a)List and describe the purpose of each component of the restriction enzyme digest.

Q: How would restriction enzymes play a role in having an organismproduce a protein that it normally…

A: Restriction enzymes are specialized proteins produced by the bacteria that cut the DNA at a specific…

Q: The type of restriction enzymes that do not usually require ATP is : * О Туре ! О Туре I О Туре II O…

A: A restriction enzyme, also termed as a restriction endonuclease or restrictase, is an enzyme that…

Q: Define restriction point,

A: The cell cycle is the initial process that cells follow to prepare themselves for cell division.…

Q: What bacteria were used for the isolation of the three enzymes (HindIII, NdeI and PvuI)? What type…

A: HindIII  is isolated from Haemophilus influenzae . NdeI is an  isolated from Neisseria…

Q: ocedure to extract DNA through banana?

A: A nucleic acid called DNA is arranged into chromosomes. It has genetic data in it. Polynucleotide…

Q: Based on the restriction enzyme specificities given below, which will generate blunt ends?…

A: Restrictions enzyme or restrictions endonucleases is type of protein, produces bacteria that helps…

Q: What is the function of lysozyme in egg white?

A: Lysozyme is an enzyme ( glycoside hydrolase)that has bactericidal properties. It is present in human…

Q: Single and double digestion of plasmid pJC716 were performed using the restriction enzymes EcoRI and…

A: Plasmid is a double stranded circular dna molecule that is capable of replicating independently. It…

Q: What do restriction enzymes do

A: What do restriction enzymes do ?

Q: What is the job of the restriction enzyme?

A: In the year 1963 the two enzymes responsible for restricting the growth of bacteriophage in E. coli…

Q: Describe the role of restriction enzymes in the process of transformation.

A: Answer :- Restriction enzymes are utilized to cut the DNA of both the living being with the ideal…

Q: What is the purpose of bacterial transformation?

A: Transformation is the genetic alteration in a cell by direct uptake and incorporation of exogenous…

Q: Why are the restriction enzymes added last to the digestion mixtures?

A: The restriction enzyme is the end component added to a reaction to ensure that it is not exposed to…

Q: What does the plasmid contain the code for?

A: Plasmids are extrachromosomal autonomously replicating double-stranded circular DNA molecules…

Q: For sample 2, how many different size fragments will be generated after completing digestion with…

A: Introduction  Enzymes are the crucial biomolecules which assists in various biochemical reactions…

Q: a restriction enzyme is one that?

A: A restriction enzyme is a most important tool in recombinant technology. The alteration of the…

Q: After sequencing E.coli ROAR340 strain and using Silico Clermont Phylotyper tools show that is it…

A: Escherichia coli, also known as E. coli, is a gram-negative, facultative anaerobic, rod-shaped…

Q: Why is it necessary to load the same amount of protein for each sample during electrophoresis?

A: Electrophoresis can be described as the movement of particles (charged particles) in a gel or fluid.…

Q: What are the advantages and disadvantages of differential centrifugation?

A: differential as the name suggests is used to separate heterogenous solutions into their subsequent…

Q: Below are the events that occur when recombinant DNA procedure is performed. Identify and describe…

A: Genetic engineering is the field of biology which uses concepts like recombinant DNA technology, in…

Q: What uses of Type 1 Restriction Enzymes have if they seem to be so inaccurate?

A: Restriction enzymes are used to cut the DNA into fragments that are suitable for manipulation and…

Q: Describe ,A Hfr bacterium mates with an F- plasmid containing bacterium. What

A: PLASMIDS- It was discovered that maleness in bacteria is determined by a transmissible genetic…

Q: Define about restriction site ?

A: Recombinant DNA is made up of molecules of DNA from two different species that are inserted into a…

Q: What mechanisms account for restriction and modification?

A: Restriction-modification system is present in prokaryotic organisms like bacteria. Prokaryotic cells…

Q: In the bacterial transformation experiment, what is the primary purpose of using an…

A: Transformation experiment was given by Griffith. In bacteria, DNA can be transferred using…

Q: How to determine the location of restriction sites for different enzyme ?

A: Enzymes are proteins that are present in the body and help to speed up metabolism or other chemical…

Q: How do the following factors affect the activity of restriction enzymes? pH conditions Mg2+…

A: Restriction enzymes (restriction endonucleases) are the enzymes that can cleave at specific sites in…

Q: What uses do Type 1 restriction enzymes have if they seem to be so inaccurate

A: Restriction enzymes refer to the restriction endonuclease enzymes that are used to cut DNA…

Q: What is TFIIB ?

A: ANSWER:  Transcription factor II B (TFIIB) is a transcription factor that helps in the formation of…

Q: Who did discover restriction enzymes and how did they discover it? And what experiments did they use…

A: It is the one of the tools of recombinant DNA technology which is known as molecular scissors. Today…

Q: Which are the Ten Commonly Used Restriction Enzymes?

A: A restriction enzyme could be a macromolecule that acknowledges a particular, short nucleotide…

Q: What are the optimal conditions for EcoRV and Aval restriction enzymes?

A: Restriction enzymes are also known as molecular scissors since they are used in cutting specific…

Q: How many restriction sites does each enzyme have? Restriction Fragments generated Number of sites…

A:

Q: Does bacterial transformation occur in nature?

A: Transformation is the change in the genome of an organism. Transformation is one of the methods of…

Q: Define the concept of restriction-enzyme digestion ?

A: Enzymes are biocatalysts. These speed up biochemical reactions and are proteinaceous in nature.…

Q: What is TFIIF ?

A: Transcription regulatory molecules are the molecules or factors that regulate the transcription…

Q: How is a specific probe obtained?

A: Probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a…

Q: are restriction endonuclease enzymes named? Write examples.

A: Restriction enzymes break DNA at or near certain recognition sequences known as restriction sites,…

Q: What is the difference between the total and faecal coliform

A: MPN assay is the statistical, multi-stage test for determining the presence of bacteria in water,…

Q: What are the potential benefits and disadvantages of using bacteriophages in place of antibiotics?…

A: Compared to antibiotics, only a single phage is required to kill a single bacterium and so fewer…

Q: How do the following factors affect the activity of restriction enzymes? pH conditions Mg2+…

A: Restriction enzymes are those enzymes which cut the DNA at specific places so that they can be used…

Q: What would happen to a bacteria that lost a plasmid for antibiotic resistance?  Would it still be…

A: The term Antibiotic resistance  refers to the phenomenon where the bacteria develops a resistance…

Q: How Web site, Webcutter are used for generating restriction maps ?

A: Constructing some long-range restriction maps are dependent on making the use of restriction…

Q: How would you ascertain if your restriction experiment was successful?

A: Restriction digest of plasmid DNA is done using the naturally occurring restriction enzymes that…

Q: cytosine is deaminated. Describe the outcome of this deamination and explain in detail how E. coli…

A: DNA is made up of four bases two purines and two pyrimidines. Two purines are adenine and guanine.…

Q: Does bacterial transformation occur naturally?

A: Transformation is the change in the genome of an organism. Transformation is one of the methods of…

Q: a bacteriophage has been isolated from the growth chambers that has evolved the capability to the…

A: In the modern age of technological revolution, various different techniques of genetic engineering…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.