Use curve arrows to show the mechanism for the proposed conversion of 2a, 2b, 2c, and 2d to 4.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter18: Glycolysis
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Problem 25P: Using the ActiveModel for phosphofructokinase (Trypanosoma), describe the difference between the...
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Use curve arrows to show the mechanism for the
proposed conversion of 2a, 2b, 2c, and 2d to 4.
Based on the evidence presented, which is the most
likely pathway?
Explain the rationale for your choice.
Scheme 1
H
Но
HN
H+
02 + 2 H+ + 2 e-
+ H20
ÖH* H,O
1
2a
3
wwhm = a nucleophilic site on glutathione/cellular protein
Scheme 2
HN
+ H,O
H+
02 + 2 H+
+ NADPH
+H,0 + NADP
2b
3
mhm = a nucleophilic site on glutathione/cellular protein
Scheme 3
Bio.
BioH
BioH
Bio•
20
HN
X.
3
Bio•
H
RioH
N.
4
O:
Transcribed Image Text:Use curve arrows to show the mechanism for the proposed conversion of 2a, 2b, 2c, and 2d to 4. Based on the evidence presented, which is the most likely pathway? Explain the rationale for your choice. Scheme 1 H Но HN H+ 02 + 2 H+ + 2 e- + H20 ÖH* H,O 1 2a 3 wwhm = a nucleophilic site on glutathione/cellular protein Scheme 2 HN + H,O H+ 02 + 2 H+ + NADPH +H,0 + NADP 2b 3 mhm = a nucleophilic site on glutathione/cellular protein Scheme 3 Bio. BioH BioH Bio• 20 HN X. 3 Bio• H RioH N. 4 O:
Scheme 2
HN
+ H20
H+
02 + 2 H+
+ NADPH
HO
+H20 + NADP
2b
3
mhm = a nucleophilic site on glutathione/cellular protein
Scheme 3
Bio:
BioH
BioH
Bio•
20
HN
3
Bio•
BioH
4
OH
BioH
1
Bio•
2d
Bio = a biological molecule with a radical moiety such as heme, an Fe" containing coenzyme.
wwm =a nucleophilic site on glutathione/cellular protein
O:
Transcribed Image Text:Scheme 2 HN + H20 H+ 02 + 2 H+ + NADPH HO +H20 + NADP 2b 3 mhm = a nucleophilic site on glutathione/cellular protein Scheme 3 Bio: BioH BioH Bio• 20 HN 3 Bio• BioH 4 OH BioH 1 Bio• 2d Bio = a biological molecule with a radical moiety such as heme, an Fe" containing coenzyme. wwm =a nucleophilic site on glutathione/cellular protein O:
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