3. The following absorbance spectrum was collected. What color is the sample solution? Explain using the color wheel. 1.4 1.2 1. 0.8- 0.6 0.4 0.2+ 300 400 500 600 700 800 wavelength (nm) Absorbance
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- Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/LA calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation
- I have: DMEM:F12 800X ITS 1.07 g/ml Linoleic acid Nu-serum (pure solution) BSA (pure powder) How much of each would I need to make 500ml of (1x ITS, 7.49 ug/ml linoleic acid, 4% v/v Nu-serum, 0.15% W/V BSA, and DMEM:F12 as the solvent)?1. compute for patient B result of his chloride analysis with the following data using mg/dL unit factor: 355/ mg/dL , Absorbance sample = 0.2148, Absorbance standard = 0.3017 a. 24.8 mg/L b. 25.27 mg/dL c. 2.7119 mmol/L d. 252.7 mg/dL 2. an adult patient suffering from debilitation is supected of calcium deficiency. His doctor requested for calcium anaylusis with the following data gathered standard known value is B: Absorabance is 0.2876. sample absorbance is 0.4291 compute for the calcium in mmol/L a. 53.6mmol/L b. 5.36 mmol/L c. 11.9 mmol/L d. 119mmol/L 3. calculate the value of sodium where the PREC solution has diclored. data gathered are the following: Absorbance of Reagent Blank = 0.3124 Absorbance of sample = 0.114, Absorbance of standard = 0.275 value is 150mmol/L a. 108.2 mmol/L b. 170.4 mmol/L c. 795.72 mmol/L d. 79.5 mmol/LA glucosamine solution of unknown concentration was diluted as follows: 1 mL of the solution was diluted to 5.0 mL. A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank). What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL? Given: Corrected absorbance = 0.395 y= 1.165x+0.055 Standard equation
- If a powder in a 2gm vial diluted with 6.8ml of diluent provides 8ml of solution and each ml contains 250mg of a drug, how many ml is needed to give 750mg.i) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).A 510 mL IV bag contains a 25% dextrose solution. How much of the original solution must be replaced with a 65% dextrose solution to increase the original concentration to 35%?
- i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precisionThe pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pHHow many mL of methadone is needed for a patient who was prescribed 0.015 g of methadone? The vial reads 6 milligrams of methadone per 1 mL of solution.