Which photosystem (I, II, both or neither) is disrupted by each of the inhibitors? Explain how you arrived at your answer. DCMU Methylviologen Desaspidin Ferricyanide Phenazine methosulfate

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Which photosystem (I, II, both or neither) is disrupted by each of the inhibitors?
Explain how you arrived at your answer.
DCMU
Methylviologen
Desaspidin
Ferricyanide
Phenazine methosulfate
Objective: To reconstruct some of the experiments that were performed on
Photosystems I and II in order to determine whether they were coupled, and then
determine in what sequence the various electron carriers were used in transport.
1. Use the photosystem I and II diagram below, which is similar but not identical to
the one in the text. As in oxidative phosphorylation, in which high-energy electrons are
transferred during the production of stored chemical energy, photosynthesis can be
inhibited at different points within the system by a variety of chemicals.
Ferredoxin (F)
P700
cyt b6
●
plastocyanin (PC)
NADPH
NADP+ + H+
cyt f
ADP + Pi
ATP
cyt b559
DCMU prevents oxidation of water
Methylviologen accepts electrons from P700
H₂O
Plastiquinone (Q)
1/2 O₂ + 2 H+
P680
• Desaspidin uncouples phosphorylation
Ferricyanide accepts electrons from the end of the transport chain
ATP
ADP + P₁
Phenazine methosulfate accepts electrons from ferrodoxin and donates the electrons
directly to the transport chain
Transcribed Image Text:Objective: To reconstruct some of the experiments that were performed on Photosystems I and II in order to determine whether they were coupled, and then determine in what sequence the various electron carriers were used in transport. 1. Use the photosystem I and II diagram below, which is similar but not identical to the one in the text. As in oxidative phosphorylation, in which high-energy electrons are transferred during the production of stored chemical energy, photosynthesis can be inhibited at different points within the system by a variety of chemicals. Ferredoxin (F) P700 cyt b6 ● plastocyanin (PC) NADPH NADP+ + H+ cyt f ADP + Pi ATP cyt b559 DCMU prevents oxidation of water Methylviologen accepts electrons from P700 H₂O Plastiquinone (Q) 1/2 O₂ + 2 H+ P680 • Desaspidin uncouples phosphorylation Ferricyanide accepts electrons from the end of the transport chain ATP ADP + P₁ Phenazine methosulfate accepts electrons from ferrodoxin and donates the electrons directly to the transport chain
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