Q: what might be the consequences of storaging coconut water at room temperature after collection and…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: Why is a block of agar inoculated rather than streaking the fungal culture on a plate?
A: The culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth…
Q: Could you spread plate in the usual Petri dish a 10mL inoculum? Why or why not?
A: The spread plate technique involves spreading a small amount of bacteria suspended in a solution…
Q: In spread plate method, colonies form within the agar and agar surface. In pour plate method,…
A: Introduction Agar media is essential for the study of microorganisms and molecular biology, It is a…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?
A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…
Q: You will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium,…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Agar is a solidifying agent used in media preparation. What is its origin?
A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…
Q: How does bacteria grow on agar plates?
A: Bacteria are the most primitive organisms on Earth. They can survive in most extreme environments as…
Q: What is the correct arrangement of the unknown bacterium growing on your MacConkey agar?
A: MacConkey agar (MAC) was the first solid differential media to be designed by Alfred Theodore…
Q: Where should a label be written on an agar plate?
A: The aseptic technique is applied in a laboratory setup to reduce the risk of contamination. It…
Q: You perform a Gram stain. Your Gram positive control, your Gram negative control, and your sample…
A: Gram-staining is a technique used to classify bacterial species, either as gram-positive or…
Q: Why is the time of exposure and temperature required for sterilization greater for dry heat than for…
A: Any technique that removes, destroys, or deactivates all forms of life and other biological agents…
Q: What bacteria grow on both a MacConkey agar plate and Mannitol Salt agar?
A: Selective media The media is a kind of growth media which only allow the growth of a perticular…
Q: What type of agar would you use to grow gram positive bacteria
A: Bacteria are different from the plant as well as animals. They require specific substances in the…
Q: Is it important to have a standardized way of counting and reporting AFB seen on a direct sputum…
A: Acid-Fast Bacilli (AFB) is a bacterial species that causes tuberculosis in humans and testing of…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not? (Answer in not more than…
A: Sterilization is defined as a process by which an agent is used to destroy or remove the…
Q: why macconkey agar and mannitol salt are regard as salective and deffrental medium?
A: A culture medium is just water and nutrients that support microbic growth. Primary culture media for…
Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…
A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…
Q: How is agar in the petri dish prepared to grow the bacterial specimen? explain in your own words.
A: Agar is a polymer made up of subunits of sugar galactose and is a component of cell walls of several…
Q: What is the correct shape of the unknown bacterium growing on your mannitol salt agar
A: Mannitol salt agar also known as MSA is commonly used selective agar in microbiology. This material…
Q: Why did we both stab and streak our blood agar plates when inoculating them with bacteria?
A: Blood agar is a nutrient medium which contain essential nutrients for bacterial growth and blood of…
Q: Could you spread plate in the usual Petri dish a 10mL inoculum?
A: The spread plate method is a technique to plate a liquid sample containing bacteria so that bacteria…
Q: Why should caps of glassware be loosened first before sterilization? [Answer in not more than 2…
A: Sterilization is defined as the process which removes , kills or deactivates all forms of life (in…
Q: why are psychrotrophic bacteria incubated at 7 degrees
A: Each species of microbe has a preferred temperature that lies somewhere between its characteristic…
Q: You collected a sample from a patient with a suspected STI. After Gram staining, you observed this…
A: The staining of bacteria is an important method of classifying the bacteria There could be multiple…
Q: What process would explain why the dyes spread through the agar?
A: Agar -- Agar is polymer and mixture of polysaccharides obtained from the cell wall of red algae .…
Q: How should agar plates be incubated? Why?
A: Incubating the plates to stimulate the growth of microbes is a crucial step in any microbiology…
Q: Why does dry heat require higher temperatures for longer time periods to sterilize than moist heat
A: Sterilization may be defined as the process which help in the complete removal of all…
Q: What things can be done during sample collection of a sick patient to help eliminate the culture of…
A: Normal flora is the organisms that are present in the body for normal functioning. The normal flora…
Q: how could you determine if there is any contamination on your agar?
A: Agar consists of two polysaccharides, agarose and agaropectin, but agarose making up about 70% of…
Q: What type of agar is MacConkey agar?
A: MacConkey agar or MAC which is also described as a bacterial culture medium was named after the…
Q: If my cultures are suffering severely due to alkaloid production and accumulation in the medium. In…
A: Alkaloids are basically the natural substances that can be used largely for human medication.…
Q: What is agar and what makes it the best agent for making culture media? explain
A: Microbiology is the branch of science which deals the study of different microorganisms such as…
Q: Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the…
A: Microorganisms are present in abundance in nature. When there are no aseptic conditions then…
Q: Why should you filter some liquids for sterilization, rather than autoclaving them?
A: To control microbial growth or contamination there are a number of aseptic techniques developed that…
Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…
A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…
Q: How do you do the continuous culture of bacteria by chemostat and turbidostat
A: Answer: CONTINUOUS CULTURE : It is the method of culturing/growing of bacteria in a media in a…
Q: During the preparation of a smear leading into simple staining of the bacterial culture S.…
A: S. epidermidis is a gram-positive bacteria.
Q: Which of the following types of media would be appropriate to use in order to culture a fastidious…
A: Answer:- From all the four above-mentioned medias yeast extract media and nutrient media are…
Q: You are reading culture plates today and identifying pathogens that grow from patient samples. Which…
A: The culture plates for growing microbes hold importance in understanding and examining the…
Q: What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
A: Bacterial culture media is a kind of growth media in which the required bacteria is cultured. The…
Q: If you suspect that your microbial culture growing in nutrient broth is contaminated, what can you…
A: The contamination refers to the growth of one or more undesired cultures in your pure sample. It can…
Q: Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies…
A: Microorganisms are small organism that cannot be seen by naked eyes. Microorganism can be harmful to…
Q: After the mordant has been added, Gram-positive organisms are stained __________ and Gram-negative…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?why is it important that a small amount of inoculum be used in preparation of an agar slant?Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?
- In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?why is it important that you disinfect your work area both before and after working with bacteria?What type of agar would you use to grow gram negative bacteria