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1. Describe the three different type of hemolysis that are observed on blood agar.
2. What is a selective medium?
3. What is a differential medium?
4. Which media can be used to isolate E. coli samples from contaminated lettuce?
5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?
6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.
7. What is the color or TSI for Salmonella?
8. What is a fastidious organism?
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- 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.1. Differentiate the tubed media. 2. Discuss the different streaking methods. 3. What is the streaking method used for Antimicrobial Susceptibility testing? 4. How will you determine if a culture is pure or mixed?NOTE: Kindly provide references. 1. Differentiate between defined and undefined culture media. 2. Define alpha, beta, and gamma-hemolytic reactions on blood agar media (BAM). Cite two (2) examples of bacteria causing the above hemolytic reactions.
- An outbreak of Methicillin-resistant Staphylococcus aureus (MRSA) in the surgical ward at the hospital forces the hospital administrator to cancel and postpone all non-essential surgeries until the source of infection has been located and for measure put into place. Bacterial samples obtained from swab of wad staff equipment and surfaces arr brought to the lab for analysis. 1. What testing method will you employ to determine the level of exposure? 2 How would a positive test present? 3. How would a negative test present? 4. What recommendations would you make in your report?1. Illustrate in a diagram the identification scheme from gram-positive cocci.2. Discuss the principle involved in the biochemical tests in identifying species of staphylococci.3. Illustrate in a diagram the identification scheme for Streptococci.4. Discuss the principle involved in the biochemical tests in identifying species of Streptococci.A patient with a diagnosis of "Staphylococcal pneumonia" was admitted to the clinic. For successful etiological treatment we should select an effective antibiotic. For this purpose, was recommended to determine the antibioticogram of the pathogen. 1. What method can be used to determine antibiotic sensitivity? 2. The principle of the method and accounting for results.
- Hi, I want to ask the question related to antibiotics resistance? The question is the non-existance of solidifying agent may produce a liquid like culture media. Name the method used to determine the Antibiotic Susceptibility test which uses the said media? Can explain answer? Thank you.(1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specific1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic technique important in urine collection for culture? 3. What are the specimen consideration you have to remember in preparing blood culture? 4. Being a medical technology student, how can you contribute to avoid water pollution and degradation of natural resources?
- 1-purpose of Acid-Fast Method. 2-brief explanation of positive and negative results. 3-Predicted Results of staphylococcus epidermis, Mycobacterium smegmatis.Answer the following: Why do we need to transport the throat swab using transport culture medium?1. The selective ingredient in mannitol salt agar is ______________, which selects for the growth of Staphylococcus organisms.