Which of the following best fit this spectroscopic data? All 'H NMR data shown as x.xx ppm (splitting), integration. 3.17 ppm (s), 3 7.42 ppm (d), 2 7.30 ppm (d), 2 1.49 ppm (d), 3 4.30 ppm (q). 1 PPM D
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- The following calibration data were obtained by an atomic absorption spectrophotometric (AAS) method for the determination of mercury in an herbal product. (show complete solution) 1. What is the detection limit for the method? 2. What is the limit of quantitation for the method?How can l exploit the mobile phase composition to optimize my HPLC separation?1) Calculate the molar extinction coefficient (molar absorptivity) of tartrazine in units of 1/(M·cm). Assume a cuvette path length of 1.00 cm. 2) If Ana determines the absorbance of an unknown tartrazine sample to be 0.662, what is the concentration of the sample in µM?
- Using The Tanabe-Sugano Diagram, Estimate Δ And B For (A) [Ni(OH2)6]2+ Absorptions At 8500, 15,400, And 26,000 Cm-1)(a) A sample of body serum is to be analysed for sodium by flame emission spectroscopy. 1.00cm3 aliquot of serum was pipetted into each of two 50.0 cm3 volumetric flasks. The firstflask was diluted to volume with deionised water. The absorbance of this first solution was0.350. To the second flask 10.0 cm3 of a 25.0 ppm sodium standard was added and theflask made up to volume with deionised water. The absorbance of this second solution was0.720.(i) Calculate the concentration of the sodium in the body serum in mg dm–3Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425 Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425a. Calculate the retention factor for each soluteb. Calculate the selectivity factorc. How many plates are needed to provide a resolution of 1.5?d. What column length should be used to provide a resolution of 1.5 if the plate height ofthe packing is 1.5 ×10-3 cm?e. If the flow rate is 6.75 cm min-1, how long will it take to elute the two species?
- The following calibration data were obtained by an atomic absorption spectrophotometric (AAS) method for the determination of mercury in an herbal product. 1. Calculate the calibration sensitivity.2. Calculate the coefficient of variation for the mean at 6.00 ppm 3. What is the detection limit for the method? 4. What is the limit of quantitation for the method?Calculate the amount of reagent needed to prepare 100 mL of a standard solution of 1000 ppm Au3+ in lN HCl for atomic absorption spectroscopy from standard HAuCl4.i) Find the differences between the AES and XPS in terms of principle and information obtained from the spectra. ii) Why AFM is mostly utilized microscopic method for the determination of the thickness of the film?
- I extracted caffeine by using chloroform and magnesium sulfate to dehydrate the organic phase. I got crude caffeine just by solvent evaporation at room temperature. However, I wanted to conduct Raman spectroscopy measurements but I did not get the characteristic peaks. I think that it has to be whit chloroform presence. So I let the crude caffeine 24h at 65 degrees but still I did not get any peak. What can I use to rinse my crude caffeine extract to remove all chloroform molecules? Or what is wrong with my extraction?I extracted caffeine by using chloroform and magnesium sulfate to dehydrate the organic phase. I got crude caffeine just by solvent evaporation at room temperature. However, I wanted to conduct Raman spectroscopy measurements but I did not get the characteristic peaks. I think that it has to be whit chloroform presence due to its fluorescence. So I let the crude caffeine 24h drying at 65 degrees but still I did not get any peak. What can I use to rinse my crude caffeine extract to remove all chloroform molecules? Or what is wrong with my extraction?Predict relative intensities at m/z 34, 35, and 36 for H2S. Let the intensity of the molecular ion be 100 and disregard contributions ,0.1%.
