Q: Why is the onion mixture processed through the blender?
A: DNA extraction is the process of isolating DNA molecule from a biological sample. This was first…
Q: How was the process of bacterial transformation discovered?
A: The reception of bare DNA fragments from the external environment and the manifestation of that…
Q: How would restriction enzymes play a role in having an organismproduce a protein that it normally…
A: Restriction enzymes are specialized proteins produced by the bacteria that cut the DNA at a specific…
Q: The type of restriction enzymes that do not usually require ATP is : * О Туре ! О Туре I О Туре II O…
A: A restriction enzyme, also termed as a restriction endonuclease or restrictase, is an enzyme that…
Q: Define restriction point,
A: The cell cycle is the initial process that cells follow to prepare themselves for cell division.…
Q: What bacteria were used for the isolation of the three enzymes (HindIII, NdeI and PvuI)? What type…
A: HindIII is isolated from Haemophilus influenzae . NdeI is an isolated from Neisseria…
Q: ocedure to extract DNA through banana?
A: A nucleic acid called DNA is arranged into chromosomes. It has genetic data in it. Polynucleotide…
Q: Why is MgCl2 added to the PCR reaction?
A: De-novo gene synthesis is becoming an important tool for recombinant DNA technology. The Denovo gene…
Q: What stimulates the switch to the lytic pathway?
A: The bacteria that get infected by the viruses are known as bacteriophages. The virus inside the…
Q: What is the function of lysozyme in egg white?
A: Lysozyme is an enzyme ( glycoside hydrolase)that has bactericidal properties. It is present in human…
Q: Who discovered the plasmids?
A: Plasmid is an extrachromosomal small DNA molecule present in a cell. Plasmid exists separately from…
Q: How is Ti plasmid of Agrobacterium tumefaciens modified to convert it into a cloning vector?
A: Agrobacterium tumefaciens is a well-studied species of the gram-negative genus of bacteria, known as…
Q: What do restriction enzymes do
A: What do restriction enzymes do ?
Q: From what the chipko andolan was originated?
A: Chipko Andolab also known as chipko (means — hug) movement. This was nonviolent social ecological…
Q: Why doesn't the CRISPR system cut the bacterium's own DNA, at the point in its genome where it…
A: CRISPR is an acronym for Clustered Regularly Interspaced Short Palindromic Repeat. This name refers…
Q: Based on the restriction enzyme specificities given below, which will generate blunt ends?…
A: When a DNA fragment is subjected to the action of a restriction endonuclease enzymes, it cuts the…
Q: For sample 2, how many different size fragments will be generated after completing digestion with…
A: Introduction Enzymes are the crucial biomolecules which assists in various biochemical reactions…
Q: Is E.coli resistant to the antibiotic produced from the S. griseus strain?
A: Antibiotic resistance develops in bacteria by transferring an antibiotic-resistant gene from…
Q: steps in restriction digestion and PCR
A: Restrictions enzyme is use to cut DNA fragments. Two types of restrictions enzyme is found one is…
Q: Why is the catalase test useful for the differentiation of staphylococci from streptococci?
A: Bacteria are classified into two categories gram-positive bacteria and gram-negative bacteria.…
Q: From what other environments might E. coli bacteriophages be readily isolated?
A: Some of the prokaryotes, especially bacteria are infected with the viruses. These viruses are known…
Q: Why is it necessary to load the same amount of protein for each sample during electrophoresis?
A: Electrophoresis can be described as the movement of particles (charged particles) in a gel or fluid.…
Q: what is the function of restriction enzymes in digesting products in PCR
A: Restriction enzymes are also known as molecular scissors because they involves in digestion of…
Q: Based on the restriction enzyme specificities given below, which will generate blunt ends?…
A: The given restriction enzymes are endonucleases as they cut nucleotides at specific positions within…
Q: What uses of Type 1 Restriction Enzymes have if they seem to be so inaccurate?
A: Restriction enzymes are used to cut the DNA into fragments that are suitable for manipulation and…
Q: Define about restriction site ?
A: Recombinant DNA is made up of molecules of DNA from two different species that are inserted into a…
Q: What mechanisms account for restriction and modification?
A: Restriction-modification system is present in prokaryotic organisms like bacteria. Prokaryotic cells…
Q: How do the following factors affect the activity of restriction enzymes? pH conditions Mg2+…
A: Restriction enzymes (restriction endonucleases) are the enzymes that can cleave at specific sites in…
Q: What uses do Type 1 restriction enzymes have if they seem to be so inaccurate
A: Restriction enzymes refer to the restriction endonuclease enzymes that are used to cut DNA…
Q: What is the difference between the lytic pathway and the lysogenic pathway?
A: Lytic and Lysogenic cycles are the method of viral multiplication.
Q: Which are the Ten Commonly Used Restriction Enzymes?
A: A restriction enzyme could be a macromolecule that acknowledges a particular, short nucleotide…
Q: What are inducible enzymes ?
A: The inducible enzyme is also known as an adaptive enzyme. Opposite of inducible enzymes are…
Q: What are the optimal conditions for EcoRV and Aval restriction enzymes?
A: Restriction enzymes are also known as molecular scissors since they are used in cutting specific…
Q: Who isolated Restriction enzymes for the first time?
A: Restriction enzymes, also known as restriction endonucleases, recognize specific sites and cut DNA…
Q: What is a plasmid?
A: Prokaryotes are the less advanced organism as compared to the eukaryotes. They don't have well…
Q: What role did restriction endonucleases play in localizing the gene associated with cystic fibrosis?
A: The gene responsible for cystic fibrosis was isolated from restriction endonuclease digests.
Q: Does bacterial transformation occur in nature?
A: Transformation is the change in the genome of an organism. Transformation is one of the methods of…
Q: Restriction enzyme Restriction site GAATTC CTTAAG EcoRI BamHI GGATCC ССТAGG Hind III АAGCTT TTCGAA…
A: Restriction enzymes are enzymes that cleaves DNA into small fragments at or near specific…
Q: Define the concept of restriction-enzyme digestion ?
A: Enzymes are biocatalysts. These speed up biochemical reactions and are proteinaceous in nature.…
Q: What is micropropagation?
A: Tissue culture technique involves growing cells or tissues in an artificial medium, which is…
Q: What and where is the restriction site of the lacl gene of K. pneumoniae? What restriction enzyme…
A: The lacZ and lacI genes are transcribed from opposite strands in the lac operon of K. pneumoniae.…
Q: What is the difference between the total and faecal coliform
A: MPN assay is the statistical, multi-stage test for determining the presence of bacteria in water,…
Q: Are bacteriophages harmful ?
A: Bacteriophages are one type of virus. Every virus needs a host for reproduction. They are known as…
Q: What are the potential benefits and disadvantages of using bacteriophages in place of antibiotics?…
A: Compared to antibiotics, only a single phage is required to kill a single bacterium and so fewer…
Q: How do the following factors affect the activity of restriction enzymes? pH conditions Mg2+…
A: Restriction enzymes are those enzymes which cut the DNA at specific places so that they can be used…
Q: How many tested antibiotics targeted the folate pathway and DNA/RNA synthesis?
A: This action directs the examination of a distributed logical figure from a concentrate on whether…
Q: What is PID?
A: PID is Pelvic Inflammatory Disease.
Q: How would you ascertain if your restriction experiment was successful?
A: Restriction digest of plasmid DNA is done using the naturally occurring restriction enzymes that…
Why are the restriction enzymes added last to the digestion mixtures?
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