what is the main purpose of performing the bioinformatics analysis of 16s rRNA genes lab?

Q: What is an advantage of making a cDNA library rather than a genomic library?

A: DNA library or the gene library is the collection of DNA sequences from different organisms. These…

Q: Discuss how CRISPR-cas functions as a genome editing tool.

A: Genome editing:It is also known as gene editing or genome engineering. It is a type of genetic…

Q: What are the differences between a DNA library and acDNA library?

A: For the gene cloning method, the DNA is used, which is cut and ligated in the vector. This…

Q: ACAACCCCAAGCCTTC

A: Answer : PROBE - DNA probes are the short stretches of single stranded DNA used to detect the…

Q: What is the minimum and maximum cDNA input from FFPE samples in library preparation phase to perform…

A: Next genome sequencing is quickly turning into the technique for decision for transcriptional…

Q: List the critical steps in the ribosome profiling protocol and explain what this method is useful…

A: Ribosome profiling- it is carried out on a split sample, with parallel libraries constructed for…

Q: Question:- Can you please explain the general rule on how to manually align these sequence?? i am…

A: DNA sequencing is a technique for determining the exact sequence of bases (A, C, G, and T) in a DNA…

Q: Describe the methods of site-directed mutagenesis and CRISPR-Cas technology.

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: Why do geneticists studying eukaryotic organisms often construct cDNA libraries, whereas…

A: A cDNA (complementary deoxyribonucleic acid) library is a combination of cloned cDNA fragments…

Q: How is ribotyping similar to rRNA sequence analysis? How do the two techniques differ? Do you think…

A: RFLP is a method to check genetic markers in the genome of organisms. The genetic markers are…

Q: principles,  advantages disavantages of DNA and RNA microarrays  and Taqman SNP analysis and their…

A: SNP stands for single nucleotide polymorphisms. It is a polymorphisms in which variation in DNA…

Q: How are shotgun sequencing, bioinformatics, and proteomics related to genome projects?

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: Briefly outline the components of the CRISPR/Cas system

A: Nonspecific endonuclease (Cas9 or Cpf1 closely associated) is used by CRISPR / Cas9 genome editing…

Q: What are the possible bioethical issues that gene editing tools may encounter?

A: Gene editing tools are used to edit an organisms genetic code , i.e.. with these tools ,the genetic…

Q: What is Central Dogma of Molecular Biology?

A: The organisms that contain DNA as a genetic material shows a fascinating process within the cell is…

Q: Why the sequence alignment is extremely important in designing a construct for developing a…

A: Sequence alignment It is defined as the way of arranging the sequences of DNA, RNA or protein to…

Q: What is a cDNA library, and for what purpose can it be used?

A: In genetics, the term cDNA library refers to the library containing the group of cloned copies of…

Q: To clone a bacterial lipase gene into the cloning vector pET28a, you know the full sequence of the…

A: For the ease of manipulation, vectors must be relatively small molecules. They must be capable of…

Q: What are the vectors used in rDNA technology? briefly explain each

A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…

Q: What are the two advantages of using sequence analysis of ribosomal components in determining the…

A: Ribosome is an essential component of cellular machinery that is present across all life forms. The…

Q: How are cDNA libraries made?

A: There are two types of human libraries. These are genomic library and cDNA library. The human…

Q: Who developed the sequencing-bysynthesis (SBS) approach? & When it was firstly used ?

A: Sequencing by synthesis (SBS), an Illumina sequencing method, is a commonly used next-generation…

Q: What has been the most significant finding of the ENCODE project?Why was the project expanded to…

A: ENCODE stands for Encyclopedia of DNA elements. It was a research project that came after the Human…

Q: What is genetic engineering? What organisms can it be performed on? Please give an example of at…

A: * Genetic engineering is also known as gene manipulation or modification. * It is a process to alter…

Q: The following DNA sequences were used to generate a contig from a genome sequencing project.…

A: Sequencing It is a method through which the complete genetic makeup of the organism is identified.…

Q: What is the motive behind Genome Project 10K?

A: The advent of low-cost, high throughput sequencing has ushered in a new age of genome science and…

Q: Why is GenBank important and What is GenBank format?

A: GenBank is the Genetic sequence database at NCBI (National center for biotechnology information). It…

Q: What is the purpose of Southern's blotting technique? Explain in detail the biochemical principle…

A: The purpose of Southern blotting technique is  to identify specific DNA from the mixture of other…

Q: Where did the Crispr system first originate and what is its purpose in nature? What is the advantage…

A: We are answering first 4 questions. For rest of questions pls repost.

Q: For the method of RNA sequencing (RNA-Seq), which of the followingis the correct order of steps?a.…

A: RNA sequencing is a method that is used to identify expressed genes. A transcriptome is defined as…

Q: Please answer ASAP RNA sequencing (RNA-Seq) of one sample in one run of a massively parallel…

A: Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers…

Q: What is an advantage of RNA Seq over microarrays?

A: The process in which the sequencing of total messenger ribonucleic acid (mRNA) is carried out…

Q: Can Bioinformatics be used for sequence annotation to identifyprotein-coding and noncoding sequences…

A: The genome sequence of any organism is that organism's blueprint: the set of instructions dictating…

Q: What is the purpose of the dideoxyribonucleoside triphosphates in the dideoxy-sequencing reaction?

A: DNA sequencing is carried out using various methods. The majorly used method of DNA sequencing is…

Q: Consider the following human genetic diseases: hemophilia, Down syndrome, cystic fibrosis, and brain…

A: CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats) Cas genome editing technology…

Q: What is the difference between bulk RNAseq and single cell RNA seq?

A: In RNA sequencing,  RNA is taken out from tissues of different cell types for analysis of RNA seq .…

Q: Discuss the importance of adding radioactively labeled molecules during 16S ribosomal RNA sequencing…

A: The bacterial isolates can be identified accurately by the 16S ribosomal ribonucleic acid (rRNA). It…

Q: I recently isolated the human enzyme called fucosidase and prepared anantibody to it. Now I want to…

A: A probe is one of the essential components necessary to identify a gene during cloning. A probe is…

Q: Why is 16s rRNA used as a target in bacterial species identification? Would 23s rRNA be a better…

A: The small subunit ribosomal RNA molecules of ribosomes are encoded by the 16S rRNA gene. It is…

Q: What are the similarities and/or differences between interpreting Multiple Sequence Alignment (MSA)…

A: Multiple sequence alignment (MSA) is the process or outcome of aligning three or more biological…

Q: TALENs What is it? And how it works in genome editing? Including

A:

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.