Q: What is the usual stain used for staining a sputum smear?
A: Essentially, sputum comprises of a combination of saliva and mucus. additionally named as phlegm, It…
Q: Why is a block of agar inoculated rather than streaking the fungal culture on a plate?
A: The culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth…
Q: Why do you have to boil the agar solution before dispensing it into tubes?
A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…
Q: How to prepare smear and squash.
A: In general : Squash spreads were set up with little pieces of tissue estimating 1–2 mm size without…
Q: What is the principle application of negative staining?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: In what instances would the pour plate method be more appropriate than the spread plat method?
A: Pour plate method is a technique that used to enumerate the number of viable cells in a sample.…
Q: Give two reasons for heating the slide after the smear is air dried?
A: Smear is the thin film of the sample that is spread over the slide to be observed in the microscope.…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear. What are…
A: Many laboratory techniques, including the Gram-stain, involve the creation of a smear. The goal of…
Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…
A: Introduction Capsule is the outer most covering, which is present even outside the cell wall. This…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: What is the difference between the pour plate method and the spread plate method? Why might you…
A: Standard plate count method is a growth based approach that counts the number of living…
Q: When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times…
A: The initial identification of the streptococcal and enterococcal species is based on their hemolytic…
Q: What will the appearance of Escherichia coli be on MacConkey Agar red and Eosin Methylene Blue (EMB)…
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Q: Write a note on Hard gelatin capsules? Make the note on full making process etc
A: Introduction Hard-gelatin capsules are two cylindrical sections made of gelatin, known as the body,…
Q: uestions What is the purpose of the soft agar? 1
A: The questions ask about the role of soft agar.
Q: describe the agar-overlay technique
A: Biological techniques are processes or methods for trying to study living things. They include…
Q: How do we prepare smear
A: The preparation of a smear is required for many laboratory procedures, including the Gram-stain, for…
Q: What is the significance of heat fixing the smear?
A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…
Q: What is the difference between streak plate method and spread plate technique?
A: Answer of the question given below...
Q: How would you distinguish between Staphylococcus aureus and S. epidermidis on mannitol salt agar and…
A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which one…
Q: Area of no growth around an antibiotic disk indicates_________________.
A: Antibiotics are the substances that are produced synthetically or semi-synthetically from…
Q: Is it important to have a standardized way of counting and reporting AFB seen on a direct sputum…
A: Acid-Fast Bacilli (AFB) is a bacterial species that causes tuberculosis in humans and testing of…
Q: Why is it necessary to perform a negative stain to visualize capsules?
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Q: Would you consider all microorganisms growing from the planted tissue on PDA as the cause of the…
A: Potato Dextrose Agar (PDA) is a fungal cultivation medium. Potato Dextrose Agar (PDA) is a yeast and…
Q: What spectroscopic technique will identify fake and real honey? And how important it is to…
A: Honey is a naturally occurring sweetener that is originally derived from flower nectar and fruits.…
Q: When labeling culture tubes or plates why do we use a pencil instead of a pen? Are there other…
A: Appropriately labeling specimens for research labs is of critical importance.
Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?
A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…
Q: not more than five lines, outline a method for preparation of tomato cell suspension culture and…
A: 1. Callus: Plant callus in simple terms is a growing mass of unorganized parenchyma plant cells…
Q: What was the purpose of leaving the cover on one set of agar plates? How did the growth on these…
A: The only microorganisms that can grow anywhere are bacteria. They can grow on specially prepared…
Q: How should agar plates be incubated? Why?
A: Incubating the plates to stimulate the growth of microbes is a crucial step in any microbiology…
Q: Pretend you want to test whether a certain bacterium can take iron away from hemoglobin. What is the…
A: Culture media Culture media is a type of media that support the growth of bacteria and fungi and…
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: Write a detailed description of the following plate 1- blood Agar plate 2-PEa Agar Plate
A: An agar plate refers to a Petri dish containing an agar solidified growth medium which is meant for…
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: how could you determine if there is any contamination on your agar?
A: Agar consists of two polysaccharides, agarose and agaropectin, but agarose making up about 70% of…
Q: Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the…
A: Microorganisms are present in abundance in nature. When there are no aseptic conditions then…
Q: what is Rose Bengel Agar?
A: Rose Bengal Agar - Rose Bengal Agar is selective medium it is used to detect and enumerates yeast…
Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…
A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…
Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…
A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.
Q: During the preparation of a smear leading into simple staining of the bacterial culture S.…
A: S. epidermidis is a gram-positive bacteria.
Q: Why should freshly collected samples be used for microscopic examination?
A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…
Q: What is "BHI" agar and what is it used for? What is "Sabouraud Dextrose" agar and what is it used…
A: There are various types of growth media are used for cultivating cells ranging from prokaryotic to…
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: Write the procedure for resin embedding
A: Answer: EMBEDDING = It is the process of embed a tissue for fine and regular sectioned, the most…
Q: why is it important that you disinfect your work area both before and after working with bacteria?
A: Bacteria are present everywhere in each and every corner,present on non-living surfaces and as well…
Q: Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies…
A: Microorganisms are small organism that cannot be seen by naked eyes. Microorganism can be harmful to…
Q: What is the purpose of using the streak plate method?
A: Solution- Introduction - Streak plate technique is used for the isolation into a pure culture of…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
Where should a label be written on an agar plate?
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