When plasmids are used to produce a desired proteinO the plasmids are inserted into the bacterial chromosome.O the plasmids multiply and produce the protein outside of the bacterium.O the desired gene is inserted into the plasmid, and the plasmid is returned to the bacterium by transformation.O the bacterial genome and plasmid are inserted into the genome of the cell containing the desired gene (often the cell of a plant or animal).

Plasmid are circular small double-stranded DNA molecules that exist independently of the host…

Answered: When plasmids are used to produce a…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.
A DNA sequence is shown below, which includes a gene as marked. You have the restriction enzymes SalI and HindIII available to you to excise the gene prior to its incorporation into a plasmid vector. Which would you use to excise the gene?
A biologist is attempting to clone the gene encoding a particular enzyme (Enz) into a plasmid vector in E.coli. This plasmid has a gene encoding a green fluorescent protein (GFP) as well as a gene for tetracycline antibiotic resistance (TetR). The restriction site (to clone foreign DNA into) is within the GFP sequence. Which of the following would be expected when trying to see which E. coli cells acquired the recombinant plasmid (i.e., carrying the Enz gene)? Bacteria UNABLE to grow on tetracycline-containing media AND are NOT able to make green fluorescent protein are the ones that contain the recombinant plasmid. Bacteria able to grow on tetracycline-containing media AND that are NOT able to make green fluorescent protein are the ones that contain the recombinant plasmid. Bacteria able to grow on tetracycline-containing media AND are able to make green fluorescent protein are the ones that contain the recombinant plasmid. Bacteria UNABLE to…
Which of the following one is liable for the conversion of covalently closed circular DNA to super coiled DNA of the plasmid? a.Exonuclease b. DNA Gyras c. Endonuclease d. Topoisomerase
A researcher has a Trp− auxotrophic strain of E. coliwith a mutation in a single gene. To identify thatmutant gene, she uses a genomic library made from a wild-type version of that same strain to find plasmids that rescue the mutant phenotype. The result issurprising. She recovers 10 plasmids that provide aTrp+ phenotype, but six of the plasmids contain geneX, while the other four contain gene Y. Our scientisthas encountered a phenomenon called multicopysuppression, related to the fact that plasmids are usually present in several copies per bacterium. Becausethe genes in the plasmids are present in more thantheir usual single copy in the bacterial chromosome,more than the usual amount of Protein X or Protein Yis being produced from the plasmids. Sometimes,overexpression of one protein can rescue the mutantphenotype caused by loss of a different protein.Suggest at least two ways that our scientist could determine which of the two genes, gene X or gene Y, actually corresponds to the…
A biotech lab technician is working with a mutated form of a particular bacterial gene and wants to obtain large amounts of plasmid DNA that contains this gene. In this situation, the gene should be in which of the following type of vector (to introduce into E. coli)? Expression vector. Shuttle vector. F plasmid. Cloning vector.
A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for kanamycin resistance (kanR). There is a restriction site inside of the strR gene that you will use to move in your gene of interest. After cutting both your vector and insert you mix them together in a test tube. You then transform your mixture into E. coli and plate your cells on a master plate without any antibiotics. After colonies appear, you pick and re-plate onto a streptomycin containing plate and onto a kanamycin containing plate. What do you expect to grow on the master plate without any antibiotics? What do you expect to grow on the streptomycin plate? What do you expect to grow on the kanamycin plate? How do you determine which colonies contain your gene of interest? As mentioned above, there is a restriction site found within the strR Please draw or write out two different examples of what restriction sites may look like. These should be short…
In the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give sticky ends. The DNA segments that are to be added to the plasmid are cleaved with the same restriction endonuclease. What aresticky ends and why is it important that the target DNA and the plasmid it will be incorporated into have complementary sticky ends?
Using one of the options below, finish the following statement so that it is correct. Plasmids are _________ a. extrachromosomal, circular molecules of RNA b. extrachromosomal, linear molecules of DNA c. None of these options are correct d. extrachromosomal, circular molecules of DNA e. extrachromosomal, linear molecules of RNA
If we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)
A linear plasmid contains only two genes, which are transcribed in opposite directions, each one from the end, toward the center of the plasmid. Draw diagrams ofa. the plasmid DNA, showing the 5′ and 3′ ends of the nucleotide strands.b. the template strand for each gene.c. the positions of the transcription-initiation sites.d. the transcripts, showing the 5′ and 3′ ends.
Which of the following statements is false? a) the bacterial chromosome is usually circular b) the bacterial chromosome has a single origin of replication c) the bacterial chromosome consists of a single molecule of DNA d) plasmids are small DNA molecules that occur in bacteria but are not essential - for normal function. e) Most bacterial genomes consist of fewer than 1,000 genes

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