(a) Describe in detail how you will determine the primary structure of protein. You have been given a mixture of lysine, histidine and cysteine. The isoelectric point of the amino acids are as follows: Histidine 7.64 Lysine 9.74 Cystenie 5.02 Show how you will separate the mixture into the pure forms. State and describe any instrument that you will use to separate the components in the mixture.

Organic Chemistry
8th Edition
ISBN:9781305580350
Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Chapter27: Amino Acids And Proteins
Section: Chapter Questions
Problem 27.45P
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(a) Describe in detail how you will determine the primary structure of protein.

You have been given a mixture of lysine, histidine and cysteine. The isoelectric point of the amino acids are as follows:

Histidine 7.64

Lysine 9.74

Cystenie 5.02

Show how you will separate the mixture into the pure forms. State and describe any instrument that you will use to separate the components in the mixture.

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