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- What diagnostic tests will benefit from collecting both a blood sample and a urine sample from a patient?
- What diagnostic tests will benefit from collecting both a blood sample and amniotic fluid from a patient?
- In Clinical Chemistry, are enzymatic methods for determining glucose, cholesterol, and uric acid more preferred than non-enzymatic methods? Why or why not?
- In
Microbiology , are there technologies that can help make Bacterial Culture and Sensitivity done faster? Provide evidence of use in the Philippines.
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- In clinical chemistry, are enzymatic methods for determining glucose, cholesterol, and uric acid more preferred than non-enzymatic methods? Why or why not? In microbiology, are there techniques that can help make Bacterial Culture and Sensitivity done faster? Provide evidence of use in the Philippines.In Clinical Chemistry, are enzymatic methods for determining glucose, cholesterol and uric acid more preferred than non-enzymatic methods? Why or why not? In Microbiology, are there technologies that can help make Bacterial Culture and Sensitivity done faster? Provide evidence of use in our country.Explain why it is important to follow proper precautions and use aseptic technique when working with clinical samples. Why do you think that samples from urine should be tested within 2 hours of collection, or if not possible, that the sample should be refrigerated?
- in clinical chemistry, are enzymatic methods for determining glucose, cholesterol and uric acid more preferred than non-enzymatic methods? in microbiology, are there technologies that can help make bacterial culture and sensitivity done faster?The Benzodiazepines test device is a qualitative, preliminary test which would be able to detect whether Benzodiazepines are present in the urine specimen at the cut-off concentration of 300 ng/mL. Is it possible to have a urine specimen which was interpreted as POSITIVE using the Benzodiazepines test device to be interpreted as NEGATIVE by Gas Chromatography/Mass Spectrometry? Justify your answer. A study was conducted by the manufacturer of the benzodiazepines test device to determine whether some compounds would have cross-reactivity at levels of 100µg/mL. List down examples of non cross-reacting compoundsMatch the following experimental methods with their specialty procedures and objectives. autoradiography use of green fluorescent protein (GFP) fractional centrifugation use of genetic mutants A. silencing genes means inhibition of the expression of a protein B. fluorescing GFP protein is fused with a protein tracked in the cell C. biochemical processes are visualized using radiolabeled materials D. tissue samples homogenized at varying centrifugal force leads to isolation of desired cellular fraction
- Briefly comment on the use of alkaline phosphatase assays as a diagnostic aid for a single named common medical condition.You perform an ELISA to test hospital soap samples for contamination with Pseudomonas aeruginosa. Other than the different molecule of interest, this ELISA works exactly like the one you saw in the video. You run the soap sample, a negative control, and a positive control, all in duplicate. How would you interpret the following results? Laboratory Notebook: ELISA – Pseudomonas aeruginosa Well Contents Observation at 15 min Result 1 Negative control 2 Soap sample 3 Positive control 4 Negative control 5 Soap sample 6 Positive control 7 Empty well 8 Empty well The soap is POSITIVE for the Pseudomonas aeruginosa antigen. You cannot interpret the soap data, because the NEGATIVE control didn’t work. The soap is NEGATIVE for the Pseudomonas aeruginosa antigen. You cannot interpret the soap data, because the POSITIVE control didn’t work.In the “sandwich” ELISA technique, what is the function of the capture antibody? In the “sandwich” ELISA technique, what is the function of the capture antibody? To link an enzyme and the molecule of interest To convert a substrate from colorless to blue To block any “open spaces” on the well before adding the samples that potentially contain the molecule of interest To concentrate the molecule of interest
- Why do some production of biomedical products do not contain animal serum andprotein?Enzyme chymotrypsin (enzyme digest) Samples: - 1 & 5- Standard Protein (Ladder) - 2 & 6- UNCUT (tube #1) - 3 & 7- 5 mins (tube #2) - 4 & 8- 30 mins (tube #3) Human and Bovine (Left) Chicken and Pigeon (Right) In this experiment we will be comparing the structural similarity of serum albumin from several different vertebrate species and thus examining how closely related the proteins from these species are to each other. These proteins have similar molecular weights. We will first cut the proteins with the enzyme chymotrypsin (enzyme digest). We will then separate the fragments based on their relative sizes through gel electrophoresis so that we can compare the fragments generated by the enzyme digest for the different species. Write the hypothesis and prediction for the experiment.Search for the principles and the uses of the following methods for protein measurements and provide an image that will serve as your visual reference. Serum Protein Electrophoresis Isoelectric Focusing Ion Exchange Chromatography Turbidimetry Solvent Fractionation