What is the purpose of a standard curve for a colorimetric enzyme assay?
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What is the purpose of a standard curve for a colorimetric enzyme assay?
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- Describe the p-nitrophenol assay and how a standard curve can be used to calculate the concentration of an unknown .Why do we use standard solutions in protein assays? Explain briefly.Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?
- For the following sequence, what is the approximate annealing temperature? 5'-AGCTACGATCAGGTCA-3'What is transformation efficiency and how is it calculated?What reaction would you expect when performing a positive control in the catalase assay? What would it mean if a known catalase-positive bacteria did not produce the expected reaction?
- There are three names that often get used when discussing the Lowry assay. Those names are: Lowry, Folin-Ciocalteu, and Biuret. Are those names interchangeable when discussing the assay or do those names describe the same thing(s)? Significant differences? What is the relationship between those names?Why is it essential to add sodium azide to succinate dehydrogenase assay solution? What is the purpose of including the sodium succinate solution?what are the advantages and disadvantages of using recombinant protein and affinity chromatography for protein purification compared to gel filtration (size exclusion chromatography) and DEAE-sepharose chromatography (ion-exchange chromatography)?
- How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein purification techniques?is bradford assay using dye Coomassie Brilliant Blue G-250 a different method from using a spectrophotometer ? if so how is it different to determine protein concentration?why are lysozyme and ovoalbumin the recommended standards used in the Bradford protein assay and why would they be preferred over BSA being this widely used but not recommended? Justify the answer
