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2. Why can't we say "sterile" technique?
3. How are aseptic technique similar and different in the lab and healthcare field? Be specific and explain at least two differences and two similarities.
4. You are asked to develop a method to transfer an unknown organism from a liquid broth to a solid petri dish. List each step that you would have to take. Be specific.
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- (1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specific(2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specific3. How are aseptic technique similar and different in the lab and healthcare field? Be specific and explain at least two differences and two similarities. 4. You are asked to develop a method to transfer an unknown organism from a liquid broth to a solid petri dish. List each step that you would have to take. Be specific
- 1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.I need help with microbiology/2010 1. Describe where the following items should be discarded: a) gloves b) petri dishes c) test tubes d) microscope slides 2. Describe the safety procedures for the following hypothetical situations: a. You spilled a full test tube of bacterial culture on the bench top. b. You notice flames coming from the tubing of your Bunsen burner
- 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.Explain shortly the concept of differential centrifugation and how you would limit pellet contamination which is a disadvantage of this technique.1. how does colony appearance and location with respect to the agar differ on the spread and pour plate? 2. list two additional factors that contribute to error in the spread plate method:
- Give 2 different reasons that scientists might need to isolate a pure culture.2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)The Centers for Medicare and Medicaid Services (CMS) have initiated revised policies regarding reimbursement to hospitals for care of patients who suffer health care-associated infections (HAIs) such as SSIs. Hospitals must bear the costs of treatment. Health care workers are the key in preventing patient injury and protecting hospitals. What is an example of a routine procedure performed by surgical technologists prior to entering the sterile field that would be part of the aseptic technique?
