...
A: Triacyl glycerols (TAGs) are formed when the three -OH groups of glycerol are esterified with fatty ...
Q: In moelcular genetics, initiation is often accomplished using proteins that prevent elongation. Nam...
A: Initiation of a process in genetics is an important event. At this stage, cell prepare itself for th...
Q: A polypeptide with a pH of 2.0 has a total net charge of 0. Gly-Pro-Glu-Asp-Leu-His-Ile-Gln-Asn-Phe ...
A: This peptide is composed of glycine, proline, glutamic acid, aspartic acid, leucine, histidine, isol...
Q: classify the ff lipids whether it is simple,complex or derived: 1) lecithin 2) tallow 3) retinol 4...
A: Lipids: Lipids are a heterogeneous group of chemical compounds that includes fats, oils, waxes, ste...
Q: What are the main advantages of the presence of organelles in eukaryotic cells?
A: Cells are the basic units of life. Cells are broadly classified as prokaryotic and eukaryotic cells....
Q: The reverse synthesis of ceramides synthesized biologically from sphingosine is possible. 1. What ty...
A: Sphingolipids are a type of lipid that plays an important role in the homeostasis of human cells, eu...
Q: How are blood glucose concentration affected by heavy alcohol consumption? Please explain with extre...
A: Alcohol plays major role on health. Alcohol can effect every organ of the body. In an average person...
Q: What is the p-value or critical value of this problem?
A: A p- value is used in hypothesis testing to support or reject the null hypothesis. The p-value compa...
Q: Consider the following free energy diagram for an uncatalyzed and enzyme-catalyzed reaction. Select ...
A: Those proteins or biological catalysts which help to speed up the chemical reaction are termed enzym...
Q: Please answer in detail ASAP
A: Carbohydrates are macronutrients and one of the three basic sources of energy for our bodies. They a...
Q: Draw the product of the reaction below. (Upload your answer here) H- но ? NaBH4 HO -H H- ČH2OH
A: Given structure :- D-GALACTOSE (monosaccharide) In presence of NaBH4 , REDUCTION reaction oc...
Q: Why do you think DNA is the genetic material used by eukaryotes instead of RNA?
A: Deoxyribonucleic acid (DNA): Nucleic acids are molecules that store hereditary information for perf...
Q: I have four amino acids: serine, histidine, alanine, and tyrosine. How many different primary struct...
A: Proteins are composed of amino acids linked together by peptide linkages. T he primary structure of ...
Q: Which of the following would not be considered a reason for the decline in fat oxidation with increa...
A: Beta oxidation is a catabolic process that occurs in both prokaryotes and eukaryotes in order to bre...
Q: What class of enzymes catalyze the phosphorylation of amino acid residues in proteins? a. kinase...
A: Kinases are enzymes that adds a phosphate group to molecules such as sugars or proteins. Phosphatase...
Q: 2. Circle & Name functional groups C-C-N. но CH2 CH H,C CH3 H HE HICI
A: Biomolecules are organic molecules made up of mainly carbon and hydrogen but there are other element...
Q: Why do we compute for TFM in soaps. How does one calculate for TFM? What is saponification? Show it...
A: 1. The TFM quantity interprets the quality of soap. It adds moisturizing properties to the product, ...
Q: When you are served with the dessert, you had an emergency call from home and you need to return ho...
A: From the production of food to consumption of food it goes through several steps which include m...
Q: What will be the color of saliva extraction with iodine in 3, 6 9, 12, 15, and 18 mins if: you put 5...
A: Carbohydrates are divided into 3 classes monosaccharide, disaccharide, and polysacchari...
Q: он но но- HO но. но. но- -OH Он A OH В
A: Isomerism : It is a phenomenon of accurance of different arrangements of the atoms within the sam em...
Q: Which one of the following correctly illustrates peptide bond formation in the peptidyltransferase c...
A: The ribosomal peptidyl transferase center residing in the domain V of 23S rRNA (of the 50S subunit o...
Q: Consider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of c...
A: Sedimentation Coefficient is the rate per unit centrifugal field experienced by the particle undergo...
Q: You sequence a new tRNA and discover that the anticodon sequence is 5'-IGC-3. What amino acid does i...
A: I corresponds to an unusual bas hypoxanthine, which is found in tRNA
Q: Define anabolism
A: Metabolism is a biological process in which the food that the living body eats gets converted into e...
Q: WHAT ARE THE ADVANTAGES AND DISADVANTAGES OF CARBOHYDRATES IN BIOCHEMISTRY? GIVE AT LEAST 3 FOR EACH...
A: Carbohydrates are the one of the biomolecules that provide energy to the human body. They are mainly...
Q: Enzymes are catalysts that increase the rate of reactions by pulling two substrates together. low...
A: Catalysts speed up the chemical reaction without being changed themselves. All living organisms prep...
Q: Which of the following is cytidine? cytosine + guanine ribose + cytosine + phosphate group ...
A: Cytidine is a nucleoside monomer formed when a cytosine gets attached to a ribose sugar via a glycos...
Q: WHAT ARE THE DIFFERENCES OF MONOSACCHARIDES, DISACCHARIDES AND POLYSACCHARIDES IN TERMS OF: STRUCTUR...
A: Carbohydrates are macromolecules composed of carbon, hydrogen, and oxygen. Carbohydrates are classif...
Q: proteins are deamidated in an aqueous solution to produce ammonium and aspartate, respectively, from...
A: Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different m...
Q: What are the important metabolites/cofactors and where do they come from/how do they enter the citri...
A: The citric acid cycle also known as tri-carboxylic acid cycle (TCA Cycle) or Kreb's Cycle occurs in ...
Q: What is the monomoric form of actin referred to as? a. F-actin b. G-actin c. M-actin ...
A: Actin exists in two forms. They are G-actin (or globular actin) and F-actin (or fibrous actin). Acti...
Q: What are the 4 levels of structural organization of proteins and what are the important interactions...
A: The four levels of structural organization of proteins are : Primary structure, Secondary structure,...
Q: In a sample dsDNA from an organism, the amount of thymine is analyzed to be 12 μmoles when the A+T/G...
A: In double stranded DNA the composition of bases follows the rule: A+T= G+C.
Q: A conjugate was incubated with a mixture of lysosomal enzymes and no drug release was observed in th...
A: Lysosomes contain a wide variety of hydrolytic enzymes including proteases, nucleases, glycosidases,...
Q: State the differences between "planar" and "column" stationary phases and provide examples of each.
A: Chromatography techniques are based on the stationary phases used in separation.
Q: Figure 1 show the shape of the peaks in gas chromatography that causes loss of column efficiency. De...
A:
Q: The fact the DNA bears the genetic information of an organism implies that A Base composition should...
A: All living organisms have cells, which are the most basic and essential unit of life. Cells are resp...
Q: Which nucleotide is shown in the picture above?
A: A nucleotide is made up of a phosphate group, a sugar and a nitrogenous base. ATP provides energy to...
Q: Based
A: Insulin binds to the receptor activates several cascade of events.
Q: 6. Kwashiorkor is the discase causcd by a deficiency of proteins in the diet that is adequate in cal...
A: Protein deficiency (in Kwashoirkor) and hence the lack of protein reduces the production of lipoprot...
Q: Q7. Kinetics were analysed for an enzyme in the absence and presence of inhibitor. In the absence of...
A: In competitive inhibition the inhibitor competes with substrate for binding to an active site. In no...
Q: which one is a part of protein coenzyme Select one: a. sodium b. sulphur c. copper d. calcium
A: Coenzyme: It is a small non-protein low-molecular-weight molecule that is bound tightly loosely to ...
Q: What are some examples of biochemical tests for proteins that involve acid hydrolysis or base hydrol...
A: Proteins: Proteins are complex high-molecular-weight molecules that consist of amino acids joined b...
Q: The nonessential amino acids cannot be produced from the other amino acids or from simple organic mo...
A: Amino acids are organic molecules and compose of carbon, hydrogen, oxygen, and nitrogen. It contains...
Q: A small peptide has two pKa values of 3.42 and 8.74. What is the isoelectric point for this peptide?...
A: Isoelectric point: The isoelectric point(pI) is the pH at which particular molecule carries no net...
Q: Below is the primary sequence of a viral protein. MSVVNTEIKFPTHLRSGDFAIIDGMVVEVTSVEYKPVEQAVYLKYRYHL...
A: Biomolecules are organic molecules made up of mainly carbon and hydrogen but there are other element...
Q: Under what conditions does Km represent the true binding affinity (i.e. Kd) of the substrate to the ...
A: Ans) option 1 is the correct answer. when kcat << k-1 km is the substrate concentrat...
Q: Soluble fiber attracts lots of water, swells, and becomes viscous. This characteristic allows it to ...
A: Dietary fibre is a component of plant-based foods that primarily flows through your digestive system...
Upon the addition of diazonium salts, ketoacids like glutamate and pyruvate forms in the presence of hydroxide as a color developer in Reitman Frankel Method. At pH 6.8, Pyruvate kinase catalyzes the transfer of phosphate from phosphoenolpyruvate to ADP to become ATP
a. both false
b. both are true
c.. only second is true
d. only first is true
2. constriction of the sphincter of oddi may cause falsely ______ in ____ activity
a. none of the choices
b. decresed, lipase
c. increased, ck-mb
d. increaased, amylase
e. decreased, LDH
3. The amyloclastic method of amylase activity determination refers to the measurement of the disappearance of the starch substrate when mixed with amylase in the sample. The addition of L-tartrate measurement of CP activity increases the sensitivuty of the substrate used to the prostatic ACP isoenzyme
a. both are true
b. both are false
c. only second is true
d. only first is true
Step by step
Solved in 2 steps
- DCCD (diocyclohexylcarbodiimide) inhibits oxidative phosphorylation when the substrate is mitochondrial NADH. DCCD is a drug that binds to ATP synthase and blocks proton transport through the ion channel. a) Explain what the consequences of DCCD on cellular energy production are. b) Suggest at least one other cellular effect of DCCD and explain this effect.Which of these is associated with production of ATP during glycolysis? (Select all that apply) a) Conversion of 1,3-BPG to 3PG b) Conversion of 1,3-BPG to 2,3-BPG c) Conversion of PEP to pyruvate d) Conversion of 2,3-BPG to 2PG e) Phosphorylation of glucosePatients with pyruvate dehydrogenase deficiency show high levels of lactic acid in the blood. However, in some cases, treatment with dichloroacetate (DCA) lowers lactic acid levels. (a) How does DCA act to stimulate pyruvate dehydrogenase activity? (b) What does this suggest about pyruvate dehydrogenase activity in patients who respond to DCA?
- The effect of ATP on the allosteric enzyme PFK-1 is shown below. For a given concentration of fructose 6-phosphate, the PFK-1 activity increases with increasing concentrations of ATP, but a point is reached beyond which increasing the concentration of ATP inhibits the enzyme. (a) Explain how ATP can be both a substrate and an inhibitor of PFK-1. How is the enzyme regulated by ATP? (b) In what ways is glycolysis regulated by ATP levels? (c) The inhibition of PFK-1 by ATP is diminished when the ADP concentration is high, as shown in the illustration. How can this observation be explained? *A graph is included for this question*Intramitochondrial ATP concentrations are about 5 mM, and phosphate concentration is about 10 mM. Consider that ADP is five times more abundant than AMP. a. Calculate the molar concentrations of ADP and AMP at an energy charge of 0.85. b. Calculate ∆G' for ATP hydrolysis under these conditions (∆G0' for ATP hydrolysis is -32.2 kJ/mol) The energy charge is defined as ( [ATP] + 1/2 [ADP] ) / ( [ATP] + [ADP] + [AMP] )About the process of industrial production of ethanol by the yeast Saccharomyces cerevisiae, mark the correct alternatives: (a) the cells must be cultured in anaerobic conditions to activate the metabolic pathway of ethanol production (b). the presence of oxygen is required to allow regeneration of the NAD+ cofactor (c). good oxygenation of the medium is important to favor the formation of greater amounts of ATP (d).the production of ethanol is always accompanied by the formation of glycerol (e). the sugar present in the culture medium is completely oxidized to CO2 and water
- Pyruvate is metabolized involving a multienzyme complex, pyruvatedehydrogenase complex (PDC).(a). What are the main products of PDC reactions?(b). Name two products that inhibit PDC reactions and give the reasons.Which of these molecules has a higher phosphoryl potential than ATP? a) glucose-6-phosphate b) glucose-1-phosphate c) phosphoenolpyruvate d) glyceraldehyde-3-phosphate The most commonly used biochemical reducing agent is: a) NADH b) NADPH c) FADH2 d) LiALH4 Hexokinase catalyzes: a) conversion of glucose to glucose-6-phosphate b) conversion of glucose-6-phosphate to fructose-6-phosphate c) conversion of fructose-6-phosphate to fructose-1,6-biphosphate d) cleavage of fructose-1,6-biphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate Which one of these molecules is not found in glycolysis? a) 1,3-biphosphoglycerate b) phosphoenolpyruvate c) pyruvate d) oxaloacetate The major regulatory enzyme of glycolysis is: a) hexokinase b) phosphofructokinase c) aldolase d) triose isomerase NADH is produced at which step in glycolysis? a) conversion of fructose-6-phosphate to fructose-1,6-biphosphate b) cleavage of fructose-1,6-biphosphate…Statins are competitive inhibitors that target HMG CoA reductase to decrease plasma cholesterol. a) What effect statin has on the enzyme's Km, b) How can we reverse a competitive inhibitor's effect? a) Increases Km, b) Increasing the [S] a) decreasing Km, b) Increasing the [S] a) Increases Km, b) decreasing the [S] a) No effect on Km, b) Inhibitor cannot be reversed
- Pyruvate dehydrogenase deficiency, a severe condition with a poor prognosis, results in neurological dysfunction and unremitting lactic acidosis. One treatment is placing the patients on a low carbohydrate/high fat diet. ( a) Explain why the deficiency would be characterized by neurological dysfunction. ( b) Why does lactic acidosis result? ( c) Explain the rationale for the low carbohydrate/high fat diet.Glucosamine is a common compound that inhibits hexokinase action (the transfer of a phosphate group to glucose during glycolysis). A) Describe the consequences of glucosamine exposure on cellular respiration. B) Is glucosamine exposure fatal? Why or why not?Which of the following stimulates the conversion of pyruvate to acetyl-CoA? a) Activation of pyruvate dehydrogenase kinase b) A decrease in the ratio of NAD+/NADH c) Bothaandb d) Neither a nor b