Which of the following statements is true of plasmids? A Agrobacteria use plasmids to induce tumors in plant genomes Scientists use plasmids to introduce recombinant DNA into plants and animals O They are parasitic DNA that replicate in human cells. Bacteria are transformed when they take plasmids from their extra cellular environment A They are circular fragments of DNA that naturally occur in bacteria Bt corn expresses a toxin encoded on a plasmid

Which of the following statements is true of plasmids? A Agrobacteria use plasmids to induce tumors in plant genomes Scientists use plasmids to introduce recombinant DNA into plants and animals O They are parasitic DNA that replicate in human cells. Bacteria are transformed when they take plasmids from their extra cellular environment A They are circular fragments of DNA that naturally occur in bacteria Bt corn expresses a toxin encoded on a plasmid

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter17: Biotechnology And Genomics

Section: Chapter Questions

Problem 1VCQ: Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner...

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A.) Transformation is best described as: Group of answer choices The integration of foreign DNA into a genome The uptake of naked DNA from the environment The transfer of DNA via a bacteriophage Transfer of a plasmid from one organism to another B.) What is the function of the araC gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells C.) What is the function of the bla gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on…
Which of the following would be a good application of plasmid transformation? 1) to make copies of DNA 2) to isolate a change in a single nucleotide 3) to separate DNA fragments 4) to sequence DNA
Using one of the options below, finish the following statement so that it is correct. Plasmids are _________ a. extrachromosomal, circular molecules of RNA b. extrachromosomal, linear molecules of DNA c. None of these options are correct d. extrachromosomal, circular molecules of DNA e. extrachromosomal, linear molecules of RNA
Which of the following one is liable for the conversion of covalently closed circular DNA to super coiled DNA of the plasmid? a.Exonuclease b. DNA Gyras c. Endonuclease d. Topoisomerase
Which of the following would occur through specialized transduction?a. acquisition of Hfr plasmidb. transfer of genes for toxin productionc. transfer of genes for capsule formationd. transfer of a plasmid with genes for degrading pesticides
The following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloning
Which of the following statements is false?a) The cells which take up recombinant plasmids are drug resistantb) The cells with no recombinant plasmids are drug resistantc) Plasmid replication is independent of cell’s divisiond) The cells containing recombinant plasmid are called transformed cells
You isolated a 10,500 base pair plasmid (supercoiled, cccDNA) from E. coli. The plasmid contains one unique recognition site for EcoRI, a restriction enzyme. Restriction enzymes recognize a specific sequence and cut both strands of the DNA at that sequence (Chapter 7). Brief DNase I treatment breaks one (or very few) phosphodiesterbonds in each DNA molecule, leaving the double-stranded DNA with one strand broken but the other strand intact, i.e “nicked.” You briefly incubated the cccDNA at 37°C in four separate reactions containing the components listed below. You ran each reaction sample on an agarose gel and visualized the DNA using ethidium bromide and UV light. The reactions included the appropriate buffer and ATP when required. An agarose gel containing four lanes of possible products is given below. Please refer to Figs. 4-26 and 4-27 in Watson for reference. For each reaction, indicate which lane of the gel contains the products that you would expect to see on your…
We transformed E coli cells with a plasmid modified to contain a ‘virulence factor’ which would allow growth on media containing the antibiotic kanamycin (Kan). The plasmid confers constitutive resistance to ampicillin (Amp). The bacterial experiment is about understanding whether such a ‘virulence factor’ confers physiological adaptation to Kan or whether the development of resistance can be explained by random mutations. For each independent transformation we re-suspended the cells from three colonies in Luria broth. For each suspension of cells we plated 100 microliters on a Kan plate. To estimate the number of cells seeded on each Kan plate we made four serial dilutions that were plated on Amp plates (1 – 4) and we counted the number of cells growing on them. From this we extrapolated how many cells had been seeded on the Kan plate. Then we normalised the Kan results for all the plates, assuming that every plate had been seeded with 10[5] cells. Consider two Kan plates, each with…
A) A bacterium that has an F plasmid is able to do which of the following? Group of answer choices resist antibiotics survive in very harsh environments such as hypersaline lakes grow a cell wall form conjugation tubes and pass on their DNA to other bacteria reproduce sexually B) horizontal gene transfer refers to bacteria's ability to Group of answer choices make peptidoglycan for their cell walls transfer DNA to offspring, or "daughter" cells transfer DNA only when on horizontal surfaces, such as a table top transfer pieces of DNA to other cells in the population engage in mutualisms with certain species
Consider the image below. This image shows plasmid DNA isolated through exactly the same method that you used. The lanes that were untreated (TE) contain plasmid DNA, but also large amounts of smaller nucleic acids that are predicted to be RNA. What is one explanation for why RNA could be present in the sample that should have contained plasmid DNA only? Think about the chemical similarities of these two molecules.
A plasmid differs from the bacterial chromosome in that it is __________. A. significantly smaller B. always found as a single copy of DNA in a cell C. circular D. double-stranded A. significantly smaller B. always found as a single copy of DNA in a cell C. circular D. double-stranded