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Construct a Michaelis-Menten plot, and a Lineweaver-Burk plot, for all six of these experiments on the same graph (for each plot). Calculate the Vmax, the Km, and the slope (Km/Vmax) for the control (with [I] = 0 mM) and for each non-zero concentration of inhibitor C ([I] = 0.1, 0.2, 0.3, 0.4, and 0.5 mM). Which type of reversible enzyme inhibition is illustrated by inhibitor C? Since many of you do not have scanners or cameras to send me an e-mail image of your graphs,

Construct a Michaelis-Menten plot, and a Lineweaver-Burk plot, for all six of these experiments on the same graph (for each plot). Calculate the Vmax, the Km, and the slope (Km/Vmax) for the control (with [I] = 0 mM) and for each non-zero concentration of inhibitor C ([I] = 0.1, 0.2, 0.3, 0.4, and 0.5 mM). Which type of reversible enzyme inhibition is illustrated by inhibitor C? Since many of you do not have scanners or cameras to send me an e-mail image of your graphs,

Biochemistry
Biochemistry
9th Edition
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Chapter6: The Behavior Of Proteins: Enzymes
Section: Chapter Questions
Problem 60RE
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AutoSave 301-Enzyme Kinetics and Inhibition, Part 3 - Compatibility Mode - Word Search ff steve M SM File Home Insert Draw Design Layout References Mailings Review View Help E Share O Comments prepared five different concentrations of substrate (sucrose), and five different concentrations of inhibitor C (plus the control, with zero mM of inhibitor C). The following Table lists the inhibitor C concentrations [I], substrate concentrations [S], and resulting enzyme velocities (V.) for all six of these experiments: [I] O mM O mM O mM O mM O mM [S] Vo 1/[S] 1/ Vo 0.1 mM 0.333333333333 mM per minute 0.2 mM 0.50 0.3 mM 0.60 0.4 mM 0.666666666667 0.5 mM 0.714285714286 0.1 mM 0.1 mM 0.166666666667 0.1 mM 0.2 mM 0.25 0.1 mM 0.3 mM 0.30 0.1 mM 0.4 mM 0.333333333333 0.1 mM 0.5 mM 0.357142857143 0.20 mM 0.1 mM 0.111111111111 0.20 mM 0.2 mM 0.166666666667 0.20 mM 0.3 mM 0.20 0.20 mM 0.4 mM 0.222222222222 0.20 mM 0.5 mM 0.238095238095 0.3 mM 0.1 mM 0.083333333333 0.3 mM 0.2 mM 0.125 0.3 mM 0.3 mM 0.150 0.3 mM 0.4 mM 0.166666666667 0.3 mM 0.5 mM 0.178571428571 0.40 mM 0.1 mM 0.066666666667 0.40 mM 0.2 mM 0.10 0.40 mM 0.3 mM 0.120 0.40 mM 0.4 mM 0.133333333333 0.40 mM 0.5 mM 0.142857142857 0.5 mM 0.1 mM 0.055555555556 0.5 mM 0.2 mM 0.083333333333 0.5 mM 0.3 mM 0.10 0.5 mM 0.4 mM 0.111111111111 0.5 mM 0.5 mM 0.119047619048 Construct a Michaelis-Menten plot, and a Lineweaver-Burk plot, for all six of these experiments on the same graph (for each plot). Calculate the Vmax, the Km, and the slope (Km/Vmax) for the control (with [I] = 0 mM) and for each non-zero concentration of inhibitor C ([I] = 0.1, 0.2, 0.3, 0.4, and 0.5 mM). Which type of reversible enzyme inhibition is illustrated by inhibitor C? Since many of you do not have scanners or cameras to send me an e-mail image of your graphs, Page 1 of 2 389 words D, Focus 80%
Transcribed Image Text:AutoSave 301-Enzyme Kinetics and Inhibition, Part 3 - Compatibility Mode - Word Search ff steve M SM File Home Insert Draw Design Layout References Mailings Review View Help E Share O Comments prepared five different concentrations of substrate (sucrose), and five different concentrations of inhibitor C (plus the control, with zero mM of inhibitor C). The following Table lists the inhibitor C concentrations [I], substrate concentrations [S], and resulting enzyme velocities (V.) for all six of these experiments: [I] O mM O mM O mM O mM O mM [S] Vo 1/[S] 1/ Vo 0.1 mM 0.333333333333 mM per minute 0.2 mM 0.50 0.3 mM 0.60 0.4 mM 0.666666666667 0.5 mM 0.714285714286 0.1 mM 0.1 mM 0.166666666667 0.1 mM 0.2 mM 0.25 0.1 mM 0.3 mM 0.30 0.1 mM 0.4 mM 0.333333333333 0.1 mM 0.5 mM 0.357142857143 0.20 mM 0.1 mM 0.111111111111 0.20 mM 0.2 mM 0.166666666667 0.20 mM 0.3 mM 0.20 0.20 mM 0.4 mM 0.222222222222 0.20 mM 0.5 mM 0.238095238095 0.3 mM 0.1 mM 0.083333333333 0.3 mM 0.2 mM 0.125 0.3 mM 0.3 mM 0.150 0.3 mM 0.4 mM 0.166666666667 0.3 mM 0.5 mM 0.178571428571 0.40 mM 0.1 mM 0.066666666667 0.40 mM 0.2 mM 0.10 0.40 mM 0.3 mM 0.120 0.40 mM 0.4 mM 0.133333333333 0.40 mM 0.5 mM 0.142857142857 0.5 mM 0.1 mM 0.055555555556 0.5 mM 0.2 mM 0.083333333333 0.5 mM 0.3 mM 0.10 0.5 mM 0.4 mM 0.111111111111 0.5 mM 0.5 mM 0.119047619048 Construct a Michaelis-Menten plot, and a Lineweaver-Burk plot, for all six of these experiments on the same graph (for each plot). Calculate the Vmax, the Km, and the slope (Km/Vmax) for the control (with [I] = 0 mM) and for each non-zero concentration of inhibitor C ([I] = 0.1, 0.2, 0.3, 0.4, and 0.5 mM). Which type of reversible enzyme inhibition is illustrated by inhibitor C? Since many of you do not have scanners or cameras to send me an e-mail image of your graphs, Page 1 of 2 389 words D, Focus 80%
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