What is the charge of each amino acid residue in the peptide chain and what is the net charge on the whole protein at pH = 4 (Use the provided table)?KGLpka ValueAlpha Carboxy |+Alpha Amino Side ChainAmino AcidNameGlycineAlanineValine2.349.602.349.692.329.62Leucine2.369.60Non-Polar Amino Acids IsoleucineMethioninePhenylalanineTryptophan2.369.682.289.211.839.132.389.39Proline1.9910.60SerineThreonineCysteineTyrosineAsparagineGlutamine2.219.152.639.101.7110.788.33Polar Amino Acids2.29.1110.072.028.842.179.13Aspartic AcidGlutamic AcidLysineArginine2.099.823.86Acidic Amino Acids2.199.674.252.188.9510.79Basic Amino acids2.179.0412.48Histidine1.829.176.04

The peptide KGL contains the following amino acids lysine glycine leucine

Answered: What is the charge of each amino acid…

What is the charge of each amino acid residue in the peptide chain and what is the net charge on the whole protein at pH = 4 (Use the provided table)? KGL

Chemistry: Principles and Reactions

8th Edition

ISBN:9781305079373

Author:William L. Masterton, Cecile N. Hurley

Publisher:William L. Masterton, Cecile N. Hurley

Chapter23: Organic Polymers, Natural And Synthetic

Section: Chapter Questions

Problem 44QAP: A 1.00-mg sample of a pure protein yielded on hydrolysis 0.0165 mg of leucine and 0.0248 mg of...

See similar textbooks

Related questions

Q: A typical amino acid with one amino group and one carboxylicacid group, such as serine, can exist in…

A: At lower pH that means in acidic conditions the carboxylate ion takes up proton and exists as…

Q: At pH 7.4, the pH of blood plasma, do the majority of protein-derived amino acids bear a net…

A: Concept introduction: Isoelectric point (pI) is defined as pH at which the protein has no net…

Q: Consider the following peptide : Phe – Glu – Ser – Met and Val – Trp – Cys – Leu. Do these peptides…

Q: Explain which amino acids are acidic, which are basic, and which are neutral. Use theisoelectric…

A: Amino acids: At isoelectric point, proteins are neutral.

Q: amino acid will elute first

A: For cation exchange column, pH is kept slightly lower in comparison to pI value so that the…

Q: d. When 99% of glycine is in its -NH3* form, what is the pH of solution due to it's amino group?…

A: 99% of glycine is in its -NH3+ form, so we have

Q: If you were to design a small peptide with a large net negative charge at physiological pH, which…

A: A question about biochemistry, which is to be accomplished.

Q: In the dreaded Zephyer galaxy there is a race of Fairies that have the ability to change their body…

Q: Consider an amino acid (A) with no ionizable side chains, and call the three species involved in the…

A: Using Hendesen-Hasselbalck equation pH = pKa + log([HA]/[H2A+]) with, [HA] + [H2A+] = 1 mM [HA] = 1…

Q: For lysine and arginine, the isoelectric point, pI, occurs at a pH where the net charge on the…

A: The isoelectric point for the amino acid Lysine can be calculated by using the pKa values of α-amino…

Q: The maximum number of amino acid side chains that can potentially form an ionic bond (+lil-…

A: Histidine is an amino acid with a imidazole ring attached as a substituent. The polarity of…

Q: Show the shift of the electron density and the appearance of partial charges on carbon atoms in the…

A: Given : To determine the electron density and the appearance of partial charges on carbon atoms in…

Q: The amino acid His is triprotic with pK1 (α-COOH) = 1.82, pK2 (α-NH3+) = 9.17, and pK3 (side chain)…

A: If the pKa of acid is equal or lower than the pH of the solution then the acid will get deprotonated…

Q: Consider this amino acid shown here at pH 7.0 (the isoelectric point for this amino acid is 9.7).…

Q: The amino acid histidine has ionizable groups with pKa values of 1.8, 6.0, and 9.2, COOH H₂N-CH CH…

A: GIVEN The pKa1, pKa2 and pKa3 values of Histidine are given as 1.8, 6.0 and 9.2. Initial pH of…

Q: Calculate the pH at which the e-amino group of lysine is 25% dissociated.

A: Step : 1 Calculate the pH at which the e-amino group of lysine is 25% dissociated.

Q: Sketch a titration curve for the following amino acids and indicate the pKa values for all…

A: Answer -

Q: What trend can you suggest for the stability of proteins under varying pH

A: As we decrease the pH (by adding acid ) converts COO- ion to a neutral COOH group. In each case the…

Q: Calculate the number of millimoles(mmol)in 500 mg of each of the following amino acids: alanine…

A: The relation between mass, molar mass and mole is given by : Mole = mass/molar mass

Q: Where is the effective buffering range for this amino acid in the acidic region?

A: Buffer is the solution that resists change in pH when small amount of acid or base is added to it.…

Q: (b) Describe how the charge of some amino groups in a protein might differ at pH 9.0 and pH 5.0.…

A: Polypeptides are polymers of amino acids that make up proteins. The amino (–NH2) and carboxylic acid…

Q: Draw a structural formula for the form of amino acid most prevalent at pH 1.0. (a) Threonine

A: Interpretation- To draw the formula of Threonine for the form of amino acid most prevalent at pH 1.0…

Q: Illustrate the lONlZATlON STRUCTURES and determine the isoelectric point (pI) of the given amino…

A: We have to illustrate the lONlZATlON STRUCTURES and determine the isoelectric point (pI) of the…

Q: Consider a histidine residue within a protein. Based on the histidine side-chain pKa value of 6.0…

A: Histidine is a basic amino acid used to make up proteins which contain an amino group and a…

Q: The fully protonated form of the amino-acid glycine (R=H) may be simply written as H2Gly+. Write the…

A: Glycine is the simplest stable amino acid, with the chemical formula NH₂‐CH₂‐COOH. Glycine is an…

Q: ine is a diprotic amino acid with a pk = 2.344 for the carboxylic group and a pK, = 9.868 for the…

Q: The pKa values of an amino acid are the following: pK1 = 2.18; pK2 = 8.95; pK3 = 10.53, what is…

A: Tha pKa values indicates ; pK1 = 2.18 ( this is the pKa of acidic group) pK2 and pK3 are the pKa…

Q: The above amino acid, shown at pH = 7.0 and where every atom (including %3D hydrogens!) is visible,…

A: Amino acids are molecules that combine to form proteins. Amino acids and proteins are the building…

Q: The maximum number of amino acid side chains that can potentially form an ionic bond (+lI-…

A: The structure of side group is shown below: It has two Nitrogen atoms in the ring capable of…

Q: The state of ionization of a specific amino acid is directly influenced by the pH of the surrounding…

A: The state of ionization depends upon the pH of the medium. Lysine is a basic amino acid having two…

Q: The amino acid sequence shown below is a short excerpt (residues 130-172) from the rod domain of an…

A: Following the rules of conservative amino acids, the amino acids that when replaced with a…

Q: What is the name of this oligopeptide? Use the three letter and one letter nomenclature of the amino…

A: Two amino acids are linked to each other through a peptide bond [C(=O)-NH-]. Hence "n" number of…

Q: If prior to the analysis, the protein X needs to be solubilised in 250 mM acetate buffer, pH 4.0 to…

Q: Given the chart of pKa’s below, calculate the isoelectric point for tyrosine

A: Isoelectric point (pI):The isoelectric point is the pH of an aqueous solution of an amino acid (or…

Q: An amino acid residue side chain is deprontonated at pH9.5. What is the pKa of this residue if it is…

A: an animo acid is a biomolecule which as both basic amine group and acidic group ( carboxylic acid)…

Q: A solution of the amino acid Lysine is at pH 13. What will be the overall charge, the charge on the…

Q: Which of the common amino acids is not chiral? Sketch its structure and explain why it does not…

A: Glycine is the amino acid which not chiral i.e, Achiral. The structure of Glycine H2N - CH2C ( = O…

Q: For aspartic and glutamic acids, the isoelectric point occurs at a pH where the net charge on the…

Q: Draw a structural formula for the form of amino acid most prevalent at pH 1.0. (a) Methionine

A: Amino acids are essential biomolecules . There are about 20 amino acids which includes both acids ,…

Q: A solution of amino acid having carboxylic side chain was titrated against NaOH. If initial pH of…

Q: (a) Describe in detail how you will determine the primary structure of protein. You have been given…

A: (a) Primary structure of protein The primary structure of a protein is generally referred to the…

Q: The pK1, pK2, and pKR for the amino acid cysteine are 1.9, 10.7, and 8.4, respectively. At pH 5.0,…

Q: The pKa for the side chain amino group in lysine is 10.4. If we were working at physiological pH…

A: At pH below the pKa for the amino acid is protonated and at pH above the pKa then it is…

Q: The secondary structure of a protein is the Multiple Choice Ospecific order of amino acids in the…

A: Secondary structure refers to regular, recurring arrangements in space of adjacent amino acid…

Q: Which amino acid has the greatest amount of negative charge at pH = 6.20?

A: The has two group over it the acid group and they carry both charges depending on the pH of both the…

Q: For the amino acid glutamate, what fraction of the side-chain (R-group) will be ionized at pH = 3.5?…

Q: The isoelectric point (pl) of phenylalanine is pH 5.5. Draw the structure of the major form of…

Concept explainers

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

Question

What is the charge of each amino acid residue in the peptide chain and what is the net charge on the whole protein at pH = 4 (Use the provided table)?
KGL
pka Value
Alpha Carboxy |+Alpha Amino Side Chain
Amino Acid
Name
Glycine
Alanine
Valine
2.34
9.60
2.34
9.69
2.32
9.62
Leucine
2.36
9.60
Non-Polar Amino Acids Isoleucine
Methionine
Phenylalanine
Tryptophan
2.36
9.68
2.28
9.21
1.83
9.13
2.38
9.39
Proline
1.99
10.60
Serine
Threonine
Cysteine
Tyrosine
Asparagine
Glutamine
2.21
9.15
2.63
9.10
1.71
10.78
8.33
Polar Amino Acids
2.2
9.11
10.07
2.02
8.84
2.17
9.13
Aspartic Acid
Glutamic Acid
Lysine
Arginine
2.09
9.82
3.86
Acidic Amino Acids
2.19
9.67
4.25
2.18
8.95
10.79
Basic Amino acids
2.17
9.04
12.48
Histidine
1.82
9.17
6.04

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

A 1.00-mg sample of a pure protein yielded on hydrolysis 0.0165 mg of leucine and 0.0248 mg of isoleucine. What is the minimum possible molar mass of the protein? (MMleucine=MMisoleucine=131g/mol)
Finally, you want to make completely certain that you purified the correct protein and not some other protein (like biochemisnotfunase). To confirm the identity of your protein, you run a Western blot. When doing a Western, one typically needs a solution of phosphate-buffered saline, which is comprised of 10 mM phosphate buffer(pH 8.0) and 0.9% (w/v) NaCl. Describe how you would make 500 mL of this solution including the grams of sodium phosphate, monobasic and sodium phosphate, dibasic that you would need. Making the phosphate buffer is a little tricky. Be sure to use the correct ratio of monobasic and dibasic sodium phosphate to get the correct pH. The Henderson- Hasselbalch equation might come in handy here. Finally, the molecular weight of sodium phosphate, monobasic (NaH2PO4) is 119.98 g/mol and the molecular weight of sodium phosphate, dibasic (Na2HPO4) is 141.96 g/mol.
The amino acid His is triprotic with pK1 (α-COOH) = 1.82, pK2 (α-NH3+) = 9.17, and pK3 (side chain) = 6.00. What is the net charge on His at pH 8?
A peptide has the sequence Glu–His–Trp–Ser–Gly–Leu–Arg–Pro–Gly The p?a values for the peptide’s side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pKa Carboxyl pKa Side‑chain pKa glutamate 9.609.60 2.342.34 4.254.25 histidine 9.179.17 1.821.82 6.006.00 tryptophan 9.399.39 2.382.38 serine 9.159.15 2.212.21 glycine 9.609.60 2.342.34 leucine 9.609.60 2.362.36 arginine 9.049.04 2.172.17 12.4812.48 proline 10.9610.96 1.991.99 Calculate the net charge of the molecule at pH 11 and estimate the isoelectric point (pI)(pI) for this peptide.
For the amino acid alanine, the major species in solution at pH 7 is the zwitterionic form, which has a negatively charged carboxylic acid group and a positively charged amino group. There is a less common neutral form in which neither group is charged. The carboxylic acid group of alanine has a p?apKa of 3.3. The amino group of alanine has a p?apKa of 8.8. Estimate the ratio of the concentration of the neutral amino acid species to the zwitterionic species at pH 7.
Q1)In the pH range 1.82 to 8.99, H2Arg+ is the principal form of arginine. Which is the second most prominent species at pH 6.0? At pH 5.0?Q2) (a) Draw the structure of the predominant form (principal species) of 1,3-dihydroxybenzene at pH 9.00 and at pH 11.00.(b) What is the second most prominent species at each pH?(c) Calculate the percentage in the major form at each pH
The pKa values of an amino acid are the following: pK1 = 2.18; pK2 = 8.95; pK3 = 10.53, what is the isoelectric point of the amino acid? Show the solution and express the your answer in three significant figures.
Almost all proteins are composed from a set of about _____ amino acids.a. 4b. 10c. 20d. 50 ______ amino acids are considered essential.a. 5b. 10c. 15d. 20 An amino acid that has an indole group side chain.a. Trpb. Phec. Tyrd. ProWhich of the following is the simplest amino acid?a. Alab. Glyc. Vald. ProWhich of these will not give a blue solution with Ninhydrin testa. Prob. Phec. Alad. CysA neutral molecule with a positive and negative electrical charge.a. Isoelectric pointb. Zwitterionsc. Bufferd. Amphoteric3 amino acids joined together by two peptide bonds.a. Dipeptideb. Tripeptidec. Tetrapeptided. PolypeptideAn amino acid that can form disulfide bondsa. Cysteineb. Methioninec. Cystined. SerineWhich of the following is not a property of amino acidsa. Bufferb. Isoelectric pointc. Zwitterionsd. Insoluble in waterThe protein primary structure is held together bya. Hydrogen bondsb. Disulfide bondsc. Peptide bondsd. Protein bonds
The denaturing of proteins occurs when the stabilizing forces are altered. Below is the set of processes in which proteins are denatured. Classify the following processes according to the denaturing agent involved. Please help me understand the diff and correct any mistakes.
A typical amino acid with one amino group and one carboxylicacid group, such as serine, can exist in water in several ionic forms. (a) Suggest the forms of the amino acidat low pH and at high pH. (b) Amino acids generally havetwo pKa values, one in the range of 2 to 3 and the other inthe range of 9 to 10. Serine, for example, has pKa values of2.19 and 9.21. Using species such as acetic acid and ammoniaas models, suggest the origin of the two pKa values.(c) Glutamic acid is an amino acid that has threepKa's: 2.10, 4.07, and 9.47. Draw the structure of glutamicacid, and assign each pKa to the appropriate part of themolecule. (d) An unknown amino acid is titrated withstrong base, producing the following titration curve.Which amino acids are likely candidates for the unknown?
A student added a total of 75 mL sodium hydroxide to titrate an amino acid solution with 0.5M NaOH. They also calculated that they added a total of 14 mL of sodium hydroxide to arrive at pH 2.6 and a total of 32 mL of sodium hydroxide to arrvive at pH 4.8.What was the initial concentration of the amino acid solution in milimolar (mM)? The calculations must be presented such that those can be recapitulated by the marker. Provide the answer with no decimal places. Show all details of your working out. State the answer in a full sentence.
A peptide has the sequence Glu–His–Trp–Ser–Gly–Leu–Arg–Pro–GlyGlu–His–Trp–Ser–Gly–Leu–Arg–Pro–Gly The p?a values for the peptide’s side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pKa Carboxyl pKa Side‑chain pKa glutamate 9.609.60 2.342.34 4.254.25 histidine 9.179.17 1.821.82 6.006.00 tryptophan 9.399.39 2.382.38 serine 9.159.15 2.212.21 glycine 9.609.60 2.342.34 leucine 9.609.60 2.362.36 arginine 9.049.04 2.172.17 12.4812.48 proline 10.9610.96 1.991.99 Calculate the net charge of the molecule at pH 8