Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
What does site-directed mutagenesis allow you to do
that normal mutagenesis does not?
The site-directed mutagenesis (SDM) method allows us to create specific, targeted changes in double-stranded plasmid DNA. There are certain reasons to make specific DNA alterations (insertions, deletions, and substitutions), including:
- In order to study the changes in protein activity that occur as a result of DNA manipulation.
- We have to select or screen for mutations (at the DNA, RNA, or protein level) that consist of the desired property
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