How could site-directed mutagenesis be used to investigate the kinetic properties of an enzyme?

Q: What is a simple way of making a knockout mouse in terms of DNA recombinant technology?

A: A knockout mouse, or knock-out mouse, is a genetically modified mouse in which researchers have…

Q: How can the binding assay approach be utilized to match coding triplets with their amino acid…

A: Live cells use the genetic code to turn information encoded in genetic material (DNA or mRNA codon…

Q: how to generate specific mutations in anyE. coli gene by gene targeting.

A: Transgenic organisms or the genetically modified organism contain the gene(s) of different organism…

Q: Describe how the Ames test screens for potential environmental mutagens. Why is it thought that a…

A: Ames test is a biological assay to estimate the ability of a chemical compound to cause mutation.…

Q: Why was cDNA and not genomic DNA used in the commercial cloning of the human insulin gene?

A: The cDNA is synthesized from ssRNA or microRNA in a reaction. This reaction is catalyzed by the…

Q: Does use of nucleic acid probes depend on gene expression?

A: Introduction: Nucleic acid hybridization or nucleic acid probe are used to determine specific gene…

Q: could Nirenberg and Matthaei have substituted RNA polymerase instead of polynucleotide phosphorylase…

A: The scientist Marshall Nirenberg and Heinrich J. Matthaei designed an experiment to elucidate the…

Q: Can exonuclease be used while producing a recombinant DNA molecule?

A: Introduction: Genetic engineering can be defined as a method to use recombinant DNA or rDNA…

Q: what is thermal stability and explain how the thermal stability of named proteins were improved…

A: Proteins are highly, complex molecules that perform numerous important functions in the body. They…

Q: Briefly explain the frameshift mutation ?

A: Mutation can be defined as the change that occurs in the DNA sequence which is caused by mistake or…

Q: What is a chimera and how is it different from a “true transgenic animal”?

A: A chimera is an single organism with DNA of two or three different types in it. For example during…

Q: How can the Ames test distinguish mutagens that cause small insertions/deletions from those that…

A: *Ames test  a biological assay to find the mutagenic potential of an chemical compound which…

Q: How can a tRNA be engineered to encode for a syntheticamino acid?

A: The standard genetic code features 20 natural amino acids which are the units of proteins. They are…

Q: What are mutagenicagents?

A: Mutagen is an agent that may be a physical or chemical which changes the genetic material.

Q: ld telomerase inhibitors be used to treat cancer?

A: Telomerase, also known as  terminal transferase can be described  as the  ribonucleoprotein that…

Q: What is an operon? Explain an inducible operon.

A: In genetics, an operon is a functioning unit of DNA containing a cluster of genes under the control…

Q: What are some advantages and disadvantages of using mathematical models to study biological…

A: An operon is defined as a group of genes that has a common promoter and regulator and also…

Q: Briefly describe how retron gene editing works?

A: Precise genome editing is a potential technique for discovering causative genetic variations and…

Q: What is mean by RNA editing ?

A: RNA is a nucleic acid present in the cells of living beings.

Q: Why are cDNA libraries desirable for the expression of eukaryotic genes in prokaryotes?

A: In cDNA library ("c" in cDNA stands for "complementary"). It is a series of cloned diagnostic DNA…

Q: What does site-directed mutagenesis allow you to dothat normal mutagenesis does not?

A: The site-directed mutagenesis (SDM)  method allows us to create specific, targeted changes in…

Q: How can site-directed mutagenesis be useful to enzymologists?

A: Mutagenesis is the biological process of changing genetic information or DNA of an organism and that…

Q: How does a mutagen induce mutation ?explain with examples?

A: The DNA is a genetic material- a polynucleotide chain made up of the long chain of A, T, G, and C.…

Q: What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.

A: Genetic engineering is a process through which the desired gene of interest is introduced into the…

Q: discuss using named examples some of the disadvantages of protein engineering using site directed…

A: Protein engineering the process used in molecular biology laboratories to engineer or make…

Q: How DNA changes occur during spontaneous mutagenesis ?

A: Mutations are changes in the sequence of DNA. Mutations can happen as a result of DNA copying errors…

Q: How can we identify histone modifications?

A: Histones are the class of proteins which are associated with the DNA in the nucleus and aids in the…

Q: Why do gene targeting and mutagenesis screening in mice have potential benefits for humans?

A: Mice and humans have undoubtedly had the longest relationship among mammals and mouse had a…

Q: Explain the importance of exonuclease ?

A: Enzymes are proteins that aid in the speeding up of the bodies' metabolism, or chemical reactions.…

Q: Describe the cloning strategies that are mediated by homopolymers tailing, linkers and adaptors. How…

A: The cloning has the ability to produce a duplication of any biological cell by modified genetically.…

Q: Compare the following techniques which are used to investigate the active site of an enzyme: 5.1use…

A: Enzymes are proteins that act as biological catalysts. They are basically known to accelerate the…

Q: What is Central Dogma of Molecular Biology and how this concept is used in the development of…

A: Genetically modified organisms are those that have been modified by humans in order to show those…

Q: Why aren’t primary rRNA transcripts present in wild-type E. coli?

A: E.coli is a gram negative, faculatative anaerobic, rod-shaped, coliform bacterium of the genus…

Q: What is the role of enzyme “Ligase” in genetic Engineering?

A: Recombinant DNA technology is not a simple process. It aims at isolating desired DNA sequences from…

Q: What is Gene therapy – Illustrate using example of Adenosine deaminase deficiency?

A: Genetic engineering is a process through which the desired gene of interest is introduced into the…

Q: What is an operon? Describe the functions of regulators, promoters,and operators.

A: The structure responsible for the regulation of genes in order to synthesize the associated proteins…

Q: Transposable elements alter the genome by making what changes to genes?

A: Transposition may be related to replication, recombination, and repair. Insertion of transposable…

Q: What is the purpose of hydrolyzing the RNA before conducting biochemical tests?

A: Introduction: RNA is a type of nucleic acids that is a genetic material in some viruses.

Q: How might such a “competition assay” be used to determine the biological glycosaminoglycan that…

A: Introduction Glycosaminoglycans (GAGs) are negatively charged, unbranched heteropolysaccharides…

Q: Explain how site-directed mutagenesis can be used to produce an altered protein in bacterial cells.

A: Site-directed mutagenesis is a molecular biology technique that is used to make specific and…

Q: What is the purpose of transgenesis?

A: Genetic engineering is the process of using recombinant DNA (rDNA) technology to alter the genetic…

Q: Briefly explain how site-directed mutagenesis is carried out.

A: Site-directed mutagenesis (SDM) is a method to create specific, targeted changes in double stranded…

Q: using example what is a degenerate primer? with the aid of diagrams discuss how degenerate primers…

A: A degenerate primer is defined as: "A mix of oligonucleotide sequences in which some positions…

Q: What does gene shuffling mean, how is it done? What is it used for? What advantages does it have in…

A: During the meiosis cell division at prophase I, a pairing of homologous chromosomes takes place at…

Q: discuss using named examples some of the advantages and disadvantages of protein engineering using…

A: Protein engineering refers to the process of altering the amino acid sequence of a protein. The is…

Q: What is a frameshift error? Does the binding of methionine tRNA to the small subunit help reduce…

A: Answer: Introduction: Mutation- These are the random heritable changes that occurs in the DNA…

Q: Why is it practical to test carcinogens by a mutagenesis assay?

A: Carcinogens are the cancer causing substances. Cancer is an uncontrolled division of cell that has a…