Using data from Table 1 plot a graph similar to Figure 4 in the manual that you will label as “Figure A - Bromophenol Blue Stock Solution Absorbance Spectrum”. Remember all graphs need x-y-axis labels and titles. Plot the graph of order 2 polynomial as the best fit curve. The best-fit curve should show the equation of the line, it does not need to pass through the origin (0,0

Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter20: Molecular Spectroscopy And Photochemistry
Section: Chapter Questions
Problem 6P
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Using data from Table 1 plot a graph similar to Figure 4 in the manual that you will label as “Figure A - Bromophenol Blue Stock Solution Absorbance Spectrum”. Remember all graphs need x-y-axis labels and titles. Plot the graph of order 2 polynomial as the best fit curve. The best-fit curve should show the equation of the line, it does not need to pass through the origin (0,0).

 

3. Constructing a Standard Curve
You will construct a standard curve
which shows the linear relationship
(according to Lambert-Beer's Law)
between absorbance and the
concentration for the serial dilution
of a given dye/pigment. With this
standard curve you can determine the
unknown concentration of a sample
containing the same dye/ pigment.
Figure 4 shows how the
concentration of the unknown is
determined from a standard curve.
To construct a standard curve you
need to make a series of solutions of
known concentrations through serial
dilutions.
Metric reminder: 1μL-0.001mL;
1mL=0.001L; 1mg = 0.001g
Absorbance
1.2
1
0.8
0.6
0.4
0.2
0
0
0.2
y = 1.155x
R² = 0.9297
0.4
0.6
Concentration (mg/ml)
Conc. of unknown
determined from
equation of the
best fit line
0.8
1
Figure 4 - Standard curve created from the absorbance data
of bromophenol blue solutions of known concentrations.
Based on the line plotted from these known solutions, the
concentration of the solution of unknown concentration can
be determined.
Transcribed Image Text:3. Constructing a Standard Curve You will construct a standard curve which shows the linear relationship (according to Lambert-Beer's Law) between absorbance and the concentration for the serial dilution of a given dye/pigment. With this standard curve you can determine the unknown concentration of a sample containing the same dye/ pigment. Figure 4 shows how the concentration of the unknown is determined from a standard curve. To construct a standard curve you need to make a series of solutions of known concentrations through serial dilutions. Metric reminder: 1μL-0.001mL; 1mL=0.001L; 1mg = 0.001g Absorbance 1.2 1 0.8 0.6 0.4 0.2 0 0 0.2 y = 1.155x R² = 0.9297 0.4 0.6 Concentration (mg/ml) Conc. of unknown determined from equation of the best fit line 0.8 1 Figure 4 - Standard curve created from the absorbance data of bromophenol blue solutions of known concentrations. Based on the line plotted from these known solutions, the concentration of the solution of unknown concentration can be determined.
Table 1 - Absorbance reading for Bromophenol
Blue Stock Solution.
Wavelength (nm)
475
500
525
550
575
600
625
650
675
Absorbance
0.013
0.014
0.066
0.142
0.272
0.206
0.160
0.033
-0.026
Copy and paste your graph from Table 1 here:
Answer these questions:
Q1) Wavelength of peak absorbance for Bromophenol
Blue is 575 n m
Q2) The units for absorbance is_NIA
2
Transcribed Image Text:Table 1 - Absorbance reading for Bromophenol Blue Stock Solution. Wavelength (nm) 475 500 525 550 575 600 625 650 675 Absorbance 0.013 0.014 0.066 0.142 0.272 0.206 0.160 0.033 -0.026 Copy and paste your graph from Table 1 here: Answer these questions: Q1) Wavelength of peak absorbance for Bromophenol Blue is 575 n m Q2) The units for absorbance is_NIA 2
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