he result of a TLC experiment showed that Compound X has an Rf values of 0.25 on a silica plate using 10% ethyl acetate in hexane as the mobile phase. Indicate if the Rf values of Compound X would be higher or lower in the following solvents:

he result of a TLC experiment showed that Compound X has an Rf values of 0.25 on a silica plate using 10% ethyl acetate in hexane as the mobile phase. Indicate if the Rf values of Compound X would be higher or lower in the following solvents:

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter7: Components Of Optical Instruments

Section: Chapter Questions

Problem 7.11QAP: Why is glass better than fused silica as a prism construction material for a monochromator to be...

See similar textbooks

Related questions

Q: a) Analyze the importance of dropping mercury electrode on polarography. (b) (i) Calculate the…

A: a) Importance of dropping mercury electrode on polarography : Dropping mercury electrode is a…

Q: What complications would a dried-out column (solvent level is below the top of the silica) introduce…

A: Answer

Q: What are the similarities and differences between a chromatogram from an HPLC with a diode array…

A: A chromatogram formed after chromatography technique. A chromatography is a technique to separate…

Q: We use reverse phase column chromatography with a non-polar C18 stationary phase and a mixture of…

Q: What adsorbents are typically used in TLC? On a TLC, a compound traveled 3.5 cm when the solvent…

A: Thin layer chromatography is the technique that is used to separate or purify the compound from a…

Q: Which statements are true for molecular exclusion chromatography?I Molecular exclusion…

A: ✓As the name ,Size-exclusion chromatography, also known as molecular sieve chromatography, is a…

Q: The image below shows van Deemter curves for five different HPLC columns used under the same…

Q: Which statement is true? Statement 1: Since tiny amounts of material are exposed on an open…

A: The answer of the above question is given in step 2

Q: 30) The separation efficiency of capillary columns over packed columns 31) The most common mobile…

A: Ans. (30) The separation efficiency of capillary columns over packed columns in GC is attributable…

Q: mixture of three components is separated using paper chromatography, and each component is analyzed…

A: Given: Absorbance of standard solution- Compound A = 0.72 Compound B = 0.55 Compound C = 0.80 For…

Q: What characteristic makes a photodiode array spectrophotometer suitable for measuring the spectrum…

A: Ans: 1- Characteristics The following characteristic makes a photodiode array spectrophotometer…

Q: In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If…

A: To ensure that the colours separate into independent bands that do not overlap as they migrate up…

Q: Column Chromatography) What complications would a dried-out column (solvent level is below the top…

A: column chromatography is the technique used to separate compounds of different polarity .In this…

Q: You first separated the components of spinach and then identified them by TLC analysis. Was there a…

A: TLC of spinach means separation of chlorophyll pigment.The different form of chlorophyll found in…

Q: A) What pH should the solvent system be to perform TLC on a sample suspected of containing…

A: A) Because the putative molecule is slightly acidic, we must utilize a solvent solution that is very…

Q: An analysis of a sample by TLC was used on a silica plate and a non-polar mobile phase revealed 2…

A: Thin layer chromatography TLC is a technique which is used for the separation and identification of…

Q: ___________ is a method which can be used to separate enantiomers. Column chromatography Proton…

A: Given: Enantiomers To find: Method use to separate enantiomers Solution: Enantiomers are those…

Q: Briefly discuss the factors that lead to band broadening in chromatography and highlight how these

Q: Describe with illustrations the observed peak for a chromatogram with the following tailing factors…

A: Tailing factor: It should meet the requirements of the individual monograph and can be calculated by…

Q: The following Rf values were computed from a normal phase chromatography experiment. Arrange them…

A: In the normal phase chromatography it contains polar as mobile phase and non polar as stationary…

Q: An LC separation specifies that the mobile phase be 25% tetrahydrofuran (THF) in water.…

Q: 1. What is the significance of Rf values? 2. What is the basis for the separation of components of…

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Which of the following characteristics of a reversed – phase chromatography? I. The stationary…

A: In reversed-phase chromatography is a liquid chromatography technique that involves the separation…

Q: The result of a TLC experiment showed that Compound X has an Rf values of 0.25 on a silica plate…

A: The Rf value of a compound on a TLC plate is calculated by the formula, Rf=Distance travelled by the…

Q: For indirect iodometric analysis of copper oxide, why did you not need to accurately weight out ki…

Q: The following Rf values were computed from a normal phase chromatography experiment. Arrange them…

Q: It is usually recommended to clean the glass surfaces of a refractometer with ethanol or isopropyl…

A: Refractometer is a device that measure the degree to which light changes direction called as angle…

Q: What are the disadvantages of uses the Thick layer stationary phase in Thin layer Chromatography…

A: Thin layer chromatograpy is one of the conventional technique for the separation of mixture of…

Q: What happens if the solvent concentration falls below that of the silica stationary phase? What is…

A: The chromatography technique consists of two phases mobile and stationary phase .Mobile phase is the…

Q: Would a component with a larger Rf value have a higher or lower affinity for the mobile phase…

A: TLC is an analytical technique which can be used to monitor the organic reaction transformations.…

Q: Gadolinium is used as a contrast agent in MRI. Which magnetic state of matter is characteristic of…

A: A compound or ion is said to be paramagnetic if it contains unpaired electrons in its outermost…

Q: The result of a TLC experiment showed that Compound X has an Rf values of 0.25 on a silica plate…

A: TLC is based on the principle of separation through adsorption type. The separation relies on the…

Q: Which of the following is/are characteristic of silica as stationary phase in chromatography? O It…

Q: For each of the following statements, answer TRUE or FALSE and explain your answer briefly. It…

A: True Potassium Dichromate solutions (235 - 430 nm)The most widely recognised reference material for…

Q: Indicate the order of elution in which the following compounds would be eluted from an HPLC column…

A: Concept: In reversed phase packing HPLC more polar compound will elute first. So we have to know the…

Q: the efficiency of a chromatographic column improves the measure that a.increases the height of the…

A: To determine what improves the efficiency of a chromatographic column:

Q: Which of the following cannot be used as adsorbent in column chromatography? a. Silica gel…

Q: TGA analysis

A: Ans. Thermogravimetric analysis or TGA techniques helps in determination of thermal stability of…

Q: -24. The following data are for a liquid chromatographic column: Length of Packing Flow rate VM Vs A…

A: Given, Chromatic mixture of species A,B,C and D. Length of packing , L =24.7 cm

Q: In general, we want to have absorptions less than or equal to 1. Given that an ethanol solution of…

A: • According to Beer-Lambert law when a monochromatic light having intensity I is passed through the…

Q: TLC was used to compare the solubility of two compounds A and B in a solvent system (acetic acid,…

Q: 1: The following TLC plate was prepared with the stationary phase as silica gel (polar, SiO₂) and…

A: In thin-layer chromatography, the retention factor (Rf) is used to compare and help identify…

Q: Which of the following statements about the SF experiment is/are CORRECT? The monochromator is…

A: A multiple choice question based on analytical process that is to be accomplished.

Q: Ion exchange chromatography can be used to separate a mixture of cations and anions, true or false?

A: Chromatography is the method used in laboratories for the separation of elements or molecules.

Q: You perform a thin-layer chromatography test on pigments extracted from a purple flower. There are…

A: Thin-layer chromatography, most commonly known as TLC, is one of the most important separation…

Q: In a GC experiment, a 30.00 m long fused silica capillary column produced 150,000 theoretical…

A: Calculate the plate height for the given problem which contains number of plates, length and height…

Q: a) Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) can be used as…

A: The solution of the question is given below:

Q: . Reversed phase HPLC refers to the condition of stationary phase and a mobile phase of separation.…

Q: Match the following chromatographic techniques with these corresponding principles of separation…

A: ✓chromatography, technique for separating the components, or solutes, of a mixture on the basis of…

Question

The result of a TLC experiment showed that Compound X has an Rf values of 0.25 on a silica plate using 10% ethyl acetate in hexane as the mobile phase. Indicate if the Rf values of Compound X would be higher or lower in the following solvents: HEXANE

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Introduction

VIEW

Explanation

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

The result of a TLC experiment showed that Compound X has an Rf values of 0.25 on a silica plate using 10% ethyl acetate in hexane as the mobile phase. Indicate if the Rf values of Compound X would be higher or lower in the following solvents: TOLUENE
More polar molecules move more slowly through silica than less polar molecules. Why is silica gel chromatography a good method of separating methyl orange from biphenyl?
An analysis of a sample by TLC was used on a silica plate and a non-polar mobile phase revealed 2 spots of ultraviolet light. The first spot shifted 3.2 cm, the second 5.6 cm, and the mobile phase 6.8 cm. calculate the Rf of the compound with the highest polarity
Which of the following is/are characteristic of silica as stationary phase in chromatography? O It has higher sample capacity. O It has wider range of most useful forms. O It is polar. O All of the above
a) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids shown below using silica gel as the stationary phase and ethyl acetate/hexanes as the mobile phase? Whichsolid would have the highest Rf and which would have the lowest Rf? Explain b) Would changing the mobile phase (from part a) and increasing thepolarity by introducing small amount of methanol to the mobile phase change the order ofRf of the above 3 compounds? Explain c) Another type of chromatography is Reverse Phase Chromatography. In this type ofchromatography, the stationary phase is non-polar and the mobile phase is polar. If astudent performed Reverse Phase TLC on the mixture of 3 solids (from part a),what would be the order of Rf’s of the compounds? Which would have the lowest Rf andwhich would have the highest Rf?
Ion exchange chromatography can be used to separate a mixture of cations and anions, true or false?
TLC was used to compare the solubility of two compounds A and B in a solvent system (acetic acid, methanol, H2O). The distances that solvent has moved in the silica gel TLC was 12 cm. and for the compounds were 2 and 8 cm respectively. (a) Calculate the Rf values and which compound is more soluble and which one is more polar. (b) What are the factors affecting the Rf value
In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?
Question: What is the effect of mobile phase composition on the separation efficiency of a reversed-phase chromatography system for the analysis of complex pharmaceutical mixtures
For indirect iodometric analysis of copper oxide, why did you not need to accurately weight out ki used in the standard and unknown analysis?
Anion and cation chromatography can be achieved by modifying? A. Type of dye B. Mobile phase C. Wavelength D. Stationary phase
Which statements are true for molecular exclusion chromatography?I Molecular exclusion chromatography separates on the basis of size.II Small molecules that freely pass through the stationary phase, Kav = 0.III Small molecules can fit in the pores of the stationary phase and effectively pass through a larger volume, and elute last.IV The pores in the stationary phase are too small for large molecules to pass through, so large molecules elute last.V Molecular exclusion chromatography with a hydrophilic stationary phase and an aqueous solvent is called gel permeation chromatography. A. II and V B. I, II, and IV C. III, IV, and V D. No correct combinations given above E. I, III, and IV F. I, II, III, and V