Viscosity measurements were done on native form of Protein XYZ and on 4 denaturants. The crude Protein XYZ was diluted to produce 5% w/v. Using the Ostwald viscometer, the following data were obtained: time, sec time, sec time, sec time, sec Denaturant Blank (Native) Native Blank (Denaturant) Denatured pH= 2.00 70 95 70 101 Temp (90 °C) 70 98 70 150 0.1M Urea 72 97 88 110 DTT 71 96 90 170 a. Calculate and tabulate the reduced viscosities (in mL/g) of the native and denatured Protein XYZ. b. Determine two most effective denaturants. c. What can be inferred with the two most effective denaturants?

Viscosity measurements were done on native form of Protein XYZ and on 4 denaturants. The crude Protein XYZ was diluted to produce 5% w/v. Using the Ostwald viscometer, the following data were obtained: time, sec time, sec time, sec time, sec Denaturant Blank (Native) Native Blank (Denaturant) Denatured pH= 2.00 70 95 70 101 Temp (90 °C) 70 98 70 150 0.1M Urea 72 97 88 110 DTT 71 96 90 170 a. Calculate and tabulate the reduced viscosities (in mL/g) of the native and denatured Protein XYZ. b. Determine two most effective denaturants. c. What can be inferred with the two most effective denaturants?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

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Problem 42SST

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The main constituents in vinegar are water and ethanoic acid (CH3COOH). In order to determine the concentration of acid in homemade vinegar, a student titrated 25 cm3 of 001 M NaOH against the vinegar. The equation for the reaction is: CH3COOH(aq) + NaOH(aq) ® CH3COONa(aq) + H2O(l) The following titration results were obtained: Burette readings (cm3) Rough 1 2 Final burette reading 20.10 38.90 31.40 Initial burette reading 0.10 20.00 12.50 Volume of vinegar used 20.00 18.90 18.90 (a) What volume of vinegar should be used in the calculation? (b) What is the mole ratio of NaOH:CH3COOH? (c) Calculate the number of moles of alkali in 25 cm3 of NaOH solution used. (d) How many moles of acid were used in the titration? (e) Calculate the…
pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the % solubility of protein in the soy flour (Assume the soy flour contains 35% protein (w/w))
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	time, sec	time, sec	time, sec	time, sec
Denaturant	Blank (Native)	Native	Blank (Denaturant)	Denatured
pH= 2.00	70	95	70	101
Temp (90 °C)	70	98	70	150
0.1M Urea	72	97	88	110
DTT	71	96	90	170