Q4. Why are some plates placed into a candle jar?Q6. What is the purpose of each kind of medium? INCUBATION of plates:All BHI or blood plates inside of a candle jar, as well as Mitis-salivarius agar plates. The candle jar is lit,closed and placed in 37 C incubator. Mannitol salt agar and MacConkey's plates are incubated in theregular 37 C incubator.Interpretation:1. Remove all plates from the incubators, and separate them into 2 groups---plaque or toothbrush.2. Determine the number of bacteria per plaque sample. This will not be per gram since you hadmuch less than a gram sample of plaque.3. Determine the number of bacteria per toothbrush head, using counts from blood or BHI.4. Determine the number of bacteria per toothbrush head for each group of bacteria, based on eachindividual media type.5. Analyze each type of medium for bacteria for BOTH toothbrush and plaque:• BAP or BHI - for total count of facultatives and aerobes• Mannitol salt - for salt-resistant Staphylococcus species, Staph aureus turns yellow (seeexercise in lab manual)MacConkey's - for enteric gram - rods, coliforms turn red and non-coliforms are clearishMitis-salivarius agar - small blue colonies (Strep mitis), large blue gumdrop-like colonies(Strep salivarius) and dark blue/black, shiny colonies (Enterococcus faecalis)6. Gram stain at least one colony on each type of medium and record the gram reaction,arrangement, and shape. Interpretation:> Determine the total number of bacteria per plaque sample and toothbrush head from BHI.Number of colonies on a selected plateCFU/ mL =Total dilution x amount platedResults of the plates using the plaque sample10110 2TMTCTMTCTMTC10238 colonies24 colonles2 colonies

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Biology

Why are some plates placed into a candle jar?

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter15: Antibiotic Agents

Section: Chapter Questions

Problem 18RQ

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Concept explainers

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.

Question

Q4. Why are some plates placed into a candle jar?

Q6. What is the purpose of each kind of medium?

INCUBATION of plates:
All BHI or blood plates inside of a candle jar, as well as Mitis-salivarius agar plates. The candle jar is lit,
closed and placed in 37 C incubator. Mannitol salt agar and MacConkey's plates are incubated in the
regular 37 C incubator.
Interpretation:
1. Remove all plates from the incubators, and separate them into 2 groups---plaque or toothbrush.
2. Determine the number of bacteria per plaque sample. This will not be per gram since you had
much less than a gram sample of plaque.
3. Determine the number of bacteria per toothbrush head, using counts from blood or BHI.
4. Determine the number of bacteria per toothbrush head for each group of bacteria, based on each
individual media type.
5. Analyze each type of medium for bacteria for BOTH toothbrush and plaque:
• BAP or BHI - for total count of facultatives and aerobes
• Mannitol salt - for salt-resistant Staphylococcus species, Staph aureus turns yellow (see
exercise in lab manual)
MacConkey's - for enteric gram - rods, coliforms turn red and non-coliforms are clearish
Mitis-salivarius agar - small blue colonies (Strep mitis), large blue gumdrop-like colonies
(Strep salivarius) and dark blue/black, shiny colonies (Enterococcus faecalis)
6. Gram stain at least one colony on each type of medium and record the gram reaction,
arrangement, and shape.

Interpretation:
> Determine the total number of bacteria per plaque sample and toothbrush head from BHI.
Number of colonies on a selected plate
CFU/ mL =
Total dilution x amount plated
Results of the plates using the plaque sample
101
10 2
TMTC
TMTC
TMTC
10
238 colonies
24 colonles
2 colonies

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