Which of these amino acid side chains would be effective at carrying out covalent catalysis in an enzyme-catalyzed reaction at pH 7.4? Assume that if more than 10% of the side chain can act as a strong nucleophile, then there will be a sufficient amount of nucleophile available for the enzyme to perform covalent catalysis. Please select all possible answers. Lysine side chain with pKa = 9.0, where the positively charged state is destabilized by a nearby arginine side chain Glutamate side chain with pKa = 6.5, where the negatively charged state is destabilized due to the presence of a nearby Asp de chain Histidine side chain with pKa = 5.5, where the positively charged state is stabilized due to the presence of a nearby Asp side chain Alanine side chain

Which of these amino acid side chains would be effective at carrying out covalent catalysis in an enzyme-catalyzed reaction at pH 7.4? Assume that if more than 10% of the side chain can act as a strong nucleophile, then there will be a sufficient amount of nucleophile available for the enzyme to perform covalent catalysis. Please select all possible answers. Lysine side chain with pKa = 9.0, where the positively charged state is destabilized by a nearby arginine side chain Glutamate side chain with pKa = 6.5, where the negatively charged state is destabilized due to the presence of a nearby Asp de chain Histidine side chain with pKa = 5.5, where the positively charged state is stabilized due to the presence of a nearby Asp side chain Alanine side chain

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter22: Proteins

Section: Chapter Questions

Problem 22.99P

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