What is the purpose of plasmids found naturally in bacteria? Plasmids contain the majority of the genes found in a bacterial genome Plasmids are essential for meiosis in bacteria Plasmids contain restriction enzymes that allow them to cut the DNA in human cells Plasmids often contain genes that allow bacteria to survive treatment with certain chemicals

What is the purpose of plasmids found naturally in bacteria? Plasmids contain the majority of the genes found in a bacterial genome Plasmids are essential for meiosis in bacteria Plasmids contain restriction enzymes that allow them to cut the DNA in human cells Plasmids often contain genes that allow bacteria to survive treatment with certain chemicals

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 4RQ: What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel...

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Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.
What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reaction
DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c) ionic bonds (d) disulfide bonds (e) phosphodiester linkages
The following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloning
Small circular molecules of DNA in bacteria ____. a. result from the activity of restriction enzymes b. are plasmids c. cannot survive outside of the cell d. are eventually degraded e. are DNA fragments from their main chromosome
A plasmid differs from the bacterial chromosome in that it is __________. A. significantly smaller B. always found as a single copy of DNA in a cell C. circular D. double-stranded A. significantly smaller B. always found as a single copy of DNA in a cell C. circular D. double-stranded
When referring to bacteria cells, a plasmid is not naturally occuring. is the large bacterial chromosome contained in the nucleoid. is a small, circular double-stranded DNA molecule that replicates autonomously. is composed of double-stranded RNA. does not code for proteins.
How is bacterial DNA safe from digestion by Restriction Endonucleases? The plasmid of the bacteria provide antibiotic resistance Bacteriophage provide immunity to DNA degradation Foreign DNA outcompetes the chromosomal DNA Modification by methylation blocks the restriction enzyme action
Which of the following would be a good application of plasmid transformation? 1) to make copies of DNA 2) to isolate a change in a single nucleotide 3) to separate DNA fragments 4) to sequence DNA
Plasmids differ from transposons because plasmids become inserted into chromosomes self-replicate outside the chromosome move from chromosome to chromosome carry genes for antibiotic resistance none of the above
Which statement is NOT the function of restriction enzyme? Group of answer choices It cuts the vector’s plasmid and the desired gene from the original DNA. It ligates the desired to gene to vector’s plasmid. It acts as scissors of DNA that cut along a specific sequence. It serves as degrading proteins that cut plasmid on specific target site.
A.) Transformation is best described as: Group of answer choices The integration of foreign DNA into a genome The uptake of naked DNA from the environment The transfer of DNA via a bacteriophage Transfer of a plasmid from one organism to another B.) What is the function of the araC gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells C.) What is the function of the bla gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on…