what are the principle chemical reaction involved in each test?
Q: Compare and contrast Benedict’s Test, Reagent Test, and Iodine Test in terms of materials used
A: Benedict's Test is used to test for simple carbohydrates. The Benedict's test identifies reducing…
Q: The reaction in Fohl’s test will cause the formation of what compound? What type of reaction happens…
A: Fohl's test is the biochemical test of proteins for the detection of presence of Aminoacids with…
Q: What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is…
A: Methyl red test This test usually aims to differentiate the facultative anaerobes enteric bacteria.…
Q: Table 1, Effect of enzyme concentration Amount of lodine Test Results (color) Amylase Enzyme 0 drops…
A: There are various tests used to detect the presence of polysaccharides including disaccharides and…
Q: What amino acid found in proteins causes the response in the Hopkins- Cole test?
A: Amino acids are the simple basic units that are used to make up all the proteins inside the cell.…
Q: Give the principle involved in the ninhydrin test. What is its purpose?
A: The principle involved in the ninhydrin test is that the two molecules of ninhydrin react with the…
Q: Explain the chemical basis (reaction) of Millon’s test. (Generic reaction and explanation) What is…
A: Proteins are made up of amino acids joined together by peptide bond. Proteins can be estimated by…
Q: What are the enzyme assays for
A: Enzymes are biocatalyst that increases the speed of reaction by lowering the activation energy.…
Q: What is the chemical basis of Molisch test?
A: Molisch test is a colorimetric method that is used to detect the presence of carbohydrates in a test…
Q: In urea hydrolysis what emd product is specifically being tested for in this test? Is the final ph…
A: The general formula or urea is NH2CONH2. Urea breakdown requires the enzyme urease. It is primarily…
Q: What other test(s) are used in parallel to Benedict’s test?
A: Reducing sugar: a. Carbohydrates that contain free aldehyde or ketone group and are in hemiacetal or…
Q: Which is the better reagent for the clinical test between Fehling's solution and Benedict's…
A: The reducing sugars get oxidized by reducing some other component. This reducing property of these…
Q: Why did the blank used in experimental procedures contain substrate, buffer and water?
A: A blank or blank determination is an analysis without a sample (Enzyme). Any enzyme assay requires a…
Q: What is a Goldbeater’s test? What is the principle involved?
A: Tannins are complex, nitrogen-free polyphenols found in plant tissues. Plants produce tannins to…
Q: What are the disadvantages of biochemical test?
A: Biochemical tests refer to the test done to identify microorganisms. These tests are widely done in…
Q: What is the use of heat in Seliwanoff’s, Benedict’s and Barfoed’s tests?
A: The Seliwanhoff's, Benedicts's, and Barfoed's tests can qualitatively identify and distinguish among…
Q: How did the Hershey–Chase “blender experiments” confirmGriffith’s results?
A: Based on the Griffith experiment's observations, he concluded that genetic material is the…
Q: What is the purpose of the test tube containing distilled water in each test? Why is glucose called…
A: 1- The purpose of using distilled water as one of our test substances is that in a way it controls…
Q: When conducting the Triple Sugar Iron Test, what is the result of the bacteria Staphylococcus…
A: Triple Sugar Iron Agar test is a test which has three sugar (Lactose, Sucrose, and Glucose) and also…
Q: Give at least two (2) chemical tests for amino acids/ proteins other than what were performed in…
A: There are several other tests to determine properties of proteins and amino acids present in a…
Q: In Seliwanoff’s test, what is the function of resorcinol? What is the aim of using a strong acid?
A: Seliwanoff's test is a qualitative test for carbohydrates. Seliwanoff's test helps to differentiate…
Q: What is Ames test ?
A: Ames Test : It was devised by a scientist 'Bruce Ames ' . It is also known as Salmonella…
Q: Please explain the theoretical background (positive color reactions and what is being detected)…
A: Proteins are the polymers of amino acids. The amino acids are joined together by the peptide bonds.…
Q: Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to…
A: Benedict's test is used to test for simple carbohydrates. Benedict's test identifies reducing…
Q: What is the purpose of the sodium thiosulphate in the testing of treated water?
A: Testing of wastewater and treated water both demand a standardized process to be followed to obtain…
Q: If we want to observe the following reaction, what must we add to a test tube? Select all that…
A: A chemical reaction is defined as the interaction between different molecules.
Q: The arrowhead shaped zone shows what reaction? How do you set up the test for this reaction? For…
A: INTRODUCTION Clostridium perfringens is a normal bactetia seen in large intestine of humans and…
Q: Wintrobe from Westergreen regarding accuracy of the test in a tabulated order.
A: Both methods are used for estimating E.S.R rate or the erythrocyte sedimentation rate. Most of the…
Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…
A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…
Q: What is the principlels of in-dose test?
A: During the development of drugs, the efficacy of a new drug is typically determined by showing its…
Q: why would you perform an indole test?
A: The inside test is a qualitative approach of the biochemical analysis of bacteria that determine…
Q: State the principle that underlies the following biochemical tests: a) Methyl red test b)…
A: (a) Methyl Red Test Principle These bacteria initially metabolize glucose to pyruvic acid, which is…
Q: What is meant by the term plating efficiency?
A: The cells are cultured over the plate at the particular growth conditions within a particular time…
Q: What are the colour changes occur to the reducing sugars after benedict test is performed? Please…
A: Reducing sugar is a sugar that is capable to act as a reducing agent. Reducing sugars are the ones…
Q: Can galactric acid test/mucic acid test be used to determine the presence of milk in milk-chocolate…
A: A nutrient-rich white fluid secreted by the mammary glands of the mammals is referred to as milk.…
Q: What is the main purpose of Huble's Test? How does it differ/similar to Unsaturation Test? How do…
A: Lipids are a group of compounds that are oily or greasy in nature. They consist of alcohol generally…
Q: cal is utilized as the reagent known as VP-B?
A: The Voges-Proskauer (VP) test is generally used to test if an organism produces acetyl methyl…
Q: what is the purpose and objectives on doing nitrious acid test?
A: Amines are the compounds and functional groups having a basic nitrogen atom with a lone pair of…
Q: list the reagents used in the IMViC tests and what they detect
A: IMViC is a series of tests that include the indole test, methyl red test, Voges-Proskauer test, and…
Q: WHAT IS THE IMPORTANCE/BENEFITS OF BENEDICT'S TEST IN TEST FOR REDUCING SUGAR?
A: BENEDICT'S TEST is a test used to identify the sugars (usually monosaccharides and some…
Q: what are the Amino Acids that test positive for Ninhydrin test? what is the principle of detection?
A: All the alpha amino acids test positive for ninhydrin test.
Q: What is similar about this test and the TSIA test?
A: SIM medium is used to check different parameters such as Sulphur Reduction, Indole Production,…
Q: What is the purpose of Biuret’s test? In biuret’s test, does the color in each test tube containing…
A: There are different biomolecules, including carbohydrates, protein, lipids, etc., are present. The…
Q: He then ran the sample on an agarose gel and observed a strong band at approximately 400bp. What…
A: The band represent a small piece of DNA that was cut with restriction endonuclease and then…
Q: For the nitrate reduction test, complete the table below Reaction Gas evolution (Yes/No) Color…
A: Nitrate Reduction Test -- Introduction -- Oxygen is present in the atmosphere as O2 which function…
Q: What amino acid present in proteins is responsible for the reaction in Hopkins- Cole test ?
A: Amino acids are the monomer of the protein. Each amino acid has a carboxyl group, an amino group, a…
Q: What is Gunning’s Test?
A: Quantitative assays are assays that give accurate and exact numeric quantitative measure of the…
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
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- Test tube Ascorbic acid stock (20 μg/mL) Citrate buffer Final Concentrations 1 0 μL 1000 μL 0.0 μg/mL 2 125 μL 875 μL 2.5 μg/mL 3 250 μL 750 μL 5.0 μg/mL 4 500 μL 500 μL 10.0 μg/mL 5 750 μL 250 μL 15.0 μg/mL 6 1000 μL 0 μL 20.0 μg/mL 7 1 mL of the fresh orange juice (20x diluted) 8 1 mL of the heated orange juice (20x diluted) 9 1 mL of the pre-citrus urine sample (5x diluted) 10 1 mL of the post-citrus urine sample (5x diluted) Plot a graph in which y-axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors. 1. StandardCurve Sample (μg) Absorbance (520 nm) 0.0 0.621 2.5…Procedure:1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva toeach tube. Mix thoroughly.2. Place the first tube in ice water, the 2ndtube leave at room temperature, the 3rdtube in 40°C , the 4thtube at 60°Cwater bath and the 5thtube boil for 2minutes..3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4thtubeallows to stand for 30 minutes after heating for 2 minutes.4. Test the contents of each tube with iodine and benedict’s tests.Calculate the amount of BSA you will add to the tube to get the concentrations listed on the chart. Your stock tube is 2mg/mL. The amount of water will be the amount to bring your total volume up to 100uL. Standard Concentration BSA H2O Total volume A 200 ug/mL 100uL B 150 ug/mL 100uL C 100 ug/mL 100uL D 50 ug/mL 100uL E 20 ug/mL 100uL F 10 ug/mL 100uL G 5 ug/mL 100uL H 0 ug/mL 0 uL 100uL 100uL
- the docotor oreder oxacillin 650 mg every 6 hours the instructions on the 2 gram vial states to reconstitute with powder with 11.5mL of sterile water for injection, yuelding 220mg/1.5mL how many mL will you administerWhy an equal volume of air is injected when performing self-saleability test for rubber closure of a vial?11. ● Possible methods for the quantitative determination of sodium bicarbonate for injection are: A) Acidimetry B) Alkalimetry C) Complexometry D) Iodometry E) Refractometry ● Calcium chloride is available in dosage forms: A) Powder for preparation of injections B) Solution for injection 10% in ampoules C) Tablets of 0.3 g D) Solution for injection 25% in ampoules E) Dry dosed powder
- Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?Define D-dimer test and how to interpret the test. The reference range is in the table below Test Result (reference range) D-dimer, ng/mL FEU > 4000 (< 500)
- Order: Furosemide (Lasix) 30 mg, PO, bidAvailable: Furosemide 20 mg/2 mL How many milliliters of furosemide will you give per dose? __ mL Round to the nearest tenth.The patient is ordered 150,000 units of Pfizerpen. Using 10mL of diluent, how many mL(s) will you administer? If rounding is requiredm, round to the nearest tenth. (Image for question is attached for more information)Appropriate pippetor for measuring 10ul of liquid is: 200 1000 20