Which of the ff. is carried out when sample has a low absorbance? Use a cuvette with shorter width. Use a cuvette with longer width. Use a quartz cuvette instead of ordinary glass. O Use ordinary glass cuvette instead of quartz.
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- Which statement(s) describe(s) a microplate reader?A. It is spectrophotometerB. It can read the absorbance of one sample with adjustable path lengthC. It can handle multiple samples in parallelD. It will do all measurement jobs without our supervision a. A & B b. A, B & C c. A, B, C & D d. A e. A & CA student performs a separation by TLC on a silica get plate that results in three spots. Ared spot is measured 23 mm away from the original sample spot, a blue spot is 42 mmaway, and a yellow spot is 49 mm away. The distance from the original spot to where thesolvent stopped is 55 mm.a) Calculate the Rf value for each component in the mixture. (Show your work and reportthe correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest tohighest polarity. Explain your answer.Which of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.
- Test solution Indicator thatcan be usedInitial colour ofthe solutionFinal colourof thesolution1. Lemon juice Blue litmus Red1. Tools that do not need to be brought when sampling is…a. thermometerb. Hygrometerc. PH meterd. DO meter 2. In the deposition method, one of the conditions that must be met is…a. The precipitate has a high solubility or solubility productb. The precipitate formed must be quite difficult to dissolvec. The precipitate formed does not have to be quite pured. Free precipitate formWhen loading samples onto a gel, why should expelling all the liquid in the tip be avoided? a)All the sample could be forced out from the well in which it was loaded. b)The extra liquid would end up in the buffer and this would distort the migration of the sample. c)The gel could tear and distort the migration of the sample. d)An air bubble could result which would distort the migration of the sample .
- You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equationWhat precautions should we follow when conducting experiment in Environmental lab? How can you determine chloride content in a waste water sample?Your spectrophotometer maxes out at an absorbance reading of 2. If you get a reading of 2, what could you do to your sample to get an accurate reading.
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperI do not understand the results of this experiment, explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088Design a serial dilution of a sample to achieve a A) Final dilution of 10-4 using 9.0 mL blanks B) Final dilution of 10-6 using 99 mL blanks C) Final dilution of 10-6 using 9.0 mL blanks