What would your final dilution be if you transferred 0.1 mL of a 10-° dilution to a sterile petri dish and then added molten agar to make a pour plate?
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- How would you prepare a 10-7dilution from a culture? Each of your dilution tubes cannot hold more than 1mL.If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar plate, what is the final dilution?To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.
- If you diluted 200 uL of your culture into 1800 uL of water what is your dilution factor? (Show your work)You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor?A culture medium is comprised of the following components: 0.5% peptone, 0.3% meat extract, 1.5% agar, and distilled water. If you are asked to prepare 765 mL of culture media, how much meat extract would you need?
- If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramWhat is the EFFECT and RATIONAL of Molten agar mixed with bacterial suspension has temperature higher than 45 oC.How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
- You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show solutionYou have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volumeWhat is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 × 106 cells/mL?