When a sample presents some matrix interference, which of the ff. are carried out? Dilute sample to minimize sample interference. Prepare a standard addition curve. If possible, remove interference prior to the quantification. Dilute sample until interference is completely removed.
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- Which of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.# of inversions needed: Match the additives in the rows to the number of inversions needed after collection of sampleWhy is it important that the standard curve you create in biological analyses with spectrophotometry is measured using specialty cuvettes?
- Which precise standards are you going to apply in order to identify your unknown? Make sure your RFP can be clearly distinguished from the other two using the criteria. lab intro attcehd if needed:From a water sample, you must determine the number of colonies present (CFU's/ml.). However, looking at the plates of the dilutions you find some cultures with scattered colonies. Briefly explain the reason for eliminating or not considering these plates in the data collection. Short answer please..Larger plate height corresponds to which of the following? a. Higher resolution b. Lower resolution c. Slower separation d. Faster separation
- What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).You look at a slide you prepared and, despite the fact that you know you put organism onto the slide in the first place, no organism remains on the slide at the end of the methylene blue staining. Identify TWO different problems in staining that could lead to this outcome.a) What is the dilution factor if you add a 1ml aliquot of a specimen to 99ml of diluent? b)How to make 500ml of a 1:250 dilution?
- Mary Jo prepared a series of diluted samples by taking 1 mL of sample and adding it to 4 mLs of diluent. She then diluted the sample further by adding 1 mL of diluted sample to 19 mLs of diluent. What is the final dilution factor ?Please use the image to answer the question, why are there only 2 bands for each sample?Why can we use drops instead of concentration to graph the data? What do we have to do experimentally to ensure reliable data? In other words, what equipment do we need to use to make sure the only thing changing is the concentration of bleach.