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L(24 points) Explain How is the Michaelis constant defined. and what does a low or high value for Km tell you? What is the difference between the velocity and initial velocity of an enzyme reaction? What determines the efficiency of an enzyme reaction, and what terms are used to describe it? 2. (50 points) About how to obtain kinetic data experimentally Lisa decides to obtain values for the Km and Vmax of an enzyme she has just isolated from liver cells (it is now pure), using a Michaelis Menten plot. Describe in detail what kinds of measurements she would have to make, and what she would need to plot on graphs in order to estimnte the values for Km and Vmax. (Show the kinds of graphs she would have to plot, and how these will allow her to estimate Km and Vmax.) Describe how she would be able to obtain Vmax experimentally and from the Michaelis Menten plot - what conditions are needed and what would be measured). Also, describe what she would have to do to obtain the turnover number of the enzyme. Consider what has to be known and controlled in carrying out the reactions needed. 3.(26 points) Interpreting enzyme characteristies from plots of Vo versus [S] Describe what you can learn about an enzyme from the plot given below. Estimate values for Km and Vmax, showing how you did the estimation. 10 no phonphoenolpyruvate Note that the substrate for the enzyme being examined is fructose-6- phosphate, and the molecule being added to the reaction is 0.8F with phosphoenolpyruvate 0.6 mas 0.4 phosphoenol pyruvate. 02 100 200 300 400 500 600 700 (Fructose-6-phosphate) (uM)

L(24 points) Explain How is the Michaelis constant defined. and what does a low or high value for Km tell you? What is the difference between the velocity and initial velocity of an enzyme reaction? What determines the efficiency of an enzyme reaction, and what terms are used to describe it? 2. (50 points) About how to obtain kinetic data experimentally Lisa decides to obtain values for the Km and Vmax of an enzyme she has just isolated from liver cells (it is now pure), using a Michaelis Menten plot. Describe in detail what kinds of measurements she would have to make, and what she would need to plot on graphs in order to estimnte the values for Km and Vmax. (Show the kinds of graphs she would have to plot, and how these will allow her to estimate Km and Vmax.) Describe how she would be able to obtain Vmax experimentally and from the Michaelis Menten plot - what conditions are needed and what would be measured). Also, describe what she would have to do to obtain the turnover number of the enzyme. Consider what has to be known and controlled in carrying out the reactions needed. 3.(26 points) Interpreting enzyme characteristies from plots of Vo versus [S] Describe what you can learn about an enzyme from the plot given below. Estimate values for Km and Vmax, showing how you did the estimation. 10 no phonphoenolpyruvate Note that the substrate for the enzyme being examined is fructose-6- phosphate, and the molecule being added to the reaction is 0.8F with phosphoenolpyruvate 0.6 mas 0.4 phosphoenol pyruvate. 02 100 200 300 400 500 600 700 (Fructose-6-phosphate) (uM)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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L(24 points) Explain How is the Michaelis constant defined. and what does a low or high value for Km tell you? What is the difference between the velocity and initial velocity of an enzyme reaction? What determines the efficiency of an enzyme reaction, and what terms are used to describe it? 2. (50 points) About how to obtain kinetic data experimentally Lisa decides to obtain values for the Km and Vmax of an enzyme she has just isolated from liver cells (it is now pure), using a Michaelis Menten plot. Describe in detail what kinds of measurements she would have to make, and what she would need to plot on graphs in order to estimnte the values for Km and Vmax. (Show the kinds of graphs she would have to plot, and how these will allow her to estimate Km and Vmax.) Describe how she would be able to obtain Vmax experimentally and from the Michaelis Menten plot - what conditions are needed and what would be measured). Also, describe what she would have to do to obtain the turnover number of the enzyme. Consider what has to be known and controlled in carrying out the reactions needed. 3.(26 points) Interpreting enzyme characteristies from plots of Vo versus [S] Describe what you can learn about an enzyme from the plot given below. Estimate values for Km and Vmax, showing how you did the estimation. 10 no phonphoenolpyruvate Note that the substrate for the enzyme being examined is fructose-6- phosphate, and the molecule being added to the reaction is 0.8F with phosphoenolpyruvate 0.6 mas 0.4 phosphoenol pyruvate. 02 100 200 300 400 500 600 700 (Fructose-6-phosphate) (uM)
Transcribed Image Text:L(24 points) Explain How is the Michaelis constant defined. and what does a low or high value for Km tell you? What is the difference between the velocity and initial velocity of an enzyme reaction? What determines the efficiency of an enzyme reaction, and what terms are used to describe it? 2. (50 points) About how to obtain kinetic data experimentally Lisa decides to obtain values for the Km and Vmax of an enzyme she has just isolated from liver cells (it is now pure), using a Michaelis Menten plot. Describe in detail what kinds of measurements she would have to make, and what she would need to plot on graphs in order to estimnte the values for Km and Vmax. (Show the kinds of graphs she would have to plot, and how these will allow her to estimate Km and Vmax.) Describe how she would be able to obtain Vmax experimentally and from the Michaelis Menten plot - what conditions are needed and what would be measured). Also, describe what she would have to do to obtain the turnover number of the enzyme. Consider what has to be known and controlled in carrying out the reactions needed. 3.(26 points) Interpreting enzyme characteristies from plots of Vo versus [S] Describe what you can learn about an enzyme from the plot given below. Estimate values for Km and Vmax, showing how you did the estimation. 10 no phonphoenolpyruvate Note that the substrate for the enzyme being examined is fructose-6- phosphate, and the molecule being added to the reaction is 0.8F with phosphoenolpyruvate 0.6 mas 0.4 phosphoenol pyruvate. 02 100 200 300 400 500 600 700 (Fructose-6-phosphate) (uM)
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