What measures could you propose on the blade and on the microtome setting so that sections produced would be successful?

Q: Briefly discuss the following terms: Coarse facing Coarse trimming Fine trimming Cold wax Floatation…

A: A microtome is a device employed in cutting sections from an embedded specimen to be used as a…

Q: What is the stain responsible for staining the vegetative part of this stained specimen?

A: Microbiology is the study of microorganism. The organisms that cannot be seen through naked eyes are…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: If the sharpness of V-notch is more in one specimen than the other, what will be its effect on the…

A: If the sharpness of V-notch is more in one specimen than the other then the one which is having…

Q: What situations would result in recollection or rejection of specimen? Determine  corresponding…

A: Specimen: a single animal, plant, mineral fragment, or other object used for scientific study or…

Q: In what instances would the pour plate method be more appropriate than the spread plat method?

A: Pour plate method is a technique that used to enumerate the number of viable cells in a sample.…

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…

Q: eservatives cannot be used for direct + mount ?

A: The disease is a state of the body in which the physiological mechanisms do not function in a normal…

Q: aseptic technique and pure culture technique

A: A‌SEPTIC TECHNIQUE_ *Inserting PICC lines. *Performing dialysis.‌ *Inserting catheters.‌ *Running…

Q: What are the purposes of "BAG TECHNIQUE PROCEDURE" and why is it important that you should properly…

A: Community bag is a type of tool that is used for home visit and enable community health nurse to…

Q: What is the difference between the pour plate method and the spread plate method? Why might you…

A: Standard plate count method is a growth based approach that counts the number of living…

Q: Why is there a need to use a staining agent in observing the specimen?

A: Staining agent:          The things that owes-out stains were recognized as staining agent,…

Q: Identify one culture-based and one non-culture-based method used for analyzing bacterial growth.

A: Bacteria divide by binary fission. They grow in an exponential manner. Four different phases can be…

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: What result do you report for the slant portion of your TSI agar slant?

A: The Triple Sugar Iron (TSI) test This test is used to test the  microorganism's ability to ferment…

Q: Why is it important to use a sterilized loop between streaks when preparinga streak­plate?

A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…

Q: Which of the following alter name for method of false position? a.Method of chords  b.Regula-falsi…

A: Answer 1- b. Regula-falsi method.  regula falsi method or false position is the method for solving…

Q: Use the counting method to estimate the length of an elodea cell. You will need to use your…

A: If the magnification is 400x then the diameter of one single cell would be .04mm  To find the length…

Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…

A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…

Q: What advantage(s) does the pour plate method have over the streak plate method?

A: In microbiology. streaking is a technique for the isolation of the pure strain from the population.…

Q: When conducting the pGLO lab none of my plates developed growth. Not even the plates in which we…

A: The pGLO plasmid is used to produce the genetically engineered organism because it acts as a vector…

Q: What is the difference between streak plate method and spread plate technique?

A: Answer of the question given below...

Q: What do to do when the disstillation did not make the sample completely pure? What could have went…

A: Distillation is a process that is based upon the boiling points of various liquids present in the…

Q: What are the ways you can control the amount of light passing through your specimen? One way is…

A: A specimen is a sample which represents the whole group. The specimen can anything which is a…

Q: How is agar in the petri dish prepared to grow the bacterial specimen? explain in your own words.

A: Agar is a polymer made up of subunits of sugar galactose and is a component of cell walls of several…

Q: what are the advantage and disadvantage using cotton plug in laboratory

A: Adavantages :  Free Caps, which include cotton plugs , allow air to flow free;y on the perimeters of…

Q: Draw and explain the process of electrophoresis utilized in decalcification.

A: Decalcification :-  As name suggests, it is removal of calcium salts,minerals and calcium tissues…

Q: If you observe a specimen under this 20X objective and the specimen spans one-quarter of the field…

A: A light microscope is common equipment in most laboratories. It comes with one or two eyepieces…

Q: What result do you report for the butt portion of your TSI agar slant?

A: The Triple Sugar Iron (TSI) test helps to test the ability of microorganisms to ferment sugar and to…

Q: Activity: Amoeba Procedure: Label the following structures on the picture (only the numbers that are…

A: Amoeba is a unicellular organisms which is capable of altering it's shape mainly through extension…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-° dilution to a sterile petri…

A: Dilution is a process by which solution (water or diluents) are added to the solvent (main solution)…

Q: When labeling culture tubes or plates why do we use a pencil instead of a pen?  Are there other…

A: Appropriately labeling specimens for research labs is of critical importance.

Q: Wintrobe from Westergreen regarding accuracy of the test in a tabulated order.

A: Both methods are used for estimating E.S.R rate or the erythrocyte sedimentation rate. Most of the…

Q: -1 mm dentify the given specimen ? is it from a vasular plant or non-vascular plant? For the…

A: The given specimen appears to be green in color suggestive of capability to perform photosynthesis.…

Q: Name the substance used as a medium in gel electrophoresis.

A: Electrophoresis is the process of separation of charged macromolecules under the influence of…

Q: If you put a fresh milk sample in a test tube, and put it in centrifuge and started centrifuga on…

A: Centrifugation is a technique which is used to separate mixtures by applying centrifugal force on…

Q: If accessories for darkfield microscopy were not available, how would you construct a simple star…

A: The microscope is an instrument used to view small objects that cannot be seen with an unaided eye.…

Q: What are the key points necessary for proper collection ofclinical specimens, and why is it…

A: Clinical specimens are those biological specimens taken from a patient's body that will be used for…

Q: 1. What is the significance of use of potato in the preparation of culture media? 2. What is the…

A: To identify the microorganism, it must first be placed into a medium to commence development, and…

Q: Please provide the Preferred Specimen/Tissue Medium with brief explanation of the following types of…

A: Microtome: it is a cutting tool which is used to generate extremely thin slices of material which is…

Q: Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big…

A: Petri plates, slants and stabs of Culture media such as Luria Bertani agar is used in microbiology…

Q: What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail…

A: Errors and limitations of gel electrophoresis.

Q: Discuss the ways of measuring the extent of decalcification, post decalcification procedures, and…

A: 1. Mechanical test- done by touching or bending the fingers to determine tissue consistency.  2. X…

Q: If the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X…

A: Given, Field diameter - 2.4 mm Cells across the field of view - 16 cells Objective Lens - 40X The…

Q: You are asked to calibrate the ocular micrometer for the 4x objective using the stage micrometer.…

A: Length of stage micrometer  is 1mm  with 100 divisions ,so each division is of size one-hundredth of…

Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…

A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.

Q: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test…

A: Introduction Lysogeny Broth (LB) Is A Nutrient-dense Media Predominantly Used For Bacterial Growth.…

Q: Write the procedure for resin embedding

A: Answer: EMBEDDING = It is the process of embed a tissue for fine and regular sectioned, the most…

Q: What is the purpose of examining your specimen slide using low power objective first nefore high…

A: Objective lens are magnifying lens that functions to magnify an image. These lens make an important…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.