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- An E. coli colony grew on minimal medium supplemented with arginine and leucine. However, bacteria from this colony are unable to grow and form colonies on minimal medium supplemented with arginine and methionine. What is the genotype of the bacteria in this E. coli colony?"what is the reason for forming mucoid colonies in some types of bacteria?"Why might bacteria use quorum sensing to regulate genes needed for virulence? How might this reason be related to the rationale behind using quorum sensing to establish a symbiotic relationship?
- What are the implications of mutation to Pseudomonas aeruginosa as a pathogenic strain?why is peptidoglycan synthesis inportant in antibiotic activity? why do antibiotics not interfere with cell wall synthesis in the host cells?Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.
- Give two reasons why E. coli is naturally resistant to antibiotics like Penicillin?Why are gram-negative bacteria more resistant than gram-positive bacteria to the cytoplasmic target antibiotics?How would someone design an experiment to test the role of azithromycin on quorum sensing in Pseudomonas aeruginosa?
- How is host cell lysis triggered after bacteriophage invasion? What are the outcomes to bacterial host physiology if the bacteriophage undergoes the lysogenic cycle?Why are certain anti-bacterial agents more effective at killing bacteria?Why the synergy test results demonstrated that Penicillin G and Gentamicin did not act synergistically against the Streptococcus group G strain?