36. Which is the sequence of steps used to amplify DNA with PCR during a single cycle? 1) Primers bind (anneal) to complementary sequences of the target DNA. 2) DNA is heated to a high temperature to cause denaturation (DNA is single-stranded). 3) DNA polymerase extends the 3’OH of primers to make copies of the target DNA. A) 1, 2 and 3 B) 3, 2, and 1 C) 2, 1, and 3 D) 1, 3, and 2

36. Which is the sequence of steps used to amplify DNA with PCR during a single cycle? 1) Primers bind (anneal) to complementary sequences of the target DNA. 2) DNA is heated to a high temperature to cause denaturation (DNA is single-stranded). 3) DNA polymerase extends the 3’OH of primers to make copies of the target DNA. A) 1, 2 and 3 B) 3, 2, and 1 C) 2, 1, and 3 D) 1, 3, and 2

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 12TYK: Discuss Concepts A forensic scientist obtained a small DNA sample from a crime scene. In order to...

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We usually think of enzymes as being most active at around 37°C, yet in PCR the DNA polymerase is subjected to multiple exposures of relatively high temperatures and seems to function appropriately at 65–75°C. What is special about the DNA polymerase typically used in PCR?
The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.
Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA ligase _____ cDNA synthesis c. reverse transcriptase _____ DNA sequencing d. restriction enzyme _____ pasting DNA e. DNA polymerase (not Taq)
When Griffith injected mice with a combination of live rough-strain and heat-killed smooth-strain pneumococci, he discovered that (a) the mice were unharmed (b) the dead mice contained living rough-strain bacteria (c) the dead mice contained living smooth-strain bacteria (d) DNA had been transferred from the smooth-strain bacteria to the mice (e) DNA had been transferred from the rough-strain bacteria to the smooth-strain bacteria
Discuss Concepts A forensic scientist obtained a small DNA sample from a crime scene. In order to examine the sample, he increased its quantity by cycling the sample through the polymerase chain reaction. He estimated that there were 50,000 copies of the DNA in his original sample. Derive a simple formula and calculate the number of copies he will have after15 cycles of the PCR.
What is the purpose and benefit of the polymerase chain reaction?
Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro?
35) A researcher inserted DNA fragments from an organism into expression vector plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be an effective means of identifying which clones contain a specific gene of interest?
What is the role of alcohol in extracting DNA? 1.DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. 2.DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. 3.DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. 4.DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.
DNA Polymerase holoenzymes used for DNA replication recognizes A. double-stranded sequences as starting points B. methylated lipids as start points C. acetylated lipids as start points D. single stranded sequences as starting points
1. What is the function of the DNA polymerase enzyme in the PCR? 2. What natural process is PCR based on?
Why is the use of temperature-stable DNA polymerase animportant factor in the polymerase chain reaction?