Multiple choice. Which of the following is not a method that can be used to determine the 3° structure of proteins? (2 pts) Protein X-ray crystallography b) Circular dichroism c) Cryogenic electron microscopy d) Protein NMR

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter3: Biological Molecules: The Carbon Compounds Of Life
Section: Chapter Questions
Problem 8TYK: The first and major effect in denaturation of proteins is that: a. peptide bonds break. b. helices...
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8. Multiple choice. Which of the following is not a method that can be used to determine
the 3° structure of proteins? (2 pts)
a) Protein X-ray crystallography
b)) Circular dichroism
c) Cryogenic electron microscopy
d) Protein NMR
9. Multiple choice. Approximately what overall yield can you expect from the solid phase
peptide synthesis (SPPS) of a "10-mer" (a 10 amino acid peptide), if the yield from each
step is 85%? (2 pts)
23%
b) 20%
c) 85%
d) 71%
10. Multiple choice. Which protein purification method is best for an experiment in which
you want to purify a monomeric form of a protein away from a tetrameric form? (2 ptrs)
(-His
a) Affinity chromatography S
b) High pressure liquid chromatography
c) lon exchange chrommatography
d) Size exclusion chromatography
2.
I1. Short answer. What is the Edman degradation experiment useful for? (3 pts)
purdinate an for prry structers
steps .
Transcribed Image Text:8. Multiple choice. Which of the following is not a method that can be used to determine the 3° structure of proteins? (2 pts) a) Protein X-ray crystallography b)) Circular dichroism c) Cryogenic electron microscopy d) Protein NMR 9. Multiple choice. Approximately what overall yield can you expect from the solid phase peptide synthesis (SPPS) of a "10-mer" (a 10 amino acid peptide), if the yield from each step is 85%? (2 pts) 23% b) 20% c) 85% d) 71% 10. Multiple choice. Which protein purification method is best for an experiment in which you want to purify a monomeric form of a protein away from a tetrameric form? (2 ptrs) (-His a) Affinity chromatography S b) High pressure liquid chromatography c) lon exchange chrommatography d) Size exclusion chromatography 2. I1. Short answer. What is the Edman degradation experiment useful for? (3 pts) purdinate an for prry structers steps .
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