Answered: What are the chief differences between…

What are the chief differences between the Ninhydrin and Bradford assays for amino acid quantification? Why would one technique be preferred over the other?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Related questions

Q: Calculate the cell potential (Eell ) for the reaction given below. 2Li(s) + 2F-(aq) → 2Li*(aq) +…

Q: Write the equation and write the correct IUPAC name of the product of the ff A.dehydration of…

A: The possible outcomes for the given reactions are alkene, carboxylic acid and tetrabromide…

Q: A series of eight absorbance measurements using an atomic absorption spectrophotometer are as…

A: Given,

Q: A dilute aqueous solution of CaC₂ contains 0.159 grams of solute per liter of solution. It is fully…

A: Formula : π = i MRT Where π is the Osmotic pressure iis the no.of ions…

Q: A reaction between liquid reactants takes place at 33.0 °C in a sealed, evacuated vessel with a…

Q: Consider the titration of 40.0 mL of 0.200 HClO4 by 0.100 M KOH. Calculate the pH of the resulting…

A: Given that - Volume of HClO4 = 40.0 mL Molarity of HClO4 = 0.200 M Molarity of KOH = 0.100 M…

Q: How much energy (in KJ) does it take to melt 25.0 grams of ice? ^Hfus = 6.02 kJ/mol ^Hvap = 44.0…

Q: 5. Calculate the 143ND/144N ratio of a basalt after it has interacted with seawater, given the…

A: Give in following question Nd isotopes intrect these isotopes with sea water the ratio of the Nd 143…

Q: C. Write the systematic name for the following compound according to Hantzsch- Widman Nomenclature…

Q: Propionic acid is provided (pKa - 4.0) to make a 0.8 M buffer at pH = 6.0. What is the ratio of…

Q: When 6.29 g of a certain nonvolatile solute is dissolved in 500 g of water, the freezing point of…

Q: Which equilibrium constant represents a reaction that favors the formation of the products to the…

A: ★ Answer : Correct option is : Keq = 1.0 × 108 ★ Solution :

Q: eases, the number of moles of NO(g) will... decrease increase stay the same 3.For a given…

Q: Van't Hoff equation estimates the Ws of a rue False

A: Osmotic pressure (Ψ)s is determined by using Van't Hoff equation, (Ψ)s = i×CRT

Q: The solubility of carbon dioxide in water at 25°C and 1 atm is 0.034 mol/L. What is its solubility…

A: We have to predict the solubility of the gas.

Q: what is the hybridization and geometry of the nitrogen atom

A: What is the hybridization and geometry of the nitrogen atom ?

Q: in this E2 reaction what is the major product

Q: Give a clear handwritten answer with explanation...give the IUPAC name given structure

Q: ओोण्ए अर कीलबील विगताग्यय ०ी "e १raत्तपतर क ते पक हि जेकव्यी बठार निठकरत कि ककर गण्लंमू begप वेनnय…

Q: What substance will be most soluble in carbon tetrachloride, CCI4? A Ethanol, C2H5OH 12 C KNO3 NaCl…

Q: rater, Nd

A: The basalt before alteration values were given. Estimated values of sea water can against the value…

Q: 1. Nitrogen Gas 2. Oxygen Gas 3. Fluorine Gas look for its critical parameters and calculate its…

Q: Furthermore, the following reaction occurs between O (formed either from the photochemical…

A: Propagation step is that step in which a radical is consumed and a new radical is formed. While…

Q: Your pure water boils at 100.00 C. You add some sugar into it and now it boils at 101.20C. What is…

A: Given, Boiling point of pure water, Tb° = 100.00 °C Boiling point of solution, Tb = 101.20 °C Kb…

Q: Given the reaction at equilibrium: X2(g) + 2 Y2(g) 2 XY2(g) + 80 kcal The equilibrium point will…

A: According to Le- Chatelier's principle when any disturbance is made on the equilibrium then it…

Q: Define cohesion and adhesion, and how they can predict capillary action.

A: Adhesion can be defined as the sticking tendency of particles of different types of material.…

Q: the product is given predict from what alkene reactant be made from, write the complete reaction on…

A: Given, the product of A. 2-methyl-2- hexanol B. 3-chloro-3-ethylpentane…

Q: Calculate the Gibbs Free Energy for the following cell. V or PS NaCl Half-Reaction E° (V) Cu2+ + 2e…

A: The electrode which has higher reduction potential acts as a cathode and the electrode with lower…

Q: In a lab the base solution of Potassium Iodide is 0.225L at 0.80M. if a student adds enough water to…

A: The molarity decreases upon dilution .So for the calculation of new concentration ie after dilution…

Q: Practice Exercise Calculate the pH at the equivalence point in the titration of 50 mL of 0.10 M…

Q: (a) You are required to produce a BCC iron structure that will allow no more than 50 g of hydrogen…

A: (a)

Q: Calculate the solubility of MgF2 (MW = 62.3 g/mol) in water at 25 °C if its Ksp is 7.4 x 10-11.? No…

A: From ICE TABLE-

Q: खो फंक प्वो 1 +ैर Pिब्डब कीपट बैकपलेपरर टमतु- तH ( तCम5 ) C H+- - CH-्तभ० फर्वठकुन्द वकल वडांटेबनणल…

A: Here we are required to mechanism for the reaction

Q: 8. What products do you expect from the reaction of the following ethers with HI? (a) (b) CH3 CH3 ?…

A: Q8. In presence of HI , the ether gets protonated first and then if there is possibility of…

Q: 1. Such as in the interior of giant planets, it is argued that water exists as ionic water in which…

A: Answer: Water is a molecule with a bent shape. In this molecule oxygen is the central atom and two…

Q: Calculate the amount of heat absorbed (in Joules) by a substance when the temperature changes from…

Q: Nal acetone

Q: Subtractive color mixing is: O colored light is combined with colored light O colorants are combined…

A: Processes of combining colored light is called: additive color mixing. When colorants are combined…

Q: Calculate the work (J) of the system when the ideal gas expands at constant pressure of 2.480 atm…

A: Work done at constant pressure is given by W = -P∆V ........(1) W = work done = ? P = pressure =…

Q: For some medical procedures, doctors cool the patient's body before beginning. Following the…

A: ✓Normal functioning of human body is based on homeostasis. ✓Homeostatis is the process by which the…

Q: If CF2CI2 exists in the stratosphere, which radicals will be formed when it is irradiated? What…

A: Because carbon chlorine bond energy is smaller than carbon fluorine bond energy so it is easier to…

Q: Calculate the solubility of MgF2 (MW = 62.3 g/mol) in water at 25 °C if its Ksp is 7.4 x 10-11.?

Q: A new material crystallizes in the face-centered cubic unit cell. The molar mass of the material is…

Q: Using "like dissolves like" solubility rule, determine if the substance is more soluble in H20 or in…

Q: What is the predicted product of the reaction shown? 1. NaOCH,CH3 2. H3O* II OH IV

A: The close observation of reactants and reagents indicates that, we can expect a cyclized ketone as…

Q: Write the equation and write the correct IUPAC name of the product of the following A. Bromination…

Q: please give proper explanation

A: The stretch of C=O bond depends on the strength of the bond. If strength of the bond less, it will…

Q: give the meaning and describe metabolic process

A: “Metabolism refers to a series of chemical reactions that occur in a living organism to sustain…

Q: The equilibrium constant is given for one of the reactions below. Determine the value of the missing…

A: 1. Equllibrium constant is product of molar concentration of products each raise to power equal to…

Q: lus engine.html?ClassID=D120355934# ent Orie... Calculate the Gibbs Free Energy for the following…

Question

What are the chief differences between the
Ninhydrin and Bradford assays for amino
acid quantification? Why would one
technique be preferred over the other?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

Look up the pKa values for trimethylamine, dimethylamine, ethylamine, and ammonia. Predict the order of elution of these compounds from a cation-exchange column eluted with a gradient of increasing pH, beginning at pH 7.
Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram? Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?
A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?
what is the purpose of hydroxylamine hydrochloride in the spectrophotometic determination of iron in FAS (ferrous ammonium sulfate or Mohr's salt)?
Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse chromatography? explain
Why does a molecularly imprinted polymer selectively bind a desired analyte?
A student has a purple dye mixture on which she performs a TLC experiment with a silica gel plate and acetone as the solvent. She obtains a blue spot with an R of 0.89 and a red spot with an R of 0.45. Explain the significance of her data
Explain the differences observed in the Tg and Tm values for each pair of polymers: (a) polyester A and PET; (b) polyester A and nylon 6,6. (c) How would you expect the Tm value for Kevlar (Section 30.6A) to compare with the Tm value for nylon 6,6? Explain your prediction.
A student has a green dye mixture on which she performs a TLC experiment with a silica gel plate and acetone as the solvent. She obtains a yellow spot with an Rf of 0.75 and a teal spot with an Rf of 0.45. Explain the significance of her data.
The relative electrophoretic mobilities of a 30-kDa protein and a 92-kDa protein used as standards on an SDS– polyacrylamide gel are 0.80 and 0.41, respectively. What is the apparent mass of a protein having a mobility of 0.62 on this gel?
Biuret Assay accurately quantify protein concentration within the range of 5-150 mg/mL. JUSTIFY THE STATEMENT.
The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67