Using the following absorbance data set, would you c Salt concentration Organism A Organism B 1% 0.477 0.008 3% 0.206 0.100 7% 0.031 0.311 11% 0.002 0.214
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- you did a serial dilution experiment to determine the CFU/table of the Equate Children's probiotics. Given the ingredient list shown, how many colonies would you expect to see on a plate if you plated 0.1 mL from the 10-7 dilution tube, and incubated aerobically at 37 ⁰C for several days?Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/La. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.
- You did a serial dilution experiment to determine the CFU/table of the Webbers' probiotics. Given the ingredient list shown, how many colonies would you expect to see on a plate if you plated 0.1 mL from the 10-6 dilution tube, and incubated aerobically at 37 ⁰C for several days?in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45Suppose you inoculated two 0.4% semi-solid agar deeps: one deep was inoculated with a motile organism and the other deep with a non-motile organism. You (in a stroke of genius) found a way to quantitatively analyze the deeps using a spectrophotometer. Which deep would have the highest absorbance and why?
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- a. Is there a microbe that could grow on a medium that contains onlythe following compounds dissolved in water: CaCO3, MgNO3,FeCl2, ZnSO4, and glucose? Defend your answer.b. A useful mnemonic device for keeping track of major elementsrequired by most organisms is: C HOPKINS Ca Fe, Na ClMgood. Read aloud, it becomes “See Hopkin’s Cafe, NaCl (salt)M good!” What does each letter stand for?How would I graph a scatterplot/ x-y plot of temperature and absorbance. Temperature is the independent variable (x-axis) and absorbance is the dependent variable(y-axis)FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?