You identify a proflavin-generated allele of a genethat produces a 110-amino acid polypeptide ratherthan the usual 157-amino acid protein. After subjectingthis mutant allele to extensive proflavin mutagenesis,you are able to find a number of intragenic suppressorslocated in the part of the gene between the sequencesencoding the N terminus of the protein and the originalmutation, but no suppressors located in the regionbetween the original mutation and the sequencesencoding the usual C terminus of the protein. Whydo you think this is the case?

Question

You identify a proflavin-generated allele of a gene
that produces a 110-amino acid polypeptide rather
than the usual 157-amino acid protein. After subjecting
this mutant allele to extensive proflavin mutagenesis,
you are able to find a number of intragenic suppressors
located in the part of the gene between the sequences
encoding the N terminus of the protein and the original
mutation, but no suppressors located in the region
between the original mutation and the sequences
encoding the usual C terminus of the protein. Why
do you think this is the case?

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