When we perform Bradford Assay to measure protein concentration, the spectrophotometer is set  595 nm because:    a.  the excitation peak of Coomassie Blue is best observed at 595 nm   b.  when Coomassie Blue binds to proteins, its maximum absorbance shifts to 595 nm   c.  the reducing effects of Coomassie Blue shifts the protein's maximum absorbance to 595 nm   d.  myoglobin is best observed at 595 nm

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