Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the process of extracting the DNA.
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- Why do we need to add “COLD” alcohol? What is the effect of the temperature of the alcohol in the process of extracting the DNA.
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- Why is it important that the alcohol used in the DNA extraction is kept cold?The last steps of DNA extraction include washing it using ethyl alcohol and drying the DNA pellet. Why do we need to wash the DNA using ice-cold ethyl alcohol? And why do we have to dry the DNA pellet?Why do alcohol and water separate in DNA extraction process?
- What special kind of nucleotide is used in the Sanger sequencing procedure?What does the alcohol do in this experiment? Why does the DNA rise to the top after adding alcohol? For reference, this is the procedure for the experimentWhether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather than agarose. Why can't agarose be used for a sequencing gel, as it is for other DNA gel electrophoresis?