What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.

Q: What is a database? What types of information are stored within adatabase? Where does the…

A: The complete set of chromosomes present in the organism is known as the genome. Bioinformatics…

Q: Which of the following cloning vectors have the minimal features required for cloning ? Answer =

A: Answer :: Brief description about cloning Cloning is a process that allows scientists to create…

Q: Starting with a sample of RNA that contains the mRNA for theβ-globin gene, explain how you could…

A: DNA is the genetic material that carries genetic information in the form of coded nucleotide…

Q: What is an advantage of making a cDNA library rather than a genomic library?

A: DNA library or the gene library is the collection of DNA sequences from different organisms. These…

Q: Diagram a method for creating a cDNA library

A: cDNA library ( COMPLEMENTARY DNA) It is a combination of cloned cDNA fragments which is inserted…

Q: What can serve as footprints to identify genome sites that are or have been occupied by transposable…

A: A genome is a collection of an organism's genetic information.

Q: Why do geneticists studying eukaryotic organisms often construct cDNA libraries, whereas…

A: A cDNA (complementary deoxyribonucleic acid) library is a combination of cloned cDNA fragments…

Q: List three independent techniques you could use toidentify DNA sequences encoding human geneswithin…

A: A genome is referred to as genetic material of the organism that composed of DNA or RNA. The genome…

Q: A DNA library is a collection of clones, each containing a different fragment of DNA, inserted into…

A: According to the question, A DNA library is a collection of clones, each containing a different…

Q: Why is cDNA used for cloning?

A: Cloning is the production of organisms, which are genetically identical to their parents. These…

Q: How are probes used to screen DNA libraries? Explain how a synthetic probe can be prepared when the…

A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…

Q: What major advantage does cloning mammalian genes frommRNA or using synthetic genes have over PCR…

A: Cloning by using mRNA requires DNA with desirable genes obtained using restriction enzymes which are…

Q: If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a…

A: Transformation is the process in which a bacterium takes up DNA from the environment, that has been…

Q: What is a cDNA library, and for what purpose can it be used?

A: In genetics, the term cDNA library refers to the library containing the group of cloned copies of…

Q: What types of cells are used for cloning rDNA?

A: rDNA is called recombinant DNA which contains DNA from multiple sources. Recombinant DNA is it's the…

Q: What is RNA sequencing ? Why it is important ?

A: The biochemical material that is carried from the preceding generation to the succeeding generation…

Q: Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by…

A: Synthetic probe is fragment of DNA. to screen a DNA library RNA of variable length approximately 100…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: Although a number of different animals have been successfully cloned, the process of creating cloned…

A: A living organism, which is genetically identical to that of the other organism is known as a Clone.…

Q: You are trying to study the effects of Drug A on the expression of Gene X in a tumor sample (lane…

A: Reverse transcription enzyme chain reaction (RT-PCR) may be a variation of normal PCR that involves…

Q: What are the advantages of Next Generation Sequencing?

A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…

Q: What are the essential elements of a plasmid cloning vector? Discuss the importance of each element…

A: Essential elements of plasmid cloning vector  Presence of Origin of replication for autonomous…

Q: The plasmid cloning vector pBR322 is cleaved with the restriction endonuclease PstI. An isolated DNA…

A: The following is the DNA information received by gel electrophoresis:   In lanes 1 and 2, each…

Q: What are the main  differences between whole genome sequencing and whole exome sequencing?

A: whole genome sequencing is sequencing the entire genome of the organism, where as whole exome…

Q: Explain about preparation of cDNA libraries ?

A: cDNA (short for complementary deoxyribonucleic acid) is produced from messenger RNA in a process…

Q: "Complementary DNA (cDNA) libraries offer certain advantages over genomic libraries". Explain how ?

A: Introduction The cDNA library is a collection of mRNA segments cloned into independent vector…

Q: Consider the following human genetic diseases: hemophilia, Down syndrome, cystic fibrosis, and brain…

A: CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats) Cas genome editing technology…

Q: You have two samples you have to sequence: one is a cloned plasmid that you want to verify the…

A: Sequencing of DNA refers to the biochemical methods for determining the order of the nucleotide…

Q: Suppose that a human genomic library is prepared by exhaustive digestion of human DNA with the Eco…

A: The human genome project was started in the year 1990, the main objective of this genome project is;…

Q: Suppose that a human genomic library is prepared by exhaustive digestion of human DNA with the EcoRI…

A: No,  because most humans genes are much longer than 4kb.

Q: CRISPR One of the more recent advances in biotechnology is the development of the CRISPR gene…

A: Given information: The CRISPR technology is the most recent advancement in the field of…

Q: After Create a second cDNA strand complementary to the first. After the reaction is completed, the…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: In large-genome sequencing projects, the initial data usually reveal gaps where no sequence…

A: Primer walking or directed sequencing is a sequencing method for sequencing DNA fragments that are…

Q: CRISPR RNA (crRNA) in a bacterial cell: is a snippet of genetic material from a virus stored in a…

A: The CRISPR/Cas9 or clustered regularly interspaced short palindromic repeats and CRISPR-associated…

Q: Suppose you were interested in generating a PCR amplicon including the bracketed sequence below.…

A: PCR or Polymerase Chain Reaction is a specialized type of method which is used to obtain copies of…

Q: In your own words, describe the series of steps necessary to clone a gene.

A: Cloning is the process of producing genetically identical copies of an individual by natural or…

Q: List the steps to make a genomic library. What steps would change if you wanted to make a cDNA…

A: You have asked two questions. The first one has been answered. To get the answer for the second…

Q: What is an STS? How are STSs generated experimentally? What are the uses of STSs? Explain how a…

A: BASIC INFORMATION GENE MAPPING  This is a technique to identify the position of elements in the…

Q: PCR errors during library amplification are one possible source of false positive results. If an…

A: PCR is a polymerase chain reaction. This is a biotechnology used to amplify the DNA copies.

Q: Why is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or…

A: Two crucial components make up the CRISPR-Cas9 system, which modifies DNA. These include the Cas9…

Q: List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs…

A: Cloning vectors are small DNA pieces that are maintained stably in an organism. This vector can a…