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- The most common test used for the measurement of hormones is: A) immunoassay B) colorimetric spectrophotometry C) high performance liquid chromatography D) ion selective electrodeVitamin C Analysis, a student accidentally puts 1.0 g of sodium bicarbonate (NaHCO3) solid into the vitamin sample solution for trial #1 instead of 0.5 g as the procedure instructs. How does this procedural change impact the calculated milligrams of ascorbic acid in the sample?What is the application of chromatography (HPLC) and it's mechanism ?
- Determination of ascorbic acid in vitamin C tablets: which analytical approach is better; volumetric or spectrophotometric analysis?(Please answer all questions) 5-33. What are some advantages of using column chromatography to purify reaction products in the microscalelaboratory? 5-34. Discuss the similarities and dissimilarities of TLC, paper, and column chromatography. 5-35. Discuss the similarities and dissimilarities of HPLC and gas chromatography. 5-36. (a) What are the main advantages of using flash chromatography?(b) How can TLC be used in connection with flash chromatography? 5-37. Using the information presented on the right, please identify and explainwhich spot has an Rf value of 0.5.An HPLC method was developed for the separation and determination of ibuprofen in blood samples. A 3.00 mL blood sample dissolved in 70.00 mL solvent was prepared and 10.0 mL was injected into the HPLC. Standard ibuprofen solutions were also run.Calculate the ibuprofen present in the 3.00 mL blood sample The following data were obtained. Table po itoo: Ibuprofen concentration, mg/ml0.50 1.0 2.0 3.0 Blood sample Peak area (Right column)5.29.617.419.911.4
- The following data represented the concentration of ofloxacin present in chicken meat samples: 4.17, 4.40, 4.35, 4.35, 4.20, 4.16,4.20 and 4.17. Estimated the % relative standard deviation.qualitative and quantitative aspects of HPLC?3 to the kit manual 200uL of plasma was mixed with 2.8 ml of the dilution buffer, mixed well then 200 uL of the dilution mixture was pipetted in 0.9 ml of solution A and 0.9 mL of solution B and measured using a spectrophotometer, the concentration was 30. What is the final concentration of the sample after adjusting the dilution factor
- A 10 ml of ascorbic acid (MW=176.1) injection was transferred to a 100 ml volumetric flask and made up to volume with deionized water. A 20 ml of the diluted injection was transferred to a conical flask and titrated with Iodine VS using starch mucilage as the indicator. The iodine solution was standardized against a solution of sodium thiosulfate primary standard (MW=248.2). Results: Weight of sodium thiosulfate used to prepare 100 mL primary standard solution = 2.501 g Volume of thiosulfate solution required to titrate 25 mL of Iodine solution = 24.83 mL Volume of iodine solution required to titrate 20 mL of the diluted ascorbic acid injection = 21.55 mL Stated content of the injection = 10% w/v Calculate the % of the stated content in the injection. (Round-off your answer to 2-decimal places) Answer = ____%A 10 ml of ascorbic acid (MW=176.1) injection was transferred to a 100 ml volumetric flask and made up to volume with deionized water. A 20 ml of the diluted injection was transferred to a conical flask and titrated with Iodine VS using starch mucilage as the indicator. The iodine solution was standardized against a solution of sodium thiosulfate primary standard (MW=248.2). Results: Weight of sodium thiosulfate used to prepare 100 mL primary standard solution = 2.501 g Volume of thiosulfate solution required to titrate 25 mL of Iodine solution = 24.83 mL Volume of iodine solution required to titrate 20 mL of the diluted ascorbic acid injection = 21.55 mL Stated content of the injection = 10% w/v Calculate the % of the stated content in the injection.106.54 mg of an impure sample of the drug was diluted in 100 ml and injected in a HPLC, the AUC of the major peak in the chromatogram integrated 1140 units. A standard solution containing 1.13 mg/ml of this drug was then injected in the same HPLC and the resulting peak integrated 1912 units. What is the %w/w of purity for this sample?(One decimal place, no units)